################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BanII BbeI BbrPI BbvI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FauI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KspI MaeI MaeII MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I TseI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 673 GGTACC G^GTACC AciI 411 CCGC CCGC(-3/-1) AflIII 111 ACATGT A^C[GA][CT]GT Alw44I 239 GTGCAC G^TGCAC ApaLI 239 GTGCAC G^TGCAC Asp718I 673 GGTACC G^GTACC AspHI 239 GTGCAC G[AT]GC[AT]^C AvaII 206 GGTCC G^G[AT]CC BamHI 728 GGATCC G^GATCC BanI 673 GGTACC G^G[CT][GA]CC BclI 176 TGATCA T^GATCA BglII 297 AGATCT A^GATCT BmyI 239 GTGCAC G[AGT]GC[ACT]^C BsaBI 51 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 256 GAGACC GGTCTC(1/5) BsgI 527 CTGCAC GTGCAG(16/14) BsiHKAI 239 GTGCAC G[AT]GC[AT]^C Bsp1286I 239 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 114 TGTACA T^GTACA BspLU11I 111 ACATGT A^CATGT BsrGI 114 TGTACA T^GTACA BsrI 303 ACTGG ACTGG(1/-1) BssKI 417 CCTGG ^CC[ACGT]GG BstF5I 690 CATCC GGATG(2/0) BstNI 417 CCTGG CC^[AT]GG BstXI 284 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 206 GGTCC G^G[ACGT]CC DraII 205 AGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 785 CCGTGG C^C[GA][CT]GG EarI 229 CTCTTC CTCTTC(1/4) EcoO109I 205 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 417 CCTGG ^CC[AT]GG EcoRV 440 GATATC GAT^ATC EcoT14I 308 CCAAGG C^C[AT][AT]GG FbaI 176 TGATCA T^GATCA FokI 690 CATCC GGATG(9/13) HinfI 544 GACTC G^A[ACGT]TC KpnI 673 GGTACC GGTAC^C Ksp632I 229 CTCTTC CTCTTC(1/4) MamI 51 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 737 CAGCTG C[AC]G^C[GT]G MvaI 417 CCTGG CC^[AT]GG NdeI 586 CATATG CA^TATG NspI 111 ACATGT [GA]CATG^[CT] PflMI 585 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 544 GACTC GAGTC(4/5) PpuMI 205 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 737 CAGCTG CAG^CTG Sau96I 206 GGTCC G^G[ACGT]CC ScrFI 417 CCTGG CC^[ACGT]GG SspBI 114 TGTACA T^GTACA StuI 755 AGGCCT AGG^CCT StyI 308 CCAAGG C^C[AT][AT]GG Tsp45I 328 GTCAC ^GT[GC]AC Van91I 585 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 340 AGCT AG^CT 738 AGCT AlwI 729 GATCC GGATC(4/5) 728 GGATC AlwNI 521 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 656 CAGTGTCTG BalI 305 TGGCCA TGG^CCA 419 TGGCCA BbsI 136 GTCTTC GAAGAC(2/6) 703 GTCTTC BbvCI 891 CCTCAGC CCTCAGC(-5/-2) 739 GCTGAGG BpuAI 136 GTCTTC GAAGAC(2/6) 703 GTCTTC BsaJI 308 CCAAGG C^C[ACGT][ACGT]GG 785 CCGTGG BspMI 259 ACCTGC ACCTGC(4/8) 166 GCAGGT BstYI 297 AGATCT [GA]^GATC[CT] 728 GGATCC EaeI 305 TGGCCA [CT]^GGCC[GA] 419 TGGCCA EcoRI 79 GAATTC G^AATTC 634 GAATTC MflI 297 AGATCT [GA]^GATC[CT] 728 GGATCC MluNI 305 TGGCCA TGG^CCA 419 TGGCCA MscI 305 TGGCCA TGG^CCA 419 TGGCCA MslI 216 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 393 CACTTTGATG MwoI 490 GCTTGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 494 GCACTGCAGGC PshAI 273 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 696 GACTGATGTC PstI 497 CTGCAG CTGCA^G 552 CTGCAG SfaNI 288 GATGC GCATC(5/9) 811 GCATC SfcI 497 CTGCAG C^T[GA][CT]AG 552 CTGCAG XhoII 297 AGATCT [GA]^GATC[CT] 728 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AfaI AocI ApoI Asp700I BsiYI BslI BsmAI BsmFI Bsu36I Cac8I DdeI DpnII Eco81I HaeIII HphI MaeIII MboI MboII MnlI NdeII NlaIII NlaIV RsaI Sau3AI Tsp509I TspRI XmnI