Restriction map for
pFN21AE9617
(Circular) (Six-base) MAPSORT of: pFN21AE9617.seq Check: 6214 from: 1 to: 5737
pFN21AE9617 5737 bp
With 182 enzymes: SgfI *
March 28, 2012 12:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 2925 3800 5184 5452 1632
Size: 444 849 875 1384 268 1917
Fragments arranged by size:
1917 1384 875 849 444 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2101 2133 2101
Size: 32 5705
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaLI G'TGCA_C
Cuts at: 2019 3794 4950 2019
Size: 1775 1156 2806
Fragments arranged by size:
2806 1775 1156
ApoI r'AATT_y
Cuts at: 1493 2148 2249 2717 2892 3046 3272 3424 5533
Size: 655 101 468 175 154 226 152 2109
Cuts at: 5533 1493
Size: 1697
Fragments arranged by size:
2109 1697 655 468 226 175 154 152
101
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 1870
Size: 73 1119 4545
Fragments arranged by size:
4545 1119 73
AvrII C'CTAG_G
Cuts at: 2377 2377
Size: 5737
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 3058 4513 618
Size: 325 217 627 285 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 3056 1391
Size: 1665 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2349 2504 928
Size: 971 450 155 4161
Fragments arranged by size:
4161 971 450 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BfrBI ATG'CAT
Cuts at: 2648 2648
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2023 3798 4954 1790
Size: 233 1775 1156 2573
Fragments arranged by size:
2573 1775 1156 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3468 4426
Size: 416 53 83 399 24 732 1319 958
Cuts at: 4426 442
Size: 1753
Fragments arranged by size:
1753 1319 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 4397 1239
Size: 1298 1860 2579
Fragments arranged by size:
2579 1860 1298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2032 3862 4727 5025 2032
Size: 1830 865 298 2744
Fragments arranged by size:
2744 1830 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 2098 5731 2098
Size: 3633 2104
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2070 1186
Size: 884 4853
BseYI C'CCAG_C
Cuts at: 4940 5436 4940
Size: 496 5241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 3798 3883 4954 2023
Size: 1775 85 1071 2806
Fragments arranged by size:
2806 1775 1071 85
BsmI GAATG_Cn'
Cuts at: 2198 3248 3341 2198
Size: 1050 93 4594
Fragments arranged by size:
4594 1050 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 3056 3798 3883 4954 1391
Size: 399 233 1033 742 85 1071 2174
Fragments arranged by size:
2174 1071 1033 742 399 233 85
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3092 844
Size: 2248 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2645 794
Size: 1851 3886
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 4094
BstYI r'GATC_y
Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732
Size: 1095 367 385 17 1452 86 12 346
Cuts at: 5732 1972
Size: 1977
Fragments arranged by size:
1977 1452 1095 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3184 4073 5702
Size: 54 1425 50 275 18 1354 889 1629
Cuts at: 5702 8
Size: 43
Fragments arranged by size:
1629 1425 1354 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3667 1353
Size: 2314 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2537 2925 3800 4397 5184 5452
Size: 393 444 461 388 875 597 787 268
Cuts at: 5452 1239
Size: 1524
Fragments arranged by size:
1524 875 787 597 461 444 393 388
268
EcoO109I rG'GnC_Cy
Cuts at: 2401 2401
Size: 5737
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2432 2432
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2959 2991 4378
Size: 32 135 32 550 32 805 32 1387
Cuts at: 4378 4410 4452 4484 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1387 805 550 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 2182 2845 3081 3327 669
Size: 988 281 93 151 663 236 246 3079
Fragments arranged by size:
3079 988 663 281 246 236 151 93
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 2441 3336 2441
Size: 895 4842
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4851 5035 1305
Size: 3546 184 2007
Fragments arranged by size:
3546 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2952 3695 4054 4213 518
Size: 648 1191 595 743 359 159 2042
Fragments arranged by size:
2042 1191 743 648 595 359 159
MspA1I CmG'CkG
Cuts at: 1606 3153 3830 4978 5223 1606
Size: 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NsiI A_TGCA'T
Cuts at: 2650 2650
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 2688 3095 4640 1132
Size: 97 1459 407 1545 2229
Fragments arranged by size:
2229 1545 1459 407 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4141
PfoI T'CCnGG_A
Cuts at: 2660 2660
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 2401
Size: 5737
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 2088 3089 830
Size: 1258 1001 3478
Fragments arranged by size:
3478 1258 1001
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SanDI GG'GwC_CC
Cuts at: 2401 2401
Size: 5737
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2084 2257 2737 2934 3085 4220 4901
Size: 219 1039 173 480 197 151 1135 681
Cuts at: 4901 5092 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1039 681 480 219 197 191
173 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 3841 4742 5004 820
Size: 197 926 104 1794 901 262 1553
Fragments arranged by size:
1794 1553 926 901 262 197 104
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 2688 3095 1132
Size: 1556 407 3774
Fragments arranged by size:
3774 1556 407
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 2377 3176 513
Size: 552 939 373 799 3074
Fragments arranged by size:
3074 939 799 552 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3764 3781 3934 4119 4564 325
Size: 1255 512 1672 17 153 185 445 1498
Fragments arranged by size:
1672 1498 1255 512 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 5697 5729 2313
Size: 32 3352 32 2321
Fragments arranged by size:
3352 2321 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2804 1316
Size: 1488 4249
XbaI T'CTAG_A
Cuts at: 2279 3073 2279
Size: 794 4943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2816 2816
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 2412 3866 2412
Size: 1454 4283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI