################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfaI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AciI 8 GCGG CCGC(-3/-1) AcsI 31 GAATTT [GA]^AATT[CT] AflIII 572 ACACGT A^C[GA][CT]GT AluI 56 AGCT AG^CT AlwI 335 GGATC GGATC(4/5) AlwNI 23 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 508 GGGCCC GGGCC^C ApoI 31 GAATTT [GA]^AATT[CT] AseI 129 ATTAAT AT^TAAT AsnI 129 ATTAAT AT^TAAT AspHI 422 GTGCTC G[AT]GC[AT]^C AspI 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 472 CCCGAG C^[CT]CG[GA]G AvaII 543 GGTCC G^G[AT]CC BalI 353 TGGCCA TGG^CCA BanII 508 GGGCCC G[GA]GC[CT]^C BbvCI 52 CCTCAGC CCTCAGC(-5/-2) BbvI 287 GCTGC GCAGC(8/12) BclI 401 TGATCA T^GATCA BcnI 413 CCCGG CC^[GC]GG BlpI 552 GCTAAGC GC^T[ACGT]AGC Bpu1102I 552 GCTAAGC GC^T[ACGT]AGC BsgI 719 GTGCAG GTGCAG(16/14) BsiHKAI 422 GTGCTC G[AT]GC[AT]^C BsmAI 71 GTCTC GTCTC(1/5) BsmFI 544 GTCCC GGGAC(10/14) BsoBI 472 CCCGAG C^[CT]CG[GA]G Bsp1407I 565 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BspMI 436 GCAGGT ACCTGC(4/8) BsrBI 6 GAGCGG CCGCTC(-3/-3) BsrGI 565 TGTACA T^GTACA Cac8I 721 GCAGGC GC[ACGT]^[ACGT]GC CelII 552 GCTAAGC GC^T[ACGT]AGC DsaI 350 CCATGG C^C[GA][CT]GG EaeI 353 TGGCCA [CT]^GGCC[GA] EcoT14I 350 CCATGG C^C[AT][AT]GG FbaI 401 TGATCA T^GATCA Fnu4HI 287 GCTGC GC^[ACGT]GC HapII 414 CCGG C^CGG HpaII 414 CCGG C^CGG ItaI 287 GCTGC GC^[ACGT]GC MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MspI 414 CCGG C^CGG NciI 413 CCCGG CC^[GC]GG NcoI 350 CCATGG C^CATGG PflFI 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 129 ATTAAT AT^TAAT PstI 434 CTGCAG CTGCA^G RcaI 404 TCATGA T^CATGA SfcI 434 CTGCAG C^T[GA][CT]AG SspBI 565 TGTACA T^GTACA StyI 350 CCATGG C^C[AT][AT]GG TseI 287 GCTGC G^C[AT]GC Tth111I 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbsI 736 GTCTTC GAAGAC(2/6) 914 GTCTTC BglII 209 AGATCT A^GATCT 527 AGATCT BmyI 422 GTGCTC G[AGT]GC[ACT]^C 508 GGGCCC BpmI 205 CTGGAG CTGGAG(16/14) 938 CTGGAG BpuAI 736 GTCTTC GAAGAC(2/6) 914 GTCTTC BsaJI 350 CCATGG C^C[ACGT][ACGT]GG 412 CCCCGG BsiYI 499 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 726 CCACCTCATGG BslI 499 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 726 CCACCTCATGG Bsp1286I 422 GTGCTC G[AGT]GC[ACT]^C 508 GGGCCC BsrDI 636 CATTGC GCAATG(2/0) 816 CATTGC BstF5I 63 GGATG GGATG(2/0) 594 GGATG BstNI 558 CCTGG CC^[AT]GG 937 CCTGG EcoRII 558 CCTGG ^CC[AT]GG 937 CCTGG FokI 63 GGATG GGATG(9/13) 594 GGATG MaeII 368 ACGT A^CGT 574 ACGT MseI 130 TTAA T^TAA 899 TTAA MslI 273 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 573 CACGTCCATG MvaI 558 CCTGG CC^[AT]GG 937 CCTGG SfaNI 240 GATGC GCATC(5/9) 290 GCATC Tru9I 130 TTAA T^TAA 899 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI BsrI BssKI BstYI Cfr13I DdeI DpnII EarI HaeIII HinfI Ksp632I MaeIII MboI MboII MflI MnlI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI Tsp45I Tsp509I TspRI XhoII