Restriction map for
pFN21AE3818
(Linear) (Six-base) MAPSORT of: KIEE3818.seq Check: 3396 from: 1 to: 3546
KIEE3818.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AatII G_ACGT'C
Cuts at: 0 1403 3546
Size: 1403 2143
AccI GT'mk_AC
Cuts at: 0 3329 3546
Size: 3329 217
Acc65I G'GTAC_C
Cuts at: 0 518 3546
Size: 518 3028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1667 1765 1941 3443 3546
Size: 696 971 98 176 1502 103
Fragments arranged by size:
1502 971 696 176 103 98
AflII C'TTAA_G
Cuts at: 0 1287 3546
Size: 1287 2259
AflIII A'CryG_T
Cuts at: 0 645 3546
Size: 645 2901
AhdI GACnn_n'nnGTC
Cuts at: 0 3285 3546
Size: 3285 261
AleI CACnn'nnGTG
Cuts at: 0 490 792 2145 3546
Size: 490 302 1353 1401
Fragments arranged by size:
1401 1353 490 302
AlwNI CAG_nnn'CTG
Cuts at: 0 754 943 2411 3466 3469 3546
Size: 754 189 1468 1055 3 77
Fragments arranged by size:
1468 1055 754 189 77 3
ApaLI G'TGCA_C
Cuts at: 0 1838 3293 3546
Size: 1838 1455 253
Fragments arranged by size:
1838 1455 253
ApoI r'AATT_y
Cuts at: 0 982 1101 1209 1336 1800 2869 3397 3546
Size: 982 119 108 127 464 1069 528 149
Fragments arranged by size:
1069 982 528 464 149 127 119 108
AvaI C'yCGr_G
Cuts at: 0 3478 3546
Size: 3478 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 634 667 3182 3215 3546
Size: 634 33 2515 33 331
Fragments arranged by size:
2515 634 331 33 33
BamHI G'GATC_C
Cuts at: 0 62 1855 3546
Size: 62 1793 1691
Fragments arranged by size:
1793 1691 62
BanI G'GyrC_C
Cuts at: 0 468 518 2136 3546
Size: 468 50 1618 1410
Fragments arranged by size:
1618 1410 468 50
BanII G_rGCy'C
Cuts at: 0 41 289 1576 3546
Size: 41 248 1287 1970
Fragments arranged by size:
1970 1287 248 41
BbvCI CC'TCA_GC
Cuts at: 0 50 1658 3546
Size: 50 1608 1888
Fragments arranged by size:
1888 1608 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2118 2152 3546
Size: 2118 34 1394
Fragments arranged by size:
2118 1394 34
BclI T'GATC_A
Cuts at: 0 156 3546
Size: 156 3390
BglI GCCn_nnn'nGGC
Cuts at: 0 2736 3172 3546
Size: 2736 436 374
Fragments arranged by size:
2736 436 374
BglII A'GATC_T
Cuts at: 0 1224 3546
Size: 1224 2322
BlpI GC'TnA_GC
Cuts at: 0 559 3546
Size: 559 2987
Bme1580I G_kGCm'C
Cuts at: 0 1785 1842 2691 2949 3092 3297 3314 3479
Size: 1785 57 849 258 143 205 17 165
Cuts at: 3479 3546
Size: 67
Fragments arranged by size:
1785 849 258 205 165 143 67 57
17
BmgBI CAC'GTC
Cuts at: 0 646 2835 3546
Size: 646 2189 711
Fragments arranged by size:
2189 711 646
BmrI ACTGGGnnnn_n'
Cuts at: 0 484 2290 3546
Size: 484 1806 1256
Fragments arranged by size:
1806 1256 484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2000 3252 3546
Size: 2000 1252 294
Fragments arranged by size:
2000 1252 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 224 1325 1349 1620 2413 2612 2666
Size: 25 199 1101 24 271 793 199 54
Cuts at: 2666 2726 2729 2810 2927 3546
Size: 60 3 81 117 619
Fragments arranged by size:
1101 793 619 271 199 199 117 81
60 54 25 24 3
Bpu10I CC'TnA_GC
Cuts at: 0 50 631 1658 3360 3546
Size: 50 581 1027 1702 186
Fragments arranged by size:
1702 1027 581 186 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3116 3546
Size: 3116 430
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 2532 3546
Size: 121 2411 1014
Fragments arranged by size:
2411 1014 121
BsaHI Gr'CG_yC
Cuts at: 0 914 1400 3546
Size: 914 486 2146
Fragments arranged by size:
2146 914 486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 3546
Size: 727 30 2789
Fragments arranged by size:
2789 727 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1671 1675 2579 3546
Size: 1671 4 904 967
Fragments arranged by size:
1671 967 904 4
BseYI C'CCAG_C
Cuts at: 0 149 464 783 1860 1894 2117 2221 3106
Size: 149 315 319 1077 34 223 104 885
Cuts at: 3106 3546
Size: 440
Fragments arranged by size:
1077 885 440 319 315 223 149 104
34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 563 2857 3546
Size: 98 465 2294 689
Fragments arranged by size:
2294 689 465 98
BsiEI CG_ry'CG
Cuts at: 0 96 3546
Size: 96 3450
BsiHKAI G_wGCw'C
Cuts at: 0 289 1842 3297 3546
Size: 289 1553 1455 249
Fragments arranged by size:
1553 1455 289 249
BsmI GAATG_Cn'
Cuts at: 0 1129 3546
Size: 1129 2417
Bsp1286I G_dGCh'C
Cuts at: 0 41 289 1576 1785 1842 2691 2949 3092
Size: 41 248 1287 209 57 849 258 143
Cuts at: 3092 3297 3314 3479 3546
Size: 205 17 165 67
Fragments arranged by size:
1287 849 258 248 209 205 165 143
67 57 41 17
BspHI T'CATG_A
Cuts at: 0 1967 3546
Size: 1967 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1712 2284 3178 3546
Size: 1712 572 894 368
Fragments arranged by size:
1712 894 572 368
BsrDI GCAATG_nn'
Cuts at: 0 1193 2399 3546
Size: 1193 1206 1147
Fragments arranged by size:
1206 1193 1147
BsrFI r'CCGG_y
Cuts at: 0 130 3546
Size: 130 3416
BsrGI T'GTAC_A
Cuts at: 0 732 3516 3546
Size: 732 2784 30
Fragments arranged by size:
2784 732 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1789 3546
Size: 1789 1757
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 3546
Size: 174 3372
BstYI r'GATC_y
Cuts at: 0 62 1224 1855 2601 3546
Size: 62 1162 631 746 945
Fragments arranged by size:
1162 945 746 631 62
Bsu36I CC'TnA_GG
Cuts at: 0 1520 1535 2364 3546
Size: 1520 15 829 1182
Fragments arranged by size:
1520 1182 829 15
BtgI C'CryG_G
Cuts at: 0 57 522 968 3546
Size: 57 465 446 2578
Fragments arranged by size:
2578 465 446 57
BtsI GCAGTG_nn'
Cuts at: 0 81 546 671 1864 2216 2217 3092 3546
Size: 81 465 125 1193 352 1 875 454
Fragments arranged by size:
1193 875 465 454 352 125 81 1
DraIII CAC_nnn'GTG
Cuts at: 0 541 2790 3546
Size: 541 2249 756
Fragments arranged by size:
2249 756 541
EaeI y'GGCC_r
Cuts at: 0 93 1166 3173 3546
Size: 93 1073 2007 373
Fragments arranged by size:
2007 1073 373 93
EagI C'GGCC_G
Cuts at: 0 93 3546
Size: 93 3453
EarI CTCTTCn'nnn_
Cuts at: 0 337 1130 1643 1969 2460 2462 2582 3005
Size: 337 793 513 326 491 2 120 423
Cuts at: 3005 3546
Size: 541
Fragments arranged by size:
793 541 513 491 423 337 326 120
2
EcoICRI GAG'CTC
Cuts at: 0 287 3546
Size: 287 3259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 224 696 1325 1349 1620 1667 1765
Size: 25 199 472 629 24 271 47 98
Cuts at: 1765 1941 2413 2612 2666 2726 2729 2810 2927
Size: 176 472 199 54 60 3 81 117
Cuts at: 2927 3443 3546
Size: 516 103
Fragments arranged by size:
629 516 472 472 271 199 199 176
117 103 98 81 60 54 47 25
24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1474 3546
Size: 1474 2072
EcoO109I rG'GnC_Cy
Cuts at: 0 86 105 1586 2622 3261 3546
Size: 86 19 1481 1036 639 285
Fragments arranged by size:
1481 1036 639 285 86 19
EcoRI G'AATT_C
Cuts at: 0 982 3546
Size: 982 2564
EcoRV GAT'ATC
Cuts at: 0 1504 3546
Size: 1504 2042
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 1968 3546
Size: 315 1653 1578
Fragments arranged by size:
1653 1578 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1573 1605 2086 2118 3271 3303 3343 3375
Size: 1573 32 481 32 1153 32 40 32
Cuts at: 3375 3546
Size: 171
Fragments arranged by size:
1573 1153 481 171 40 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 221 1610 3546
Size: 221 1389 1936
Fragments arranged by size:
1936 1389 221
KpnI G_GTAC'C
Cuts at: 0 522 3546
Size: 522 3024
MscI TGG'CCA
Cuts at: 0 1168 3175 3546
Size: 1168 2007 371
Fragments arranged by size:
2007 1168 371
MslI CAynn'nnrTG
Cuts at: 0 490 792 2093 2145 3546
Size: 490 302 1301 52 1401
Fragments arranged by size:
1401 1301 490 302 52
MspA1I CmG'CkG
Cuts at: 0 59 170 547 618 2433 2656 2830 3226
Size: 59 111 377 71 1815 223 174 396
Cuts at: 3226 3324 3345 3466 3546
Size: 98 21 121 80
Fragments arranged by size:
1815 396 377 223 174 121 111 98
80 71 59 21
NcoI C'CATG_G
Cuts at: 0 522 968 3546
Size: 522 446 2578
Fragments arranged by size:
2578 522 446
NspI r_CATG'y
Cuts at: 0 1092 1562 1928 2092 2496 3546
Size: 1092 470 366 164 404 1050
Fragments arranged by size:
1092 1050 470 404 366 164
PasI CC'CwG_GG
Cuts at: 0 610 3546
Size: 610 2936
PflMI CCAn_nnn'nTGG
Cuts at: 0 2719 3546
Size: 2719 827
PpuMI rG'GwC_Cy
Cuts at: 0 105 2622 3546
Size: 105 2517 924
Fragments arranged by size:
2517 924 105
PshAI GACnn'nnGTC
Cuts at: 0 531 3546
Size: 531 3015
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 846 878 3546
Size: 846 32 2668
Fragments arranged by size:
2668 846 32
PstI C_TGCA'G
Cuts at: 0 678 1002 1149 2981 3033 3443 3546
Size: 678 324 147 1832 52 410 103
Fragments arranged by size:
1832 678 410 324 147 103 52
PvuII CAG'CTG
Cuts at: 0 170 547 618 2433 2656 2830 3324 3345
Size: 170 377 71 1815 223 174 494 21
Cuts at: 3345 3466 3546
Size: 121 80
Fragments arranged by size:
1815 494 377 223 174 170 121 80
71 21
SacI G_AGCT'C
Cuts at: 0 289 3546
Size: 289 3257
SacII CC_GC'GG
Cuts at: 0 60 3546
Size: 60 3486
SanDI GG'GwC_CC
Cuts at: 0 105 3546
Size: 105 3441
SapI GCTCTTCn'nnn_
Cuts at: 0 2582 3005 3546
Size: 2582 423 541
Fragments arranged by size:
2582 541 423
SbfI CC_TGCA'GG
Cuts at: 0 3443 3546
Size: 3443 103
ScaI AGT'ACT
Cuts at: 0 1195 3546
Size: 1195 2351
SfcI C'TryA_G
Cuts at: 0 657 674 806 998 1145 2083 2977 3005
Size: 657 17 132 192 147 938 894 28
Cuts at: 3005 3029 3439 3448 3546
Size: 24 410 9 98
Fragments arranged by size:
938 894 657 410 192 147 132 98
28 24 17 9
SmaI CCC'GGG
Cuts at: 0 3480 3546
Size: 3480 66
SmlI C'TyrA_G
Cuts at: 0 1287 3131 3546
Size: 1287 1844 415
Fragments arranged by size:
1844 1287 415
SphI G_CATG'C
Cuts at: 0 1562 2092 3546
Size: 1562 530 1454
Fragments arranged by size:
1562 1454 530
StuI AGG'CCT
Cuts at: 0 1846 3546
Size: 1846 1700
StyI C'CwwG_G
Cuts at: 0 290 408 522 719 968 2335 2663 2750
Size: 290 118 114 197 249 1367 328 87
Cuts at: 2750 3546
Size: 796
Fragments arranged by size:
1367 796 328 290 249 197 118 114
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 456 804 1908 2157 2219 2823 2933
Size: 163 293 348 1104 249 62 604 110
Cuts at: 2933 3546
Size: 613
Fragments arranged by size:
1104 613 604 348 293 249 163 110
62
TatI w'GTAC_w
Cuts at: 0 732 1051 1193 3516 3546
Size: 732 319 142 2323 30
Fragments arranged by size:
2323 732 319 142 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 2562 2594 3546
Size: 727 32 1803 32 952
Fragments arranged by size:
1803 952 727 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2445 3546
Size: 2445 1101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1902 2430 3321 3546
Size: 1902 528 891 225
Fragments arranged by size:
1902 891 528 225
XmaI C'CCGG_G
Cuts at: 0 3478 3546
Size: 3478 68
XmnI GAAnn'nnTTC
Cuts at: 0 1210 2869 3546
Size: 1210 1659 677
Fragments arranged by size:
1659 1210 677
ZraI GAC'GTC
Cuts at: 0 1401 3546
Size: 1401 2145
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AhdI AleI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII KpnI
MscI MslI MspA1I NcoI NspI PasI PflMI PpuMI
PshAI PsrI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AlfI AloI ApaI AscI
AseI AsiSI AvrII BbeI BbsI BciVI BfrBI BmtI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BsrBI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EciI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SalI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI