################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 1005 GTGCAC G^TGCAC ApaLI 1005 GTGCAC G^TGCAC AvaI 869 CCCGGG C^[CT]CG[GA]G AvrII 1054 CCTAGG C^CTAGG BalI 93 TGGCCA TGG^CCA BamHI 29 GGATCC G^GATCC BanII 722 GAGCCC G[GA]GC[CT]^C BbsI 986 GAAGAC GAAGAC(2/6) BclI 158 TGATCA T^GATCA BlnI 1054 CCTAGG C^CTAGG BpuAI 986 GAAGAC GAAGAC(2/6) BsoBI 869 CCCGGG C^[CT]CG[GA]G BspHI 738 TCATGA T^CATGA BsrDI 162 CATTGC GCAATG(2/0) BssSI 325 CACGAG CACGAG(-5/-1) BstAPI 388 GCATGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 342 CCAGGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 93 TGGCCA [CT]^GGCC[GA] EarI 719 GAAGAG CTCTTC(1/4) FbaI 158 TGATCA T^GATCA HgaI 69 GCGTC GACGC(5/10) Ksp632I 719 GAAGAG CTCTTC(1/4) MaeII 583 ACGT A^CGT MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MseI 901 TTAA T^TAA NdeI 251 CATATG CA^TATG NspI 388 GCATGT [GA]CATG^[CT] PflMI 581 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 328 GAGTC GAGTC(4/5) RcaI 738 TCATGA T^CATGA SapI 719 GAAGAGC GCTCTTC(1/4) SexAI 546 ACCTGGT A^CC[AT]GGT SfcI 99 CTACAG C^T[GA][CT]AG SmaI 869 CCCGGG CCC^GGG SmlI 471 CTTGAG C^T[CT][GA]AG StuI 978 AGGCCT AGG^CCT TaqI 125 TCGA T^CGA Tru9I 901 TTAA T^TAA TthHB8I 125 TCGA T^CGA Van91I 581 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 858 TCTAGA T^CTAGA XmaCI 869 CCCGGG C^CCGGG XmaI 869 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 476 GAATTT [GA]^AATT[CT] 683 AAATTT ApoI 476 GAATTT [GA]^AATT[CT] 683 AAATTT AspHI 200 GTGCTC G[AT]GC[AT]^C 1005 GTGCAC BbvI 278 GCTGC GCAGC(8/12) 643 GCTGC BcnI 869 CCCGG CC^[GC]GG 870 CCGGG BsaBI 412 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 492 GATCTGGATC BsiHKAI 200 GTGCTC G[AT]GC[AT]^C 1005 GTGCAC BstEII 456 GGTCACC G^GT[ACGT]ACC 550 GGTTACC BstF5I 1010 CATCC GGATG(2/0) 111 GGATG BstPI 456 GGTCACC G^GT[ACGT]ACC 550 GGTTACC BstYI 29 GGATCC [GA]^GATC[CT] 497 GGATCT Cac8I 397 GCTTGC GC[ACGT]^[ACGT]GC 912 GCTTGC DdeI 363 CTCAG C^T[ACGT]AG 995 CTGAG DsaI 555 CCATGG C^C[GA][CT]GG 791 CCATGG EcoO65I 456 GGTCACC G^GT[ACGT]ACC 550 GGTTACC Fnu4HI 278 GCTGC GC^[ACGT]GC 643 GCTGC FokI 1010 CATCC GGATG(9/13) 111 GGATG HaeIII 94 GGCC GG^CC 979 GGCC HapII 870 CCGG C^CGG 972 CCGG HpaII 870 CCGG C^CGG 972 CCGG ItaI 278 GCTGC GC^[ACGT]GC 643 GCTGC MamI 412 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 492 GATCTGGATC MflI 29 GGATCC [GA]^GATC[CT] 497 GGATCT MslI 97 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 402 CATCAGTGTG MspI 870 CCGG C^CGG 972 CCGG MwoI 388 GCATGTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 637 GCTCCTGCTGC NciI 869 CCCGG CC^[GC]GG 870 CCGGG NcoI 555 CCATGG C^CATGG 791 CCATGG SfaNI 119 GCATC GCATC(5/9) 401 GCATC TseI 278 GCTGC G^C[AT]GC 643 GCTGC XhoII 29 GGATCC [GA]^GATC[CT] 497 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI AlwI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI DpnII EcoRII EcoT14I HinfI HphI MaeI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI StyI TfiI Tsp45I Tsp509I TspRI