(Circular) (Six-base) MAPSORT of: pFN21AE2726.seq Check: 3273 from: 1 to: 5971 pFN21AE2726 5971 bp With 182 enzymes: SgfI * July 3, 2018 16:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2804 2804 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3212 4034 5418 5686 1632 Size: 1580 822 1384 268 1917 Fragments arranged by size: 1917 1580 1384 822 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 2276 1166 Size: 1110 4861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2415 3121 3714 5286 2415 Size: 706 593 1572 3100 Fragments arranged by size: 3100 1572 706 593 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2535 3008 3280 3506 3658 5767 1493 Size: 1042 473 272 226 152 2109 1697 Fragments arranged by size: 2109 1697 1042 473 272 226 152 AseI AT'TA_AT Cuts at: 160 1032 2154 4525 160 Size: 872 1122 2371 1606 Fragments arranged by size: 2371 1606 1122 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2130 3296 1870 Size: 73 187 1166 4545 Fragments arranged by size: 4545 1166 187 73 BamHI G'GATC_C Cuts at: 2059 2819 2868 3301 3668 2059 Size: 760 49 433 367 4362 Fragments arranged by size: 4362 760 433 367 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2582 3292 4747 618 Size: 325 217 627 257 538 710 1455 1842 Fragments arranged by size: 1842 1455 710 627 538 325 257 217 BanII G_rGCy'C Cuts at: 1391 3290 1391 Size: 1899 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2177 3200 928 Size: 971 278 1023 3699 Fragments arranged by size: 3699 1023 971 278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 2211 1548 Size: 663 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2752 4032 5188 1790 Size: 962 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 962 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2425 2540 3702 Size: 416 53 83 399 24 1008 115 1162 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 1162 1008 958 416 399 115 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2939 2939 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2583 2764 4631 1239 Size: 1344 181 1867 2579 Fragments arranged by size: 2579 1867 1344 181 Bpu10I CC'TnA_GC Cuts at: 2903 2903 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2182 4634 882 Size: 617 683 2452 2219 Fragments arranged by size: 2452 2219 683 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 2657 5965 2657 Size: 3308 2663 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 3224 3778 4160 Size: 53 83 186 936 1224 467 554 382 Cuts at: 4160 4795 275 Size: 635 1451 Fragments arranged by size: 1451 1224 936 635 554 467 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 1178 Size: 30 165 30 808 30 4908 Fragments arranged by size: 4908 808 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5971 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2974 4032 4117 5188 2974 Size: 1058 85 1071 3757 Fragments arranged by size: 3757 1071 1058 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 2974 3290 4032 4117 5188 1391 Size: 399 962 222 316 742 85 1071 2174 Fragments arranged by size: 2174 1071 962 742 399 316 222 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 2251 3860 5590 2251 Size: 1609 1730 2632 Fragments arranged by size: 2632 1730 1609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 3326 844 Size: 1960 522 3489 Fragments arranged by size: 3489 1960 522 BsrBI CCG'CTC Cuts at: 1394 1823 2075 3858 1394 Size: 429 252 1783 3507 Fragments arranged by size: 3507 1783 429 252 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 2016 4031 5043 2016 Size: 2015 1012 2944 Fragments arranged by size: 2944 2015 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2369 2978 794 Size: 1575 609 3787 Fragments arranged by size: 3787 1575 609 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2059 2819 2868 3301 3668 4053 4070 5522 Size: 87 760 49 433 367 385 17 1452 Cuts at: 5522 5608 5620 5966 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 760 433 385 367 346 87 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2104 2139 529 Size: 35 1540 35 4361 Fragments arranged by size: 4361 1540 35 35 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DraIII CAC_nnn'GTG Cuts at: 3233 3233 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 3237 3901 1353 Size: 1593 291 664 3423 Fragments arranged by size: 3423 1593 664 291 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2583 2764 3212 4034 4631 5418 5686 Size: 393 951 181 448 822 597 787 268 Cuts at: 5686 1239 Size: 1524 Fragments arranged by size: 1524 951 822 787 597 448 393 268 181 EcoO109I rG'GnC_Cy Cuts at: 3138 3138 Size: 5971 EcoRI G'AATT_C Cuts at: 2535 3008 3280 3658 2535 Size: 473 272 378 4848 Fragments arranged by size: 4848 473 378 272 EcoRV GAT'ATC Cuts at: 2934 2934 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 3193 3225 4612 Size: 32 135 32 479 32 1110 32 1387 Cuts at: 4612 4644 4686 4718 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1387 1110 479 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2548 5085 5269 1305 Size: 1243 2537 184 2007 Fragments arranged by size: 2537 2007 1243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2313 3929 4288 4447 518 Size: 648 1110 37 1616 359 159 2042 Fragments arranged by size: 2042 1616 1110 648 359 159 37 MspA1I CmG'CkG Cuts at: 1606 3061 3387 4064 5212 5457 1606 Size: 1455 326 677 1148 245 2120 Fragments arranged by size: 2120 1455 1148 677 326 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3138 3138 Size: 5971 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3319 4454 5135 5326 826 Size: 219 2274 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 2132 3298 1872 Size: 260 1166 4545 Fragments arranged by size: 4545 1166 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 2508 3895 5 Size: 47 2456 1387 2081 Fragments arranged by size: 2456 2081 1387 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 3410 513 Size: 552 2345 3074 Fragments arranged by size: 3074 2345 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2695 4217 5929 Size: 274 80 33 51 1443 718 1522 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1712 1522 1443 718 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 5931 5963 2211 Size: 32 3688 32 2219 Fragments arranged by size: 3688 2219 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3142 1316 Size: 1826 4145 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2767 2188 Size: 579 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 2130 3296 1870 Size: 260 1166 4545 Fragments arranged by size: 4545 1166 260 XmnI GAAnn'nnTTC Cuts at: 2343 4100 2343 Size: 1757 4214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI