(Circular) (Six-base) MAPSORT of: pFN21AE6096.seq Check: 2814 from: 1 to: 5683 pFN21AE6096 5683 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 2678 3004 2678 Size: 326 5357 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2724 3746 5130 5398 1632 Size: 467 625 1022 1384 268 1917 Fragments arranged by size: 1917 1384 1022 625 467 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2116 4582 5559 1203 Size: 913 2466 977 1327 Fragments arranged by size: 2466 1327 977 913 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2531 2666 3426 4998 2531 Size: 135 760 1572 3216 Fragments arranged by size: 3216 1572 760 135 ApaLI G'TGCA_C Cuts at: 2244 3740 4896 2244 Size: 1496 1156 3031 Fragments arranged by size: 3031 1496 1156 ApoI r'AATT_y Cuts at: 1493 2037 2084 2639 2992 3218 3370 5479 1493 Size: 544 47 555 353 226 152 2109 1697 Fragments arranged by size: 2109 1697 555 544 353 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 3008 1870 Size: 73 1065 4545 Fragments arranged by size: 4545 1065 73 BamHI G'GATC_C Cuts at: 2733 3013 3380 2733 Size: 280 367 5036 Fragments arranged by size: 5036 367 280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 3004 4459 618 Size: 325 217 627 891 326 1455 1842 Fragments arranged by size: 1842 1455 891 627 326 325 217 BanII G_rGCy'C Cuts at: 1391 3002 1391 Size: 1611 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2701 928 Size: 971 235 567 3910 Fragments arranged by size: 3910 971 567 235 BbvCI CC'TCA_GC Cuts at: 2745 2897 2745 Size: 152 5531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2181 1548 Size: 633 5050 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2302 5678 2302 Size: 3376 2307 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2248 3744 4900 1790 Size: 458 1496 1156 2573 Fragments arranged by size: 2573 1496 1156 458 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4343 1239 Size: 3104 2579 Bpu10I CC'TnA_GC Cuts at: 2745 2897 2745 Size: 152 5531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 4346 882 Size: 617 756 2091 2219 Fragments arranged by size: 2219 2091 756 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 2060 5677 2060 Size: 3617 2066 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5683 BseYI C'CCAG_C Cuts at: 2674 4886 5382 2674 Size: 2212 496 2975 Fragments arranged by size: 2975 2212 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2517 1163 Size: 573 781 4329 Fragments arranged by size: 4329 781 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2248 3744 3829 4900 2248 Size: 1496 85 1071 3031 Fragments arranged by size: 3031 1496 1071 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2248 3002 3744 3829 4900 1391 Size: 399 458 754 742 85 1071 2174 Fragments arranged by size: 2174 1071 754 742 458 399 85 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2162 2273 3038 844 Size: 1318 111 765 3489 Fragments arranged by size: 3489 1318 765 111 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 2119 96 Size: 2023 3660 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2302 2733 3013 3380 3765 3782 5234 5320 Size: 330 431 280 367 385 17 1452 86 Cuts at: 5320 5332 5678 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 431 385 367 346 330 280 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2729 2845 2918 2729 Size: 116 73 5494 Fragments arranged by size: 5494 116 73 BtgI C'CryG_G Cuts at: 513 1065 2004 2790 513 Size: 552 939 786 3406 Fragments arranged by size: 3406 939 786 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2450 2499 3199 3993 4013 2450 Size: 49 700 794 20 4120 Fragments arranged by size: 4120 794 700 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2310 2424 3130 Size: 54 1425 50 275 18 480 114 706 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 706 480 275 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 3613 1353 Size: 887 1373 3423 Fragments arranged by size: 3423 1373 887 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2724 3746 4343 5130 5398 1239 Size: 393 467 625 1022 597 787 268 1524 Fragments arranged by size: 1524 1022 787 625 597 467 393 268 EcoO109I rG'GnC_Cy Cuts at: 2211 2211 Size: 5683 EcoRI G'AATT_C Cuts at: 2084 2639 2992 3370 2084 Size: 555 353 378 4397 Fragments arranged by size: 4397 555 378 353 EcoRV GAT'ATC Cuts at: 2447 2447 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373 Size: 32 135 32 2752 32 42 32 2626 Fragments arranged by size: 2752 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 2682 3008 2682 Size: 326 5357 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 2426 10 Size: 54 1425 343 480 114 3267 Fragments arranged by size: 3267 1425 480 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2402 3641 4000 4159 518 Size: 648 1059 177 1239 359 159 2042 Fragments arranged by size: 2042 1239 1059 648 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2744 3099 3776 4924 5169 1606 Size: 1138 355 677 1148 245 2120 Fragments arranged by size: 2120 1148 1138 677 355 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 2592 387 Size: 2205 3478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2120 3041 4586 1132 Size: 97 891 921 1545 2229 Fragments arranged by size: 2229 1545 921 891 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 2116 4582 2116 Size: 2466 3217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 1108 Size: 1488 4195 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2211 2211 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 2282 2705 1613 Size: 669 423 4591 Fragments arranged by size: 4591 669 423 PsiI TTA'TAA Cuts at: 2648 2798 3253 2648 Size: 150 455 5078 Fragments arranged by size: 5078 455 150 PstI C_TGCA'G Cuts at: 830 2506 2561 3035 830 Size: 1676 55 474 3478 Fragments arranged by size: 3478 1676 474 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2744 2744 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2502 2557 3031 4166 4847 5038 826 Size: 219 1457 55 474 1135 681 191 1471 Fragments arranged by size: 1471 1457 1135 681 474 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4438 StyI C'CwwG_G Cuts at: 513 1065 2004 2313 3122 513 Size: 552 939 309 809 3074 Fragments arranged by size: 3074 939 809 552 309 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 3710 3727 3880 4065 4510 325 Size: 1255 611 1519 17 153 185 445 1498 Fragments arranged by size: 1519 1498 1255 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2119 3929 5641 Size: 274 80 33 51 1443 142 1810 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1810 1712 1443 274 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 5643 5675 2469 Size: 32 3142 32 2477 Fragments arranged by size: 3142 2477 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 2084 2088 2326 2762 3812 2084 Size: 4 238 436 1050 3955 Fragments arranged by size: 3955 1050 436 238 4 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI