Restriction map for
pFN21AE3818
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AgeI Aor51HI ApaI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BbsI BciVI BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoO65I EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1399 GACGTC GACGT^C
Acc65I 518 GGTACC G^GTACC
AccI 3328 GTAGAC GT^[AC][GT]AC
AflII 1287 CTTAAG C^TTAAG
AflIII 645 ACGTGT A^C[GA][CT]GT
AhdI 3280 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 518 GGTACC G^GTACC
AspEI 3280 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2442 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3478 CCCGGG C^[CT]CG[GA]G
BcgI 2129 CGAATCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 156 TGATCA T^GATCA
BfrI 1287 CTTAAG C^TTAAG
BglII 1224 AGATCT A^GATCT
BlpI 558 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 558 GCTCAGC GC^T[ACGT]AGC
BsiEI 93 CGGCCG CG[GA][CT]^CG
BsmI 1123 GAATGC GAATGC(1/-1)
BsoBI 3478 CCCGGG C^[CT]CG[GA]G
BspHI 1967 TCATGA T^CATGA
BsrFI 130 ACCGGC [GA]^CCGG[CT]
BstAPI 1783 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 167 CCAGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 558 GCTCAGC GC^T[ACGT]AGC
Cfr10I 130 ACCGGC [GA]^CCGG[CT]
EagI 93 CGGCCG C^GGCCG
Eam1105I 3280 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 93 CGGCCG C^GGCCG
Eco52I 93 CGGCCG C^GGCCG
EcoNI 1470 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 982 GAATTC G^AATTC
EcoRV 1502 GATATC GAT^ATC
FbaI 156 TGATCA T^GATCA
KpnI 518 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
PflFI 2442 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2713 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 527 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1967 TCATGA T^CATGA
SacI 285 GAGCTC GAGCT^C
SacII 57 CCGCGG CCGC^GG
SbfI 3438 CCTGCAGG CCTGCA^GG
ScaI 1193 AGTACT AGT^ACT
SmaI 3478 CCCGGG CCC^GGG
Sse8387I 3438 CCTGCAGG CCTGCA^GG
StuI 1844 AGGCCT AGG^CCT
Tth111I 2442 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2713 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3478 CCCGGG C^CCGGG
XmaI 3478 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 19 CGCG CG^CG
58 CGCG
AcyI 913 GGCGTC G[GA]^CG[CT]C
1399 GACGTC
Alw44I 1838 GTGCAC G^TGCAC
3293 GTGCAC
ApaLI 1838 GTGCAC G^TGCAC
3293 GTGCAC
Asp700I 1206 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2865 GAATAAATTC
BalI 1166 TGGCCA TGG^CCA
3173 TGGCCA
BamHI 62 GGATCC G^GATCC
1855 GGATCC
BbvCI 49 CCTCAGC CCTCAGC(-5/-2)
1657 GCTGAGG
BglI 2730 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3166 GCCAAAATGGC
BsaHI 913 GGCGTC G[GA]^CG[CT]C
1399 GACGTC
BsaI 127 GAGACC GGTCTC(1/5)
2538 GAGACC
Bsp1407I 732 TGTACA T^GTACA
3516 TGTACA
BsrDI 1186 GCAATG GCAATG(2/0)
2392 GCAATG
BsrGI 732 TGTACA T^GTACA
3516 TGTACA
BstUI 19 CGCG CG^CG
58 CGCG
CfoI 117 GCGC GCG^C
3083 GCGC
DraIII 536 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2785 CACAAAGTG
HgaI 20 GCGTC GACGC(5/10)
914 GCGTC
HhaI 117 GCGC GCG^C
3083 GCGC
Hin1I 913 GGCGTC G[GA]^CG[CT]C
1399 GACGTC
HinP1I 117 GCGC G^CGC
3083 GCGC
HindIII 221 AAGCTT A^AGCTT
1610 AAGCTT
MluNI 1166 TGGCCA TGG^CCA
3173 TGGCCA
MscI 1166 TGGCCA TGG^CCA
3173 TGGCCA
MvnI 19 CGCG CG^CG
58 CGCG
NcoI 522 CCATGG C^CATGG
968 CCATGG
PpuMI 104 GGGACCC [GA]G^G[AT]CC[CT]
2621 AGGACCC
SapI 3010 GAAGAGC GCTCTTC(1/4)
2575 GCTCTTC
SmlI 1287 CTTAAG C^T[CT][GA]AG
3131 CTCAAG
SphI 1558 GCATGC GCATG^C
2088 GCATGC
SspBI 732 TGTACA T^GTACA
3516 TGTACA
TaqI 1506 TCGA T^CGA
1872 TCGA
TfiI 2028 GAATC G^A[AT]TC
2130 GAATC
TthHB8I 1506 TCGA T^CGA
1872 TCGA
XmnI 1206 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2865 GAATAAATTC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII