(Circular) (Six-base) MAPSORT of: pFN21AE9414.seq Check: 4276 from: 1 to: 5788 pFN21AE9414 5788 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3851 5235 5503 1632 Size: 2219 1384 268 1917 Fragments arranged by size: 2219 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2106 1166 Size: 940 4848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaLI G'TGCA_C Cuts at: 3010 3845 5001 3010 Size: 835 1156 3797 Fragments arranged by size: 3797 1156 835 ApoI r'AATT_y Cuts at: 1493 2481 2688 3097 3323 3475 5584 1493 Size: 988 207 409 226 152 2109 1697 Fragments arranged by size: 2109 1697 988 409 226 207 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2874 3113 1870 Size: 73 931 239 4545 Fragments arranged by size: 4545 931 239 73 AvrII C'CTAG_G Cuts at: 3059 3059 Size: 5788 BamHI G'GATC_C Cuts at: 2034 3118 3485 2034 Size: 1084 367 4337 Fragments arranged by size: 4337 1084 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 4564 618 Size: 325 217 627 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 627 325 217 BanII G_rGCy'C Cuts at: 1391 2731 3107 1391 Size: 1340 376 4072 Fragments arranged by size: 4072 1340 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2998 928 Size: 971 1099 3718 Fragments arranged by size: 3718 1099 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 2163 1548 Size: 615 5173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 3014 3849 5005 1790 Size: 1224 835 1156 2573 Fragments arranged by size: 2573 1224 1156 835 BmgBI CAC'GTC Cuts at: 1318 2589 1318 Size: 1271 4517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4448 1239 Size: 3209 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2497 3913 4778 5076 2497 Size: 1416 865 298 3209 Fragments arranged by size: 3209 1416 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 2421 2501 5782 2421 Size: 80 3281 2427 Fragments arranged by size: 3281 2427 80 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 1178 Size: 30 165 30 1542 30 3991 Fragments arranged by size: 3991 1542 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5788 BseYI C'CCAG_C Cuts at: 2171 2541 4991 5487 2171 Size: 370 2450 496 2472 Fragments arranged by size: 2472 2450 496 370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2209 3014 3849 3934 5005 2209 Size: 805 835 85 1071 2992 Fragments arranged by size: 2992 1071 835 805 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2209 2731 3014 3107 3849 3934 5005 Size: 399 419 522 283 93 742 85 1071 Cuts at: 5005 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 522 419 399 283 93 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 2743 3677 5407 2743 Size: 934 1730 3124 Fragments arranged by size: 3124 1730 934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2166 4283 4457 65 Size: 1183 918 2117 174 1396 Fragments arranged by size: 2117 1396 1183 918 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 2330 3848 4860 2330 Size: 1518 1012 3258 Fragments arranged by size: 3258 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2399 794 Size: 1605 4183 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 2461 2555 1184 Size: 1277 94 4417 Fragments arranged by size: 4417 1277 94 BstXI CCAn_nnnn'nTGG Cuts at: 2354 2354 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2034 2502 3118 3485 3870 3887 5339 5425 Size: 62 468 616 367 385 17 1452 86 Cuts at: 5425 5437 5783 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 616 468 385 367 346 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2560 2796 513 Size: 552 1495 236 3505 Fragments arranged by size: 3505 1495 552 236 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2056 3304 4098 4118 2056 Size: 1248 794 20 3726 Fragments arranged by size: 3726 1248 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3235 4124 Size: 54 1425 50 275 18 268 1137 889 Cuts at: 4124 5753 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1137 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2719 3718 1353 Size: 1366 999 3423 Fragments arranged by size: 3423 1366 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3851 4448 5235 5503 1239 Size: 393 2219 597 787 268 1524 Fragments arranged by size: 2219 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2349 2381 4429 4461 4503 Size: 32 135 32 777 32 2048 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 2048 777 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2254 2915 2988 4902 5086 1305 Size: 949 661 73 1914 184 2007 Fragments arranged by size: 2007 1914 949 661 184 73 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 10 Size: 54 1425 343 268 3698 Fragments arranged by size: 3698 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 2411 3746 4105 4264 518 Size: 648 940 305 1335 359 159 2042 Fragments arranged by size: 2042 1335 940 648 359 305 159 MspA1I CmG'CkG Cuts at: 1606 3204 3881 5029 5274 1606 Size: 1598 677 1148 245 2120 Fragments arranged by size: 2120 1598 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2560 2796 513 Size: 552 1495 236 3505 Fragments arranged by size: 3505 1495 552 236 NdeI CA'TA_TG Cuts at: 387 2257 387 Size: 1870 3918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2397 3146 4691 1132 Size: 97 1168 749 1545 2229 Fragments arranged by size: 2229 1545 1168 749 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2592 1108 Size: 1484 4304 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SapI GCTCTTCn'nnn_ Cuts at: 2719 2719 Size: 5788 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SexAI A'CCwGG_T Cuts at: 2551 2551 Size: 5788 SfcI C'TryA_G Cuts at: 826 1045 2104 3136 4271 4952 5143 826 Size: 219 1059 1032 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 1032 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2876 3115 1872 Size: 1004 239 4545 Fragments arranged by size: 4545 1004 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 2476 3892 4793 5055 820 Size: 197 926 533 1416 901 262 1553 Fragments arranged by size: 1553 1416 926 901 533 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2985 1517 Size: 1468 4320 StyI C'CwwG_G Cuts at: 513 1065 2560 2796 3059 3227 513 Size: 552 1495 236 263 168 3074 Fragments arranged by size: 3074 1495 552 263 236 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2736 3815 3832 3985 4170 4615 325 Size: 1255 1156 1079 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1156 1079 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2803 2835 5748 5780 2803 Size: 32 2913 32 2811 Fragments arranged by size: 2913 2811 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 2863 3124 2863 Size: 261 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2874 3113 1870 Size: 1004 239 4545 Fragments arranged by size: 4545 1004 239 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI