################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 691 GTAC GT^AC AseI 148 ATTAAT AT^TAAT AsnI 148 ATTAAT AT^TAAT Asp700I 334 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 965 GAGCAC G[AT]GC[AT]^C AspI 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 125 CCCGGG C^[CT]CG[GA]G BclI 206 TGATCA T^GATCA BfaI 574 CTAG C^TAG BsaBI 648 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 171 GGTCTC GGTCTC(1/5) BsiHKAI 965 GAGCAC G[AT]GC[AT]^C BsmAI 172 GTCTC GTCTC(1/5) BsoBI 125 CCCGGG C^[CT]CG[GA]G BspHI 246 TCATGA T^CATGA BspMI 808 GCAGGT ACCTGC(4/8) BsrBI 68 CCGCTC CCGCTC(-3/-3) BssSI 11 CACGAG CACGAG(-5/-1) DraII 1132 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1223 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1132 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 927 GATATC GAT^ATC FauI 1056 GCGGG CCCGC(4/6) FbaI 206 TGATCA T^GATCA HaeIII 1059 GGCC GG^CC HapII 126 CCGG C^CGG HpaII 126 CCGG C^CGG MaeI 574 CTAG C^TAG MamI 648 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1054 CAGCGG C[AC]G^C[GT]G MspI 126 CCGG C^CGG PflFI 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1119 GACTC GAGTC(4/5) PpuMI 1132 AGGACCC [GA]G^G[AT]CC[CT] PshBI 148 ATTAAT AT^TAAT RcaI 246 TCATGA T^CATGA RsaI 691 GTAC GT^AC SfaNI 653 GCATC GCATC(5/9) SmaI 125 CCCGGG CCC^GGG SspI 501 AATATT AAT^ATT TaqI 319 TCGA T^CGA TfiI 648 GATTC G^A[AT]TC Tth111I 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 319 TCGA T^CGA XmaCI 125 CCCGGG C^CCGGG XmaI 125 CCCGGG C^CCGGG XmnI 334 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 530 GAATTC [GA]^AATT[CT] 1003 GAATTC AcyI 751 GACGCC G[GA]^CG[CT]C 1218 GACGCC AlwNI 405 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 1111 CAGGAGCTG ApoI 530 GAATTC [GA]^AATT[CT] 1003 GAATTC AvaII 263 GGACC G^G[AT]CC 1133 GGACC BanI 39 GGCACC G^G[CT][GA]CC 577 GGCACC BbsI 165 GAAGAC GAAGAC(2/6) 1188 GAAGAC BcnI 125 CCCGG CC^[GC]GG 126 CCGGG BmyI 743 GTGCCC G[AGT]GC[ACT]^C 965 GAGCAC BpmI 557 CTGGAG CTGGAG(16/14) 738 CTGGAG BpuAI 165 GAAGAC GAAGAC(2/6) 1188 GAAGAC BsaHI 751 GACGCC G[GA]^CG[CT]C 1218 GACGCC Bsp1286I 743 GTGCCC G[AGT]GC[ACT]^C 965 GAGCAC BstAPI 358 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 967 GCACACGCTGC BstF5I 330 GGATG GGATG(2/0) 1167 GGATG EarI 946 GAAGAG CTCTTC(1/4) 1237 GAAGAG EcoRI 530 GAATTC G^AATTC 1003 GAATTC FokI 330 GGATG GGATG(9/13) 1167 GGATG HgaI 751 GACGC GACGC(5/10) 1218 GACGC Hin1I 751 GACGCC G[GA]^CG[CT]C 1218 GACGCC HinfI 648 GATTC G^A[ACGT]TC 1119 GACTC Ksp632I 946 GAAGAG CTCTTC(1/4) 1237 GAAGAG MseI 149 TTAA T^TAA 623 TTAA MslI 267 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 304 CATTCCTGTG NciI 125 CCCGG CC^[GC]GG 126 CCGGG NlaIII 247 CATG CATG^ 903 CATG Tru9I 149 TTAA T^TAA 623 TTAA XcmI 176 CCACGATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 755 CCATTTATTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AlwI BamHI BbvI BsaJI BsiYI BslI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI XhoII