################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MluI MseI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 737 TCCGGA T^CCGGA AviII 141 TGCGCA TGC^GCA BalI 437 TGGCCA TGG^CCA BamHI 523 GGATCC G^GATCC BciVI 506 GTATCC GTATCC(6/5) BfaI 664 CTAG C^TAG BglII 293 AGATCT A^GATCT BlpI 636 GCTGAGC GC^T[ACGT]AGC BpmI 289 CTGGAG CTGGAG(16/14) Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC BsaJI 362 CCGTGG C^C[ACGT][ACGT]GG BsaWI 737 TCCGGA [AT]^CCGG[AT] BseAI 737 TCCGGA T^CCGGA BsmAI 44 GTCTC GTCTC(1/5) BsmFI 890 GGGAC GGGAC(10/14) BsmI 761 GAATGC GAATGC(1/-1) BspEI 737 TCCGGA T^CCGGA BspHI 488 TCATGA T^CATGA BstXI 396 CCACTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 636 GCTGAGC GC^T[ACGT]AGC CfoI 142 GCGC GCG^C Cfr13I 243 GGCCC G^G[ACGT]CC DsaI 362 CCGTGG C^C[GA][CT]GG FauI 135 CCCGC CCCGC(4/6) FspI 141 TGCGCA TGC^GCA HapII 738 CCGG C^CGG HhaI 142 GCGC GCG^C HinP1I 142 GCGC G^CGC HincII 81 GTTGAC GT[CT]^[GA]AC HindII 81 GTTGAC GT[CT]^[GA]AC HpaII 738 CCGG C^CGG MaeI 664 CTAG C^TAG MfeI 899 CAATTG C^AATTG MluNI 437 TGGCCA TGG^CCA MroI 737 TCCGGA T^CCGGA MscI 437 TGGCCA TGG^CCA MspI 738 CCGG C^CGG MunI 899 CAATTG C^AATTG NspI 138 GCATGC [GA]CATG^[CT] PleI 42 GAGTC GAGTC(4/5) PstI 379 CTGCAG CTGCA^G RcaI 488 TCATGA T^CATGA SapI 351 GAAGAGC GCTCTTC(1/4) Sau96I 243 GGCCC G^G[ACGT]CC SphI 138 GCATGC GCATG^C SspI 8 AATATT AAT^ATT StuI 788 AGGCCT AGG^CCT XcmI 510 CCAGCTGACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AciI 136 CCGC CCGC(-3/-1) 694 GCGG AfaI 387 GTAC GT^AC 450 GTAC BsgI 247 CTGCAC GTGCAG(16/14) 648 CTGCAC BsrDI 720 CATTGC GCAATG(2/0) 433 GCAATG BsrI 202 ACTGG ACTGG(1/-1) 89 CCAGT EaeI 86 CGGCCA [CT]^GGCC[GA] 437 TGGCCA HphI 108 GGTGA GGTGA(8/7) 810 TCACC NlaIV 523 GGATCC GG[ACGT]^[ACGT]CC 592 GGCTCC PvuII 511 CAGCTG CAG^CTG 770 CAGCTG RsaI 387 GTAC GT^AC 450 GTAC SfaNI 324 GATGC GCATC(5/9) 679 GATGC SfcI 379 CTGCAG C^T[GA][CT]AG 948 CTATAG Tsp45I 109 GTGAC ^GT[GC]AC 907 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI AlwI BbvI BsiYI BslI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MslI MspA1I MvaI MwoI NdeII NlaIII Sau3AI ScrFI TfiI TseI Tsp509I TspRI XhoII