################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HinfI HpaI HpaII KasI KpnI KspI MaeII MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 6 GCGG CCGC(-3/-1) AflII 777 CTTAAG C^TTAAG AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 70 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 158 GGGCCC GGGCC^C Asp700I 920 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 615 GGTCC G^G[AT]CC BalI 59 TGGCCA TGG^CCA BanI 118 GGCACC G^G[CT][GA]CC BanII 158 GGGCCC G[GA]GC[CT]^C BbsI 267 GAAGAC GAAGAC(2/6) BciVI 826 GTATCC GTATCC(6/5) BfaI 430 CTAG C^TAG BfrI 777 CTTAAG C^TTAAG BpuAI 267 GAAGAC GAAGAC(2/6) BsaBI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 16 GAGAC GTCTC(1/5) Bsp1407I 607 TGTACA T^GTACA BsrBI 4 GAGCGG CCGCTC(-3/-3) BsrDI 540 CATTGC GCAATG(2/0) BsrGI 607 TGTACA T^GTACA BsrI 693 ACTGG ACTGG(1/-1) BstAPI 69 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 390 GCGC GCG^C EaeI 59 TGGCCA [CT]^GGCC[GA] Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 313 CTCTTC CTCTTC(1/4) FauI 6 GCGGG CCCGC(4/6) HhaI 390 GCGC GCG^C HinP1I 390 GCGC G^CGC HincII 247 GTTGAC GT[CT]^[GA]AC HindII 247 GTTGAC GT[CT]^[GA]AC HindIII 707 AAGCTT A^AGCTT Ksp632I 313 CTCTTC CTCTTC(1/4) MaeI 430 CTAG C^TAG MamI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 59 TGGCCA TGG^CCA MscI 59 TGGCCA TGG^CCA MseI 778 TTAA T^TAA MspA1I 73 CAGCTG C[AC]G^C[GT]G PstI 67 CTGCAG CTGCA^G PvuII 73 CAGCTG CAG^CTG SfcI 67 CTGCAG C^T[GA][CT]AG SpeI 429 ACTAGT A^CTAGT SspBI 607 TGTACA T^GTACA StuI 781 AGGCCT AGG^CCT Tru9I 778 TTAA T^TAA XmnI 920 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BmyI 117 GGGCAC G[AGT]GC[ACT]^C 158 GGGCCC BseRI 97 CTCCTC GAGGAG(10/8) 798 CTCCTC Bsp1286I 117 GGGCAC G[AGT]GC[ACT]^C 158 GGGCCC BspMI 251 ACCTGC ACCTGC(4/8) 494 ACCTGC BstXI 143 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 236 CCAAGATGCTGG Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC 558 GCTGGC DraII 157 AGGGCCC [GA]G^G[ACGT]CC[CT] 158 GGGCCCT EcoO109I 157 AGGGCCC [GA]G^G[ACGT]CC[CT] 158 GGGCCCT EcoT14I 318 CCTTGG C^C[AT][AT]GG 482 CCTTGG MslI 294 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 594 CATCTTCATG NlaIV 118 GGCACC GG[ACGT]^[ACGT]CC 158 GGGCCC StyI 318 CCTTGG C^C[AT][AT]GG 482 CCTTGG Tsp45I 536 GTGAC ^GT[GC]AC 742 GTCAC Tsp509I 909 AATT ^AATT 924 AATT XcmI 140 CCACCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 272 CCATTTCTTTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TseI TspRI