################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BmyI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 216 GTATAC GT^[AC][GT]AC AciI 780 GCGG CCGC(-3/-1) AcsI 31 GAATTT [GA]^AATT[CT] AlwNI 69 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTT [GA]^AATT[CT] AseI 604 ATTAAT AT^TAAT AsnI 604 ATTAAT AT^TAAT BalI 371 TGGCCA TGG^CCA BbsI 872 GTCTTC GAAGAC(2/6) BfaI 935 CTAG C^TAG BglI 424 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 552 GCTGAGC GC^T[ACGT]AGC Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC BpuAI 872 GTCTTC GAAGAC(2/6) BsgI 163 CTGCAC GTGCAG(16/14) BsmFI 464 GTCCC GGGAC(10/14) BsmI 331 GAATGC GAATGC(1/-1) BspHI 641 TCATGA T^CATGA Bst1107I 216 GTATAC GTA^TAC BstZ17I 216 GTATAC GTA^TAC CelII 552 GCTGAGC GC^T[ACGT]AGC DraIII 416 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 350 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EarI 789 GAAGAG CTCTTC(1/4) HgaI 325 GCGTC GACGC(5/10) HphI 110 TCACC GGTGA(8/7) Ksp632I 789 GAAGAG CTCTTC(1/4) MaeI 935 CTAG C^TAG MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA NcoI 350 CCATGG C^CATGG NlaIV 383 GGCCCC GG[ACGT]^[ACGT]CC NspI 125 ACATGC [GA]CATG^[CT] PflMI 312 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 604 ATTAAT AT^TAAT RcaI 641 TCATGA T^CATGA SapI 789 GAAGAGC GCTCTTC(1/4) SspI 250 AATATT AAT^ATT StuI 707 AGGCCT AGG^CCT Tsp45I 536 GTGAC ^GT[GC]AC Van91I 312 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 177 GTAC GT^AC 838 GTAC AlwI 3 GGATC GGATC(4/5) 659 GGATC AvaII 741 GGTCC G^G[AT]CC 908 GGACC BsiYI 312 CCACTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 374 CCATTTGTCGG BslI 312 CCACTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 374 CCATTTGTCGG BsrI 150 ACTGG ACTGG(1/-1) 53 CCAGT BssKI 24 CCTGG ^CC[ACGT]GG 369 CCTGG BstNI 24 CCTGG CC^[AT]GG 369 CCTGG Cac8I 74 GCTGGC GC[ACGT]^[ACGT]GC 562 GCCTGC DpnII 4 GATC ^GATC 660 GATC EcoRII 24 CCTGG ^CC[AT]GG 369 CCTGG MboI 4 GATC ^GATC 660 GATC MseI 605 TTAA T^TAA 893 TTAA MvaI 24 CCTGG CC^[AT]GG 369 CCTGG NdeII 4 GATC ^GATC 660 GATC RsaI 177 GTAC GT^AC 838 GTAC Sau3AI 4 GATC ^GATC 660 GATC ScrFI 24 CCTGG CC^[ACGT]GG 369 CCTGG SfcI 824 CTATAG C^T[GA][CT]AG 867 CTACAG Tru9I 605 TTAA T^TAA 893 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BbvI BsaJI BstF5I Cfr13I DdeI EcoT14I Fnu4HI FokI HaeIII ItaI MaeIII MboII MnlI MslI MwoI NlaIII Sau96I SfaNI StyI TseI Tsp509I TspRI