(Circular) (Six-base) MAPSORT of: pFN21AE5490.seq Check: 8908 from: 1 to: 5734 pFN21AE5490 5734 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 3797 5181 5449 1632 Size: 1167 998 1384 268 1917 Fragments arranged by size: 1917 1384 1167 998 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 2154 2636 1166 Size: 988 482 4264 Fragments arranged by size: 4264 988 482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2267 1184 Size: 1083 4651 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 3059 1870 Size: 73 1116 4545 Fragments arranged by size: 4545 1116 73 BamHI G'GATC_C Cuts at: 2528 3064 3431 2528 Size: 536 367 4831 Fragments arranged by size: 4831 536 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 4510 618 Size: 325 217 627 1268 1455 1842 Fragments arranged by size: 1842 1455 1268 627 325 217 BanII G_rGCy'C Cuts at: 1391 3053 1391 Size: 1662 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2522 3626 4836 2522 Size: 1104 1210 3420 Fragments arranged by size: 3420 1210 1104 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2298 5729 2298 Size: 3431 2303 BlpI GC'TnA_GC Cuts at: 2642 3151 2642 Size: 509 5225 Bme1580I G_kGCm'C Cuts at: 1790 3795 4951 1790 Size: 2005 1156 2573 Fragments arranged by size: 2573 2005 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2177 2177 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 4394 1239 Size: 1076 2079 2579 Fragments arranged by size: 2579 2079 1076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2742 4167 4565 4839 4986 5397 2742 Size: 1425 398 274 147 411 3079 Fragments arranged by size: 3079 1425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5509 Fragments arranged by size: 5509 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5734 BseYI C'CCAG_C Cuts at: 3026 4937 5433 3026 Size: 1911 496 3327 Fragments arranged by size: 3327 1911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2638 1163 Size: 573 501 401 4259 Fragments arranged by size: 4259 573 501 401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 2772 3245 3338 2772 Size: 473 93 5168 Fragments arranged by size: 5168 473 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3053 3795 3880 4951 1391 Size: 399 1263 742 85 1071 2174 Fragments arranged by size: 2174 1263 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2742 5397 2742 Size: 2655 3079 BspHI T'CATG_A Cuts at: 2493 3623 5353 2493 Size: 1130 1730 2874 Fragments arranged by size: 2874 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3089 844 Size: 2245 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2445 2724 4229 4403 65 Size: 1183 1197 279 1505 174 1396 Fragments arranged by size: 1505 1396 1197 1183 279 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2408 2408 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2298 2528 2607 3064 3431 3816 3833 5285 Size: 326 230 79 457 367 385 17 1452 Cuts at: 5285 5371 5383 5729 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 457 385 367 346 326 230 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2367 513 Size: 552 1302 3880 Fragments arranged by size: 3880 1302 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3250 4044 4064 2851 Size: 399 794 20 4521 Fragments arranged by size: 4521 794 399 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2442 3181 Size: 54 1425 50 275 18 261 351 739 Cuts at: 3181 4070 5699 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 739 351 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2351 3005 3664 1353 Size: 779 219 654 659 3423 Fragments arranged by size: 3423 779 659 654 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2799 3797 4394 5181 5449 1239 Size: 393 683 484 998 597 787 268 1524 Fragments arranged by size: 1524 998 787 683 597 484 393 268 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 2148 4240 2148 Size: 2092 3642 FspAI rTGC'GCAy Cuts at: 2148 2148 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373 Size: 32 135 32 2803 32 42 32 2626 Fragments arranged by size: 2803 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2088 3078 3324 669 Size: 988 281 150 990 246 3079 Fragments arranged by size: 3079 990 988 281 246 150 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 2904 3333 2904 Size: 429 5305 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2506 2625 4848 5032 1305 Size: 1201 119 2223 184 2007 Fragments arranged by size: 2223 2007 1201 184 119 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 10 Size: 54 1425 343 612 3300 Fragments arranged by size: 3300 1425 612 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 2237 2366 2636 3692 4051 4210 Size: 648 988 83 129 270 1056 359 159 Cuts at: 4210 518 Size: 2042 Fragments arranged by size: 2042 1056 988 648 359 270 159 129 83 MspA1I CmG'CkG Cuts at: 1606 2518 2699 2777 3150 3827 4975 5220 1606 Size: 912 181 78 373 677 1148 245 2120 Fragments arranged by size: 2120 1148 912 677 373 245 181 78 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2147 3092 4637 1132 Size: 97 918 945 1545 2229 Fragments arranged by size: 2229 1545 945 918 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PfoI T'CCnGG_A Cuts at: 2292 2292 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PstI C_TGCA'G Cuts at: 830 2388 3086 830 Size: 1558 698 3478 Fragments arranged by size: 3478 1558 698 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2518 2777 2518 Size: 259 5475 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SapI GCTCTTCn'nnn_ Cuts at: 2351 2351 Size: 5734 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2384 2953 3082 4217 4898 5089 826 Size: 219 1339 569 129 1135 681 191 1471 Fragments arranged by size: 1471 1339 1135 681 569 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 2147 3092 1132 Size: 1015 945 3774 Fragments arranged by size: 3774 1015 945 SspI AAT'ATT Cuts at: 5 52 2015 3658 5 Size: 47 1963 1643 2081 Fragments arranged by size: 2081 1963 1643 47 StuI AGG'CCT Cuts at: 1517 2795 1517 Size: 1278 4456 StyI C'CwwG_G Cuts at: 513 1065 3173 513 Size: 552 2108 3074 Fragments arranged by size: 3074 2108 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3980 5692 Size: 274 80 33 51 1443 414 1589 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1589 1443 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2522 2522 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI