################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MamI MluI MslI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 493 GAATTC [GA]^AATT[CT] AgeI 755 ACCGGT A^CCGGT Aor51HI 29 AGCGCT AGC^GCT ApoI 493 GAATTC [GA]^AATT[CT] Asp700I 365 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 42 CCCGGG C^[CT]CG[GA]G AvaII 656 GGACC G^G[AT]CC BalI 338 TGGCCA TGG^CCA BglI 14 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 956 AGATCT A^GATCT BsaAI 121 TACGTG [CT]AC^GT[GA] BseRI 700 GAGGAG GAGGAG(10/8) BsoBI 42 CCCGGG C^[CT]CG[GA]G BspLU11I 1013 ACATGT A^CATGT BsrBI 139 GAGCGG CCGCTC(-3/-3) BsrFI 755 ACCGGT [GA]^CCGG[CT] BstAPI 146 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 956 AGATCT [GA]^GATC[CT] CfoI 30 GCGC GCG^C Cfr10I 755 ACCGGT [GA]^CCGG[CT] EaeI 338 TGGCCA [CT]^GGCC[GA] EarI 907 GAAGAG CTCTTC(1/4) Eco47III 29 AGCGCT AGC^GCT EcoNI 1212 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 493 GAATTC G^AATTC HaeII 29 AGCGCT [GA]GCGC^[CT] HhaI 30 GCGC GCG^C HinP1I 30 GCGC G^CGC HpaI 535 GTTAAC GTT^AAC HphI 167 TCACC GGTGA(8/7) Ksp632I 907 GAAGAG CTCTTC(1/4) MfeI 554 CAATTG C^AATTG MflI 956 AGATCT [GA]^GATC[CT] MluNI 338 TGGCCA TGG^CCA MscI 338 TGGCCA TGG^CCA MunI 554 CAATTG C^AATTG PflFI 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1032 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 755 ACCGGT A^CCGGT PleI 1169 GACTC GAGTC(4/5) PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT] SfiI 13 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 42 CCCGGG CCC^GGG TaqI 491 TCGA T^CGA TspRI 151 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 491 TCGA T^CGA Van91I 1032 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 956 AGATCT [GA]^GATC[CT] XmaCI 42 CCCGGG C^CCGGG XmaI 42 CCCGGG C^CCGGG XmnI 365 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 836 TCCGGA T^CCGGA 914 TCCGGA AflIII 1007 ACGTGT A^C[GA][CT]GT 1013 ACATGT AlwI 850 GATCC GGATC(4/5) 583 GGATC BfaI 941 CTAG C^TAG 1162 CTAG BlpI 843 GCTCAGC GC^T[ACGT]AGC 966 GCTCAGC BpmI 25 CTGGAG CTGGAG(16/14) 952 CTGGAG Bpu1102I 843 GCTCAGC GC^T[ACGT]AGC 966 GCTCAGC BseAI 836 TCCGGA T^CCGGA 914 TCCGGA BsmFI 89 GGGAC GGGAC(10/14) 655 GGGAC BspEI 836 TCCGGA T^CCGGA 914 TCCGGA BsrDI 1224 CATTGC GCAATG(2/0) 221 GCAATG BsrI 296 ACTGG ACTGG(1/-1) 1064 ACTGG BstF5I 870 CATCC GGATG(2/0) 1258 CATCC CelII 843 GCTCAGC GC^T[ACGT]AGC 966 GCTCAGC DraII 11 AGGGCCT [GA]G^G[ACGT]CC[CT] 655 GGGACCC DsaI 275 CCATGG C^C[GA][CT]GG 1037 CCATGG EcoO109I 11 AGGGCCT [GA]G^G[ACGT]CC[CT] 655 GGGACCC FauI 63 GCGGG CCCGC(4/6) 141 GCGGG FokI 870 CATCC GGATG(9/13) 1258 CATCC HincII 133 GTCAAC GT[CT]^[GA]AC 535 GTTAAC HindII 133 GTCAAC GT[CT]^[GA]AC 535 GTTAAC MaeI 941 CTAG C^TAG 1162 CTAG MroI 836 TCCGGA T^CCGGA 914 TCCGGA NcoI 275 CCATGG C^CATGG 1037 CCATGG NspI 1013 ACATGT [GA]CATG^[CT] 1055 ACATGC PstI 36 CTGCAG CTGCA^G 70 CTGCAG SacI 964 GAGCTC GAGCT^C 984 GAGCTC ScaI 158 AGTACT AGT^ACT 519 AGTACT SfaNI 200 GATGC GCATC(5/9) 486 GCATC Tsp45I 452 GTGAC ^GT[GC]AC 643 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AspHI BanII BbvI BcnI BmyI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI Bsp1286I Bsp1407I BsrGI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI SspBI StyI TfiI Tru9I TseI Tsp509I XcmI