################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BstAPI BstEII BstPI BstUI CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AflIII 721 ACGTGT A^C[GA][CT]GT AlwI 482 GGATC GGATC(4/5) AocI 876 CCTGAGG CC^T[ACGT]AGG AspHI 191 GTGCTC G[AT]GC[AT]^C BalI 368 TGGCCA TGG^CCA BanII 6 GAGCCC G[GA]GC[CT]^C BbrPI 720 CACGTG CAC^GTG BfaI 248 CTAG C^TAG BsaBI 636 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 264 GAGGAG GAGGAG(10/8) BsgI 561 CTGCAC GTGCAG(16/14) BsiHKAI 191 GTGCTC G[AT]GC[AT]^C BsmFI 952 GGGAC GGGAC(10/14) BsrDI 822 CATTGC GCAATG(2/0) BsrFI 316 ACCGGC [GA]^CCGG[CT] BssSI 572 CACGAG CACGAG(-5/-1) BstBI 507 TTCGAA TT^CGAA BstF5I 600 GGATG GGATG(2/0) BstXI 821 CCATTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 482 GGATCT [GA]^GATC[CT] Bsu36I 876 CCTGAGG CC^T[ACGT]AGG Cfr10I 316 ACCGGC [GA]^CCGG[CT] DpnII 483 GATC ^GATC DraII 952 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 368 TGGCCA [CT]^GGCC[GA] Eco81I 876 CCTGAGG CC^T[ACGT]AGG EcoO109I 952 GGGACCT [GA]G^G[ACGT]CC[CT] FokI 600 GGATG GGATG(9/13) HapII 317 CCGG C^CGG HgaI 552 GCGTC GACGC(5/10) HpaII 317 CCGG C^CGG MaeI 248 CTAG C^TAG MamI 636 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 483 GATC ^GATC MflI 482 GGATCT [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MspI 317 CCGG C^CGG NdeI 372 CATATG CA^TATG NdeII 483 GATC ^GATC NlaIV 952 GGGACC GG[ACGT]^[ACGT]CC NspV 507 TTCGAA TT^CGAA PleI 768 GACTC GAGTC(4/5) PmaCI 720 CACGTG CAC^GTG PmlI 720 CACGTG CAC^GTG PpuMI 952 GGGACCT [GA]G^G[AT]CC[CT] PstI 292 CTGCAG CTGCA^G Sau3AI 483 GATC ^GATC SexAI 125 ACCTGGT A^CC[AT]GGT SfuI 507 TTCGAA TT^CGAA SmlI 957 CTCAAG C^T[CT][GA]AG SnaBI 680 TACGTA TAC^GTA Tsp509I 675 AATT ^AATT XhoII 482 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 177 GTATAC GT^[AC][GT]AC 216 GTATAC AfaI 419 GTAC GT^AC 777 GTAC AvaII 144 GGTCC G^G[AT]CC 953 GGACC BbvI 377 GCAGC GCAGC(8/12) 549 GCTGC BmyI 6 GAGCCC G[AGT]GC[ACT]^C 191 GTGCTC BpmI 205 CTGGAG CTGGAG(16/14) 695 CTGGAG BsaAI 680 TACGTA [CT]AC^GT[GA] 720 CACGTG BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG 949 CCAGGG BsiYI 360 CCACTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 496 CCATTTTGTGG BslI 360 CCACTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 496 CCATTTTGTGG Bsp1286I 6 GAGCCC G[AGT]GC[ACT]^C 191 GTGCTC BspHI 401 TCATGA T^CATGA 584 TCATGA BsrI 147 CCAGT ACTGG(1/-1) 544 CCAGT Bst1107I 177 GTATAC GTA^TAC 216 GTATAC BstZ17I 177 GTATAC GTA^TAC 216 GTATAC Cac8I 251 GCCTGC GC[ACGT]^[ACGT]GC 559 GCCTGC Cfr13I 144 GGTCC G^G[ACGT]CC 953 GGACC HphI 159 TCACC GGTGA(8/7) 513 TCACC MaeII 681 ACGT A^CGT 721 ACGT MaeIII 533 GTGAC ^GT[ACGT]AC 766 GTGAC MseI 65 TTAA T^TAA 433 TTAA MspA1I 629 CAGCTG C[AC]G^C[GT]G 692 CAGCTG PflMI 360 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCATTTTGTGG PvuII 629 CAGCTG CAG^CTG 692 CAGCTG RcaI 401 TCATGA T^CATGA 584 TCATGA RsaI 419 GTAC GT^AC 777 GTAC SapI 93 GCTCTTC GCTCTTC(1/4) 750 GCTCTTC Sau96I 144 GGTCC G^G[ACGT]CC 953 GGACC SfcI 292 CTGCAG C^T[GA][CT]AG 870 CTATAG TaqI 15 TCGA T^CGA 508 TCGA TfiI 510 GAATC G^A[AT]TC 588 GATTC Tru9I 65 TTAA T^TAA 433 TTAA TseI 377 GCAGC G^C[AT]GC 549 GCTGC Tsp45I 533 GTGAC ^GT[GC]AC 766 GTGAC TthHB8I 15 TCGA T^CGA 508 TCGA Van91I 360 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCATTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AluI BsmAI BssKI BstNI DdeI EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MboII MnlI MslI MvaI NlaIII ScrFI SfaNI TspRI