(Linear) (Six-base) MAPSORT of: KIEE1903.seq Check: 7696 from: 1 to: 1299 KIEE1903.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1234 1299 Size: 1234 65 AfeI AGC'GCT Cuts at: 0 31 1299 Size: 31 1268 AflIII A'CryG_T Cuts at: 0 1007 1013 1299 Size: 1007 6 286 Fragments arranged by size: 1007 286 6 AgeI A'CCGG_T Cuts at: 0 755 1299 Size: 755 544 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 234 1299 Size: 234 1065 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 82 114 1299 Size: 82 32 1185 Fragments arranged by size: 1185 82 32 ApoI r'AATT_y Cuts at: 0 493 1299 Size: 493 806 AvaI C'yCGr_G Cuts at: 0 42 1299 Size: 42 1257 BanII G_rGCy'C Cuts at: 0 69 968 988 1077 1299 Size: 69 899 20 89 222 Fragments arranged by size: 899 222 89 69 20 BglI GCCn_nnn'nGGC Cuts at: 0 20 1299 Size: 20 1279 BglII A'GATC_T Cuts at: 0 956 1299 Size: 956 343 BlpI GC'TnA_GC Cuts at: 0 844 967 1299 Size: 844 123 332 Fragments arranged by size: 844 332 123 BmrI ACTGGGnnnn_n' Cuts at: 0 1074 1299 Size: 1074 225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 973 1299 Size: 46 927 326 Fragments arranged by size: 927 326 46 BsaAI yAC'GTr Cuts at: 0 123 1299 Size: 123 1176 BsaWI w'CCGG_w Cuts at: 0 755 836 914 1299 Size: 755 81 78 385 Fragments arranged by size: 755 385 81 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 689 719 1129 1159 1299 Size: 463 30 196 30 410 30 140 Fragments arranged by size: 463 410 196 140 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 715 1299 Size: 715 584 BsiHKAI G_wGCw'C Cuts at: 0 128 968 988 1299 Size: 128 840 20 311 Fragments arranged by size: 840 311 128 20 Bsp1286I G_dGCh'C Cuts at: 0 69 128 968 988 1077 1299 Size: 69 59 840 20 89 222 Fragments arranged by size: 840 222 89 69 59 20 BspEI T'CCGG_A Cuts at: 0 836 914 1299 Size: 836 78 385 Fragments arranged by size: 836 385 78 BsrBI CCG'CTC Cuts at: 0 141 1299 Size: 141 1158 BsrDI GCAATG_nn' Cuts at: 0 228 1223 1299 Size: 228 995 76 Fragments arranged by size: 995 228 76 BsrFI r'CCGG_y Cuts at: 0 755 1299 Size: 755 544 BsrGI T'GTAC_A Cuts at: 0 678 1010 1052 1299 Size: 678 332 42 247 Fragments arranged by size: 678 332 247 42 BstAPI GCAn_nnn'nTGC Cuts at: 0 152 1299 Size: 152 1147 BstYI r'GATC_y Cuts at: 0 956 1299 Size: 956 343 BtgI C'CryG_G Cuts at: 0 275 1037 1299 Size: 275 762 262 Fragments arranged by size: 762 275 262 BtsI GCAGTG_nn' Cuts at: 0 150 1299 Size: 150 1149 EaeI y'GGCC_r Cuts at: 0 338 1299 Size: 338 961 EarI CTCTTCn'nnn_ Cuts at: 0 902 1299 Size: 902 397 EcoICRI GAG'CTC Cuts at: 0 966 986 1299 Size: 966 20 313 Fragments arranged by size: 966 313 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 973 1234 1299 Size: 46 927 261 65 Fragments arranged by size: 927 261 65 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 1216 1299 Size: 1216 83 EcoO109I rG'GnC_Cy Cuts at: 0 12 656 1299 Size: 12 644 643 Fragments arranged by size: 644 643 12 EcoRI G'AATT_C Cuts at: 0 493 1299 Size: 493 806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 785 1299 Size: 785 514 HaeII r_GCGC'y Cuts at: 0 33 1299 Size: 33 1266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 388 420 866 898 1079 1111 1299 Size: 388 32 446 32 181 32 188 Fragments arranged by size: 446 388 188 181 32 32 32 HincII GTy'rAC Cuts at: 0 135 537 1299 Size: 135 402 762 Fragments arranged by size: 762 402 135 HpaI GTT'AAC Cuts at: 0 537 1299 Size: 537 762 MfeI C'AATT_G Cuts at: 0 554 1299 Size: 554 745 MscI TGG'CCA Cuts at: 0 340 1299 Size: 340 959 NcoI C'CATG_G Cuts at: 0 275 1037 1299 Size: 275 762 262 Fragments arranged by size: 762 275 262 NspI r_CATG'y Cuts at: 0 1017 1059 1299 Size: 1017 42 240 Fragments arranged by size: 1017 240 42 PciI A'CATG_T Cuts at: 0 1013 1299 Size: 1013 286 PflMI CCAn_nnn'nTGG Cuts at: 0 1038 1299 Size: 1038 261 PpuMI rG'GwC_Cy Cuts at: 0 656 1299 Size: 656 643 PstI C_TGCA'G Cuts at: 0 40 74 1299 Size: 40 34 1225 Fragments arranged by size: 1225 40 34 SacI G_AGCT'C Cuts at: 0 968 988 1299 Size: 968 20 311 Fragments arranged by size: 968 311 20 SanDI GG'GwC_CC Cuts at: 0 656 1299 Size: 656 643 ScaI AGT'ACT Cuts at: 0 160 521 1299 Size: 160 361 778 Fragments arranged by size: 778 361 160 SfcI C'TryA_G Cuts at: 0 36 70 1107 1299 Size: 36 34 1037 192 Fragments arranged by size: 1037 192 36 34 SfiI GGCCn_nnn'nGGCC Cuts at: 0 20 1299 Size: 20 1279 SmaI CCC'GGG Cuts at: 0 44 1299 Size: 44 1255 SrfI GCCC'GGGC Cuts at: 0 44 1299 Size: 44 1255 StyI C'CwwG_G Cuts at: 0 275 806 824 1037 1299 Size: 275 531 18 213 262 Fragments arranged by size: 531 275 262 213 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 371 1030 1299 Size: 371 659 269 Fragments arranged by size: 659 371 269 TatI w'GTAC_w Cuts at: 0 158 519 678 1010 1052 1127 1299 Size: 158 361 159 332 42 75 172 Fragments arranged by size: 361 332 172 159 158 75 42 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 463 495 919 951 1299 Size: 463 32 424 32 348 Fragments arranged by size: 463 424 348 32 32 Tth111I GACn'n_nGTC Cuts at: 0 880 1299 Size: 880 419 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 258 876 1230 1299 Size: 258 618 354 69 Fragments arranged by size: 618 354 258 69 XmaI C'CCGG_G Cuts at: 0 42 1299 Size: 42 1257 XmnI GAAnn'nnTTC Cuts at: 0 369 1299 Size: 369 930 Enzymes that do cut: AcuI AfeI AflIII AgeI AlfI AloI ApoI AvaI BanII BglI BglII BlpI BmrI BpmI BsaAI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MfeI MscI NcoI NspI PciI PflMI PpuMI PstI SacI SanDI ScaI SfcI SfiI SmaI SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HindIII KasI KpnI MluI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI ZraI