(Circular) (Six-base) MAPSORT of: pFN21AE3818.seq Check: 4159 from: 1 to: 8251 pFN21AE3818 8251 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2005 2005 Size: 8251 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3408 6061 278 Size: 53 83 186 936 1872 2653 2468 Fragments arranged by size: 2653 2468 1872 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5334 5594 1937 Size: 3397 260 4594 Fragments arranged by size: 4594 3397 260 Acc65I G'GTAC_C Cuts at: 2523 5572 2523 Size: 3049 5202 AclI AA'CG_TT Cuts at: 1563 6378 6751 1563 Size: 4815 373 3063 Fragments arranged by size: 4815 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 3672 3770 3946 5448 6314 7698 7966 Size: 1069 971 98 176 1502 866 1384 268 Cuts at: 7966 1632 Size: 1917 Fragments arranged by size: 1917 1502 1384 1069 971 866 268 176 98 AflII C'TTAA_G Cuts at: 820 1017 3292 820 Size: 197 2275 5779 Fragments arranged by size: 5779 2275 197 AflIII A'CryG_T Cuts at: 1203 2650 7150 8127 1203 Size: 1447 4500 977 1327 Fragments arranged by size: 4500 1447 1327 977 AgeI A'CCGG_T Cuts at: 7082 7082 Size: 8251 AhdI GACnn_n'nnGTC Cuts at: 5290 6980 5290 Size: 1690 6561 AleI CACnn'nnGTG Cuts at: 1166 2495 2797 4150 1166 Size: 1329 302 1353 5267 Fragments arranged by size: 5267 1353 1329 302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8251 AlwNI CAG_nnn'CTG Cuts at: 2759 2948 4416 5471 5474 5994 7566 2759 Size: 189 1468 1055 3 520 1572 3444 Fragments arranged by size: 3444 1572 1468 1055 520 189 3 ApaLI G'TGCA_C Cuts at: 3843 5298 6308 7464 3843 Size: 1455 1010 1156 4630 Fragments arranged by size: 4630 1455 1156 1010 ApoI r'AATT_y Cuts at: 1493 2987 3106 3214 3341 3805 4874 5402 5560 Size: 1494 119 108 127 464 1069 528 158 Cuts at: 5560 5786 5938 8047 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1494 1069 528 464 226 158 152 127 119 108 AseI AT'TA_AT Cuts at: 160 1032 6805 160 Size: 872 5773 1606 Fragments arranged by size: 5773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8251 AvaI C'yCGr_G Cuts at: 1870 1943 5483 5576 1870 Size: 73 3540 93 4545 Fragments arranged by size: 4545 3540 93 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2639 2672 5187 5220 2639 Size: 33 2515 33 5670 Fragments arranged by size: 5670 2515 33 33 BamHI G'GATC_C Cuts at: 2067 3860 5581 5948 2067 Size: 1793 1721 367 4370 Fragments arranged by size: 4370 1793 1721 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2473 2523 4141 5572 7027 Size: 325 217 627 686 50 1618 1431 1455 Cuts at: 7027 618 Size: 1842 Fragments arranged by size: 1842 1618 1455 1431 686 627 325 217 50 BanII G_rGCy'C Cuts at: 1391 2046 2294 3581 5570 1391 Size: 655 248 1287 1989 4072 Fragments arranged by size: 4072 1989 1287 655 248 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7280 BbvCI CC'TCA_GC Cuts at: 2055 3663 2055 Size: 1608 6643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 6442 6476 1903 Size: 34 2186 34 2285 34 3678 Fragments arranged by size: 3678 2285 2186 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6143 7353 6143 Size: 1210 7041 BclI T'GATC_A Cuts at: 1548 2161 1548 Size: 613 7638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 5177 6862 Size: 107 122 71 631 605 3069 436 1685 Cuts at: 6862 136 Size: 1525 Fragments arranged by size: 3069 1685 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 3229 8246 3229 Size: 5017 3234 BlpI GC'TnA_GC Cuts at: 2564 5668 2564 Size: 3104 5147 Bme1580I G_kGCm'C Cuts at: 1790 3790 3847 4696 4954 5097 5302 5319 5484 Size: 2000 57 849 258 143 205 17 165 Cuts at: 5484 6312 7468 1790 Size: 828 1156 2573 Fragments arranged by size: 2573 2000 1156 849 828 258 205 165 143 57 17 BmgBI CAC'GTC Cuts at: 1318 2651 4840 1318 Size: 1333 2189 4729 Fragments arranged by size: 4729 2189 1333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2489 4295 5982 Size: 416 53 83 399 24 1072 1806 1687 Cuts at: 5982 6940 442 Size: 958 1753 Fragments arranged by size: 1806 1753 1687 1072 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8251 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4005 5257 4005 Size: 1252 6999 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2229 3330 3354 3625 4418 4617 4671 Size: 791 199 1101 24 271 793 199 54 Cuts at: 4671 4731 4734 4815 4932 6911 1239 Size: 60 3 81 117 1979 2579 Fragments arranged by size: 2579 1979 1101 793 791 271 199 199 117 81 60 54 24 3 Bpu10I CC'TnA_GC Cuts at: 2055 2636 3663 5365 2055 Size: 581 1027 1702 4941 Fragments arranged by size: 4941 1702 1027 581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5121 6376 7241 7539 5121 Size: 1255 865 298 5833 Fragments arranged by size: 5833 1255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 4537 6914 882 Size: 617 627 2411 2377 2219 Fragments arranged by size: 2411 2377 2219 627 617 BsaAI yAC'GTr Cuts at: 493 1204 6042 493 Size: 711 4838 2702 Fragments arranged by size: 4838 2702 711 BsaBI GATnn'nnATC Cuts at: 8245 8245 Size: 8251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2919 3405 6058 6440 Size: 53 83 186 936 1386 486 2653 382 Cuts at: 6440 7075 275 Size: 635 1451 Fragments arranged by size: 2653 1451 1386 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6684 7082 7356 7503 7914 6684 Size: 398 274 147 411 7021 Fragments arranged by size: 7021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 1178 Size: 30 165 30 1329 30 6667 Fragments arranged by size: 6667 1329 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3676 3680 4584 1186 Size: 2490 4 904 4853 Fragments arranged by size: 4853 2490 904 4 BseYI C'CCAG_C Cuts at: 2154 2469 2788 3865 3899 4122 4226 5111 7454 Size: 315 319 1077 34 223 104 885 2343 Cuts at: 7454 7950 2154 Size: 496 2455 Fragments arranged by size: 2455 2343 1077 885 496 319 315 223 104 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2103 2568 4862 5564 1163 Size: 573 367 465 2294 702 3850 Fragments arranged by size: 3850 2294 702 573 465 367 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 5701 6462 7490 1540 Size: 275 186 100 3600 761 1028 2301 Fragments arranged by size: 3600 2301 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 2294 3847 5302 6312 6397 7468 2294 Size: 1553 1455 1010 85 1071 3077 Fragments arranged by size: 3077 1553 1455 1071 1010 85 BsmI GAATG_Cn' Cuts at: 3134 5762 5855 3134 Size: 2628 93 5530 Fragments arranged by size: 5530 2628 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2294 3581 3790 3847 4696 4954 Size: 399 256 248 1287 209 57 849 258 Cuts at: 4954 5097 5302 5319 5484 5570 6312 6397 7468 Size: 143 205 17 165 86 742 85 1071 Cuts at: 7468 1391 Size: 2174 Fragments arranged by size: 2174 1287 1071 849 742 399 258 256 248 209 205 165 143 86 85 57 17 BspEI T'CCGG_A Cuts at: 7914 7914 Size: 8251 BspHI T'CATG_A Cuts at: 3972 6140 7870 3972 Size: 2168 1730 4353 Fragments arranged by size: 4353 2168 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 3717 4289 5183 5606 844 Size: 1161 1712 572 894 423 3489 Fragments arranged by size: 3489 1712 1161 894 572 423 BsrBI CCG'CTC Cuts at: 1394 1823 6138 1394 Size: 429 4315 3507 Fragments arranged by size: 4315 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3198 4404 6746 6920 65 Size: 1183 1950 1206 2342 174 1396 Fragments arranged by size: 2342 1950 1396 1206 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 6895 7082 1700 Size: 110 325 4760 187 2869 Fragments arranged by size: 4760 2869 325 187 110 BsrGI T'GTAC_A Cuts at: 96 2737 5521 96 Size: 2641 2784 2826 Fragments arranged by size: 2826 2784 2641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8251 BssSI C'ACGA_G Cuts at: 6311 7323 6311 Size: 1012 7239 BstAPI GCAn_nnn'nTGC Cuts at: 794 3794 794 Size: 3000 5251 BstBI TT'CG_AA Cuts at: 7055 7055 Size: 8251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8251 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2179 Size: 8251 BstYI r'GATC_y Cuts at: 1972 2067 3229 3860 4606 5581 5948 6333 6350 Size: 95 1162 631 746 975 367 385 17 Cuts at: 6350 7802 7888 7900 8246 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1162 975 746 631 385 367 346 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3525 3540 4369 3525 Size: 15 829 7407 Fragments arranged by size: 7407 829 15 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2527 2973 513 Size: 552 939 58 465 446 5791 Fragments arranged by size: 5791 939 552 465 446 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7512 Fragments arranged by size: 7512 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2551 2676 3869 4221 4222 5097 5767 6561 Size: 465 125 1193 352 1 875 670 794 Cuts at: 6561 6581 2086 Size: 20 3756 Fragments arranged by size: 3756 1193 875 794 670 465 352 125 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8216 DraI TTT'AAA Cuts at: 5555 5555 Size: 8251 DraIII CAC_nnn'GTG Cuts at: 2546 4795 2546 Size: 2249 6002 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7258 809 Size: 507 5942 1802 Fragments arranged by size: 5942 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3171 5178 Size: 54 1425 50 275 18 268 1073 2007 Cuts at: 5178 5698 6587 8216 8 Size: 520 889 1629 43 Fragments arranged by size: 2007 1629 1425 1073 889 520 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2098 5698 1537 Size: 275 286 3600 4090 Fragments arranged by size: 4090 3600 286 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 3135 3648 3974 4465 4467 4587 5010 Size: 989 793 513 326 491 2 120 423 Cuts at: 5010 6181 1353 Size: 1171 3423 Fragments arranged by size: 3423 1171 989 793 513 491 423 326 120 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6838 7212 7358 387 Size: 1064 138 5249 374 146 1280 Fragments arranged by size: 5249 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2292 2292 Size: 8251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2229 2701 3330 3354 3625 3672 Size: 393 398 199 472 629 24 271 47 Cuts at: 3672 3770 3946 4418 4617 4671 4731 4734 4815 Size: 98 176 472 199 54 60 3 81 Cuts at: 4815 4932 5448 6314 6911 7698 7966 1239 Size: 117 516 866 597 787 268 1524 Fragments arranged by size: 1524 866 787 629 597 516 472 472 398 393 271 268 199 199 176 117 98 81 60 54 47 24 3 EcoNI CCTnn'n_nnAGG Cuts at: 3479 3479 Size: 8251 EcoO109I rG'GnC_Cy Cuts at: 2091 2110 3591 4627 5266 2091 Size: 19 1481 1036 639 5076 Fragments arranged by size: 5076 1481 1036 639 19 EcoRI G'AATT_C Cuts at: 2987 5560 5938 2987 Size: 2573 378 5300 Fragments arranged by size: 5300 2573 378 EcoRV GAT'ATC Cuts at: 3509 3509 Size: 8251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2320 3973 2320 Size: 1653 6598 FspI TGC'GCA Cuts at: 6757 6757 Size: 8251 HaeII r_GCGC'y Cuts at: 1678 7398 1678 Size: 5720 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3578 3610 4091 4123 5276 Size: 32 135 32 2006 32 481 32 1153 Cuts at: 5276 5308 5348 5380 6892 6924 6966 6998 1373 Size: 32 40 32 1512 32 42 32 2626 Fragments arranged by size: 2626 2006 1512 1153 481 135 42 40 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5595 5841 669 Size: 988 281 3657 246 3079 Fragments arranged by size: 3657 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2226 3615 748 Size: 1478 1389 5384 Fragments arranged by size: 5384 1478 1389 HpaI GTT'AAC Cuts at: 5841 5841 Size: 8251 KpnI G_GTAC'C Cuts at: 2527 5576 2527 Size: 3049 5202 MfeI C'AATT_G Cuts at: 5850 5850 Size: 8251 MluI A'CGCG_T Cuts at: 8127 8127 Size: 8251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7365 7549 1305 Size: 6060 184 2007 Fragments arranged by size: 6060 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3173 5180 10 Size: 54 1425 343 1341 2007 3081 Fragments arranged by size: 3081 2007 1425 1341 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2797 4098 4150 6209 6568 6727 Size: 648 1329 302 1301 52 2059 359 159 Cuts at: 6727 518 Size: 2042 Fragments arranged by size: 2059 2042 1329 1301 648 359 302 159 52 MspA1I CmG'CkG Cuts at: 1606 2064 2175 2552 2623 4438 4661 4835 5231 Size: 458 111 377 71 1815 223 174 396 Cuts at: 5231 5329 5350 5471 5667 6344 7492 7737 1606 Size: 98 21 121 196 677 1148 245 2120 Fragments arranged by size: 2120 1815 1148 677 458 396 377 245 223 196 174 121 111 98 71 21 NcoI C'CATG_G Cuts at: 513 1065 2004 2527 2973 513 Size: 552 939 523 446 5791 Fragments arranged by size: 5791 939 552 523 446 NdeI CA'TA_TG Cuts at: 387 387 Size: 8251 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8251 NotI GC'GGCC_GC Cuts at: 5698 5698 Size: 8251 NspI r_CATG'y Cuts at: 1132 1229 3097 3567 3933 4097 4501 5609 7154 Size: 97 1868 470 366 164 404 1108 1545 Cuts at: 7154 1132 Size: 2229 Fragments arranged by size: 2229 1868 1545 1108 470 404 366 164 97 PasI CC'CwG_GG Cuts at: 1348 2615 1348 Size: 1267 6984 PciI A'CATG_T Cuts at: 7150 7150 Size: 8251 PflMI CCAn_nnn'nTGG Cuts at: 1108 4724 1108 Size: 3616 4635 PmeI GTTT'AAAC Cuts at: 5555 5555 Size: 8251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6320 6352 7833 7865 6320 Size: 32 1481 32 6706 Fragments arranged by size: 6706 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 4627 2110 Size: 2517 5734 PshAI GACnn'nnGTC Cuts at: 1613 2536 1613 Size: 923 7328 PsiI TTA'TAA Cuts at: 5821 5821 Size: 8251 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2851 2883 2851 Size: 32 8219 PstI C_TGCA'G Cuts at: 830 2683 3007 3154 4986 5038 5448 5603 830 Size: 1853 324 147 1832 52 410 155 3478 Fragments arranged by size: 3478 1853 1832 410 324 155 147 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8251 PvuII CAG'CTG Cuts at: 2175 2552 2623 4438 4661 4835 5329 5350 5471 Size: 377 71 1815 223 174 494 21 121 Cuts at: 5471 2175 Size: 4955 Fragments arranged by size: 4955 1815 494 377 223 174 121 71 21 SacI G_AGCT'C Cuts at: 2294 2294 Size: 8251 SacII CC_GC'GG Cuts at: 2065 2065 Size: 8251 SalI G'TCGA_C Cuts at: 1936 5593 1936 Size: 3657 4594 SanDI GG'GwC_CC Cuts at: 2110 2110 Size: 8251 SapI GCTCTTCn'nnn_ Cuts at: 4587 5010 4587 Size: 423 7828 SbfI CC_TGCA'GG Cuts at: 5448 5603 5448 Size: 155 8096 ScaI AGT'ACT Cuts at: 3200 6499 8211 3200 Size: 3299 1712 3240 Fragments arranged by size: 3299 3240 1712 SfcI C'TryA_G Cuts at: 826 1045 2662 2679 2811 3003 3150 4088 4982 Size: 219 1617 17 132 192 147 938 894 Cuts at: 4982 5010 5034 5444 5453 5599 6734 7415 7606 Size: 28 24 410 9 146 1135 681 191 Cuts at: 7606 826 Size: 1471 Fragments arranged by size: 1617 1471 1135 938 894 681 410 219 192 191 147 146 132 28 24 17 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8251 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8251 SmaI CCC'GGG Cuts at: 1872 5485 5578 1872 Size: 3613 93 4545 Fragments arranged by size: 4545 3613 93 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 5136 6355 7256 7518 820 Size: 197 926 1349 1844 1219 901 262 1553 Fragments arranged by size: 1844 1553 1349 1219 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6042 493 Size: 5549 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8251 SphI G_CATG'C Cuts at: 1132 3567 4097 5609 1132 Size: 2435 530 1512 3774 Fragments arranged by size: 3774 2435 1512 530 SspI AAT'ATT Cuts at: 5 52 6175 5 Size: 47 6123 2081 Fragments arranged by size: 6123 2081 47 StuI AGG'CCT Cuts at: 1517 3851 1517 Size: 2334 5917 StyI C'CwwG_G Cuts at: 513 1065 2004 2295 2413 2527 2724 2973 4340 Size: 552 939 291 118 114 197 249 1367 Cuts at: 4340 4668 4755 5690 513 Size: 328 87 935 3074 Fragments arranged by size: 3074 1367 939 935 552 328 291 249 197 118 114 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 2461 2809 3913 4162 4224 4828 Size: 1255 588 293 348 1104 249 62 604 Cuts at: 4828 4938 6278 6295 6448 6633 7078 325 Size: 110 1340 17 153 185 445 1498 Fragments arranged by size: 1498 1340 1255 1104 604 588 445 348 293 249 185 153 110 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2737 3056 3198 Size: 274 80 33 51 1443 760 319 142 Cuts at: 3198 5521 6497 8209 96 Size: 2323 976 1712 138 Fragments arranged by size: 2323 1712 1443 976 760 319 274 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2732 2764 4567 4599 8211 8243 2732 Size: 32 1803 32 3612 32 2740 Fragments arranged by size: 3612 2740 1803 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4450 1316 Size: 3134 5117 XbaI T'CTAG_A Cuts at: 5587 5587 Size: 8251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3907 4435 5326 3907 Size: 528 891 6832 Fragments arranged by size: 6832 891 528 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8251 XmaI C'CCGG_G Cuts at: 1870 5483 5576 1870 Size: 3613 93 4545 Fragments arranged by size: 4545 3613 93 XmnI GAAnn'nnTTC Cuts at: 3215 4874 6380 3215 Size: 1659 1506 5086 Fragments arranged by size: 5086 1659 1506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3406 6059 276 Size: 53 83 186 936 1872 2653 2468 Fragments arranged by size: 2653 2468 1872 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SexAI SfiI SfoI SrfI SwaI