##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AlwI          224  GGATC                GGATC(4/5)
AspHI         216  GAGCTC               G[AT]GC[AT]^C
BanII         216  GAGCTC               G[GA]GC[CT]^C
BcnI          153  CCCGG                CC^[GC]GG
BmyI          216  GAGCTC               G[AGT]GC[ACT]^C
BpmI           64  CTGGAG               CTGGAG(16/14)
BsaBI         157  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI          67  GAGGAG               GAGGAG(10/8)
BsiHKAI       216  GAGCTC               G[AT]GC[AT]^C
BsiYI         202  CCCAACAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          202  CCCAACAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         236  GTCTC                GTCTC(1/5)
Bsp1286I      216  GAGCTC               G[AGT]GC[ACT]^C
BstF5I        156  GGATG                GGATG(2/0)
BstXI          92  CCAACTGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         225  GATC                 ^GATC
FokI          156  GGATG                GGATG(9/13)
HapII         154  CCGG                 C^CGG
HpaII         154  CCGG                 C^CGG
MamI          157  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          225  GATC                 ^GATC
MspI          154  CCGG                 C^CGG
NciI          153  CCCGG                CC^[GC]GG
NdeII         225  GATC                 ^GATC
PleI          234  GAGTC                GAGTC(4/5)
SacI          216  GAGCTC               GAGCT^C
Sau3AI        225  GATC                 ^GATC
TfiI           88  GAATC                G^A[AT]TC
TspRI         207  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI           92  CCAACTGCTTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BstNI          63  CCTGG                CC^[AT]GG
              221  CCTGG               
DdeI          130  CTGAG                C^T[ACGT]AG
              232  CTGAG               
EcoRII         63  CCTGG                ^CC[AT]GG
              221  CCTGG               
HinfI          88  GAATC                G^A[ACGT]TC
              234  GAGTC               
MnlI           67  GAGG                 CCTC(7/6)
              144  GAGG                
MvaI           63  CCTGG                CC^[AT]GG
              221  CCTGG               
NlaIII         40  CATG                 CATG^
              249  CATG                
SfaNI         157  GATGC                GCATC(5/9)
                4  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        BssKI       ScrFI