################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MboI MflI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AfaI 727 GTAC GT^AC Alw44I 14 GTGCAC G^TGCAC ApaLI 14 GTGCAC G^TGCAC Asp700I 403 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 14 GTGCAC G[AT]GC[AT]^C AspI 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 372 CCTAGG C^CTAGG BanI 67 GGTGCC G^G[CT][GA]CC BbvI 664 GCTGC GCAGC(8/12) BcnI 399 CCCGG CC^[GC]GG BlnI 372 CCTAGG C^CTAGG BmyI 14 GTGCAC G[AGT]GC[ACT]^C BpmI 511 CTGGAG CTGGAG(16/14) BsaBI 89 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 74 CTCCTC GAGGAG(10/8) BsiHKAI 14 GTGCAC G[AT]GC[AT]^C BsmI 193 GCATTC GAATGC(1/-1) Bsp1286I 14 GTGCAC G[AGT]GC[ACT]^C BstAPI 634 GCAATCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 822 GGTTACC G^GT[ACGT]ACC BstPI 822 GGTTACC G^GT[ACGT]ACC Cac8I 679 GCATGC GC[ACGT]^[ACGT]GC DdeI 446 CTGAG C^T[ACGT]AG DraII 395 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 395 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 822 GGTTACC G^GT[ACGT]ACC EcoT14I 372 CCTAGG C^C[AT][AT]GG EcoT22I 641 ATGCAT ATGCA^T Fnu4HI 664 GCTGC GC^[ACGT]GC HapII 400 CCGG C^CGG HpaII 400 CCGG C^CGG HphI 533 TCACC GGTGA(8/7) ItaI 664 GCTGC GC^[ACGT]GC MaeIII 823 GTTAC ^GT[ACGT]AC MamI 89 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 436 CAATTG C^AATTG MspI 400 CCGG C^CGG MunI 436 CAATTG C^AATTG NciI 399 CCCGG CC^[GC]GG NsiI 641 ATGCAT ATGCA^T NspI 679 GCATGC [GA]CATG^[CT] PflFI 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 693 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 395 GGGACCC [GA]G^G[AT]CC[CT] PstI 79 CTGCAG CTGCA^G RsaI 727 GTAC GT^AC SmlI 42 CTCAAG C^T[CT][GA]AG SphI 679 GCATGC GCATG^C StyI 372 CCTAGG C^C[AT][AT]GG TaqI 135 TCGA T^CGA TseI 664 GCTGC G^C[AT]GC Tth111I 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 135 TCGA T^CGA Van91I 693 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 274 TCTAGA T^CTAGA XcmI 804 CCACAAAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 403 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 786 AGCT AG^CT 933 AGCT AvaII 396 GGACC G^G[AT]CC 690 GGACC BbsI 337 GAAGAC GAAGAC(2/6) 506 GTCTTC BpuAI 337 GAAGAC GAAGAC(2/6) 506 GTCTTC BsmAI 500 GAGAC GTCTC(1/5) 514 GAGAC BsmFI 395 GGGAC GGGAC(10/14) 794 GGGAC Cfr13I 396 GGACC G^G[ACGT]CC 690 GGACC MseI 229 TTAA T^TAA 647 TTAA MslI 348 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 943 CATACTGATG Sau96I 396 GGACC G^G[ACGT]CC 690 GGACC SfaNI 778 GATGC GCATC(5/9) 37 GCATC Tru9I 229 TTAA T^TAA 647 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI ApoI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI EcoRII FokI HincII HindII HinfI MaeI MboII MnlI MvaI MwoI NlaIII NlaIV PleI ScrFI SfcI TfiI Tsp509I TspRI