(Circular) (Six-base) MAPSORT of: pFN21AE6086.seq Check: 6994 from: 1 to: 5677 pFN21AE6086 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2183 2222 3020 1937 Size: 246 39 798 4594 Fragments arranged by size: 4594 798 246 39 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2726 4576 5553 1203 Size: 1523 1850 977 1327 Fragments arranged by size: 1850 1523 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 3420 4992 3420 Size: 1572 4105 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 3002 1870 Size: 73 1059 4545 Fragments arranged by size: 4545 1059 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 4453 618 Size: 325 217 627 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2996 1391 Size: 624 981 4072 Fragments arranged by size: 4072 981 624 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 3408 4366 Size: 416 53 83 399 24 1126 865 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1126 958 865 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2721 4337 1239 Size: 992 490 1616 2579 Fragments arranged by size: 2579 1616 992 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2947 3802 4667 4965 2947 Size: 855 865 298 3659 Fragments arranged by size: 3659 865 855 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2687 2727 3468 493 Size: 711 1483 40 741 2702 Fragments arranged by size: 2702 1483 741 711 40 BsaBI GATnn'nnATC Cuts at: 2645 5671 2645 Size: 3026 2651 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 4621 Fragments arranged by size: 4621 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 1186 Size: 1098 4579 BseYI C'CCAG_C Cuts at: 4880 5376 4880 Size: 496 5181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2551 1163 Size: 573 815 4289 Fragments arranged by size: 4289 815 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2200 3738 3823 4894 2200 Size: 1538 85 1071 2983 Fragments arranged by size: 2983 1538 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2200 2996 3738 3823 4894 1391 Size: 399 225 185 796 742 85 1071 2174 Fragments arranged by size: 2174 1071 796 742 399 225 185 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 2406 2589 3566 5296 2406 Size: 183 977 1730 2787 Fragments arranged by size: 2787 1730 977 183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4172 4346 65 Size: 1183 1578 1346 174 1396 Fragments arranged by size: 1578 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 4321 4508 1700 Size: 110 511 2000 187 2869 Fragments arranged by size: 2869 2000 511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 2577 3737 4749 2577 Size: 1160 1012 3505 Fragments arranged by size: 3505 1160 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 2513 4481 2513 Size: 1968 3709 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2487 3007 3374 3759 3776 5228 5314 5326 Size: 515 520 367 385 17 1452 86 12 Cuts at: 5326 5672 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 520 515 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2184 2223 2184 Size: 39 5638 Bsu36I CC'TnA_GG Cuts at: 2882 2882 Size: 5677 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4186 Fragments arranged by size: 4186 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2093 2495 2625 2773 3193 3987 4007 2093 Size: 402 130 148 420 794 20 3763 Fragments arranged by size: 3763 794 420 402 148 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 3124 4013 Size: 54 1425 50 275 18 543 751 889 Cuts at: 4013 5642 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 751 543 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 2394 2762 3607 1353 Size: 752 289 368 845 3423 Fragments arranged by size: 3423 845 752 368 289 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2721 3740 4337 5124 5392 1239 Size: 393 599 490 1019 597 787 268 1524 Fragments arranged by size: 1524 1019 787 599 597 490 393 268 EcoO109I rG'GnC_Cy Cuts at: 2958 2958 Size: 5677 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2571 2603 4318 Size: 32 135 32 430 32 537 32 1715 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1715 537 430 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 4791 4975 1305 Size: 799 2687 184 2007 Fragments arranged by size: 2687 2007 799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3312 Fragments arranged by size: 3312 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2177 2588 2651 3635 3994 4153 Size: 648 978 33 411 63 984 359 159 Cuts at: 4153 518 Size: 2042 Fragments arranged by size: 2042 984 978 648 411 359 159 63 33 MspA1I CmG'CkG Cuts at: 1606 2636 2699 3093 3770 4918 5163 1606 Size: 1030 63 394 677 1148 245 2120 Fragments arranged by size: 2120 1148 1030 677 394 245 63 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4186 Fragments arranged by size: 4186 939 552 NdeI CA'TA_TG Cuts at: 387 2378 387 Size: 1991 3686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 2507 1108 Size: 1263 136 4278 Fragments arranged by size: 4278 1263 136 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PmlI CAC'GTG Cuts at: 2727 2727 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2958 2958 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PstI C_TGCA'G Cuts at: 830 2301 3029 830 Size: 1471 728 3478 Fragments arranged by size: 3478 1471 728 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2636 2699 2636 Size: 63 5614 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SapI GCTCTTCn'nnn_ Cuts at: 2105 2762 2105 Size: 657 5020 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SexAI A'CCwGG_T Cuts at: 2130 2130 Size: 5677 SfcI C'TryA_G Cuts at: 826 1045 2297 2875 3025 4160 4841 5032 826 Size: 219 1252 578 150 1135 681 191 1471 Fragments arranged by size: 1471 1252 1135 681 578 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2962 3781 4682 4944 820 Size: 197 926 1019 819 901 262 1553 Fragments arranged by size: 1553 1019 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 2687 3468 493 Size: 2194 781 2702 Fragments arranged by size: 2702 2194 781 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2004 3116 513 Size: 552 939 1112 3074 Fragments arranged by size: 3074 1112 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2455 2487 5637 5669 2455 Size: 32 3150 32 2463 Fragments arranged by size: 3150 2463 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI