Restriction map for
pFN21AE9253
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# NO CUT ENZYMES ( 58 enzymes) #
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AatII AccIII AclI Alw44I ApaI
ApaLI AscI AvrII BamHI BbeI
BcgI BlnI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI BstBI ClaI
CpoI DrdI EagI EclXI Eco52I
FseI HpaI KasI KspI MluI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PmeI Psp1406I
PvuI RsrII SacII SalI SfoI
SfuI SgrAI SmaI SnaBI SspBI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 5271 GGTACC G^GTACC
AcyI 1056 GGCGTC G[GA]^CG[CT]C
AflIII 2051 ACGTGT A^C[GA][CT]GT
AgeI 2249 ACCGGT A^CCGGT
Aor51HI 9060 AGCGCT AGC^GCT
Asp700I 6040 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5271 GGTACC G^GTACC
BbrPI 5633 CACGTG CAC^GTG
BbvCI 1428 GCTGAGG CCTCAGC(-5/-2)
BciVI 8496 GGATAC GTATCC(6/5)
BcnI 623 CCCGG CC^[GC]GG
BsaAI 5633 CACGTG [CT]AC^GT[GA]
BsaHI 1056 GGCGTC G[GA]^CG[CT]C
BsrBI 6436 GAGCGG CCGCTC(-3/-3)
Bst1107I 1068 GTATAC GTA^TAC
BstEII 6829 GGTGACC G^GT[ACGT]ACC
BstPI 6829 GGTGACC G^GT[ACGT]ACC
BstZ17I 1068 GTATAC GTA^TAC
Eco47III 9060 AGCGCT AGC^GCT
EcoO65I 6829 GGTGACC G^GT[ACGT]ACC
EcoRV 5302 GATATC GAT^ATC
HaeII 9060 AGCGCT [GA]GCGC^[CT]
HgaI 1057 GCGTC GACGC(5/10)
Hin1I 1056 GGCGTC G[GA]^CG[CT]C
KpnI 5271 GGTACC GGTAC^C
MfeI 7987 CAATTG C^AATTG
MunI 7987 CAATTG C^AATTG
NciI 623 CCCGG CC^[GC]GG
NdeI 3104 CATATG CA^TATG
PinAI 2249 ACCGGT A^CCGGT
PmaCI 5633 CACGTG CAC^GTG
PmlI 5633 CACGTG CAC^GTG
PpuMI 5099 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 8588 AGTACT AGT^ACT
SexAI 8051 ACCAGGT A^CC[AT]GGT
SfiI 6273 GGCCTTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XmnI 6040 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 1068 GTATAC GT^[AC][GT]AC
7574 GTAGAC
AccII 613 CGCG CG^CG
6433 CGCG
AviII 393 TGCGCA TGC^GCA
3621 TGCGCA
BanI 5271 GGTACC G^G[CT][GA]CC
9175 GGCACC
BsaBI 5065 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6741 GATACAGATC
BsaWI 2249 ACCGGT [AT]^CCGG[AT]
3094 ACCGGA
BspMI 2543 ACCTGC ACCTGC(4/8)
8838 ACCTGC
BsrDI 618 CATTGC GCAATG(2/0)
5998 CATTGC
BsrFI 2249 ACCGGT [GA]^CCGG[CT]
4676 ACCGGC
BstUI 613 CGCG CG^CG
6433 CGCG
Cfr10I 2249 ACCGGT [GA]^CCGG[CT]
4676 ACCGGC
DraIII 2047 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
4129 CACCCTGTG
FspI 393 TGCGCA TGC^GCA
3621 TGCGCA
MamI 5065 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6741 GATACAGATC
MvnI 613 CGCG CG^CG
6433 CGCG
NheI 5734 GCTAGC G^CTAGC
7935 GCTAGC
PflMI 1221 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
9112 CCAACAGCTGG
SbfI 1302 CCTGCAGG CCTGCA^GG
6222 CCTGCAGG
SpeI 1026 ACTAGT A^CTAGT
3181 ACTAGT
Sse8387I 1302 CCTGCAGG CCTGCA^GG
6222 CCTGCAGG
Van91I 1221 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
9112 CCAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AciI AcsI AfaI AflII AhdI
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspEI AspHI AspI
AvaI AvaII BalI BanII BbsI
BbvI BclI BfaI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PaeR7I PflFI
PleI PshAI PshBI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XhoII