Restriction map for
pFN21AE2237
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BbrPI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HindIII KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 6 GACGTC GACGT^C
AcsI 61 AAATTT [GA]^AATT[CT]
AocI 1556 CCTCAGG CC^T[ACGT]AGG
ApoI 61 AAATTT [GA]^AATT[CT]
AviII 1973 TGCGCA TGC^GCA
BbeI 1234 GGCGCC GGCGC^C
BbsI 1389 GTCTTC GAAGAC(2/6)
BcgI 471 GCAGTTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 149 CCCGG CC^[GC]GG
BglI 1614 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1389 GTCTTC GAAGAC(2/6)
BsaBI 132 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 206 GGTCTC GGTCTC(1/5)
BsmBI 106 GAGACG CGTCTC(1/5)
BstEII 1445 GGTTACC G^GT[ACGT]ACC
BstPI 1445 GGTTACC G^GT[ACGT]ACC
Bsu36I 1556 CCTCAGG CC^T[ACGT]AGG
DraIII 1172 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1556 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1445 GGTTACC G^GT[ACGT]ACC
FspI 1973 TGCGCA TGC^GCA
HaeII 1234 GGCGCC [GA]GCGC^[CT]
HincII 220 GTTAAC GT[CT]^[GA]AC
HindII 220 GTTAAC GT[CT]^[GA]AC
HpaI 220 GTTAAC GTT^AAC
KasI 1234 GGCGCC G^GCGCC
MamI 132 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1234 GGCGCC GG^CGCC
NciI 149 CCCGG CC^[GC]GG
NspI 2006 ACATGC [GA]CATG^[CT]
PpuMI 1336 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 722 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 189 GAGCTC GAGCT^C
SfoI 1234 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 56 CGCG CG^CG
1314 CGCG
AlwNI 1345 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1931 CAGCAGCTG
BamHI 1719 GGATCC G^GATCC
1785 GGATCC
BanI 296 GGCACC G^G[CT][GA]CC
1234 GGCGCC
BbvCI 1075 CCTCAGC CCTCAGC(-5/-2)
1293 CCTCAGC
BciVI 555 GGATAC GTATCC(6/5)
1416 GGATAC
BsrFI 1643 ACCGGC [GA]^CCGG[CT]
2069 ACCGGC
BstUI 56 CGCG CG^CG
1314 CGCG
BstXI 863 CCACCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1151 CCACCTCCCTGG
Cfr10I 1643 ACCGGC [GA]^CCGG[CT]
2069 ACCGGC
MaeII 7 ACGT A^CGT
600 ACGT
MvnI 56 CGCG CG^CG
1314 CGCG
PflMI 300 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1768 CCAATGGGTGG
PvuII 1348 CAGCTG CAG^CTG
1934 CAGCTG
TfiI 277 GATTC G^A[AT]TC
1671 GAATC
Van91I 300 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1768 CCAATGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII