(Circular) (Six-base) MAPSORT of: pFN21AE2237.seq Check: 4001 from: 1 to: 6784 pFN21AE2237 6784 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2812 2812 Size: 6784 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2015 4594 278 Size: 53 83 186 936 479 2579 2468 Fragments arranged by size: 2579 2468 936 479 186 83 53 AccI GT'mk_AC Cuts at: 1937 4127 1937 Size: 2190 4594 Acc65I G'GTAC_C Cuts at: 4105 4105 Size: 6784 AclI AA'CG_TT Cuts at: 1563 4911 5284 1563 Size: 3348 373 3063 Fragments arranged by size: 3348 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 2904 3077 3762 4847 6231 6499 1632 Size: 1073 199 173 685 1085 1384 268 1917 Fragments arranged by size: 1917 1384 1085 1073 685 268 199 173 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6587 AflIII A'CryG_T Cuts at: 1203 5683 6660 1203 Size: 4480 977 1327 Fragments arranged by size: 4480 1327 977 AgeI A'CCGG_T Cuts at: 5615 5615 Size: 6784 AhdI GACnn_n'nnGTC Cuts at: 5513 5513 Size: 6784 AleI CACnn'nnGTG Cuts at: 1166 3288 1166 Size: 2122 4662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6784 AlwNI CAG_nnn'CTG Cuts at: 3355 3941 4527 6099 3355 Size: 586 586 1572 4040 Fragments arranged by size: 4040 1572 586 586 ApaI G_GGCC'C Cuts at: 2984 3326 3621 2984 Size: 342 295 6147 Fragments arranged by size: 6147 342 295 ApaLI G'TGCA_C Cuts at: 4841 5997 4841 Size: 1156 5628 ApoI r'AATT_y Cuts at: 1493 2066 4093 4319 4471 6580 1493 Size: 573 2027 226 152 2109 1697 Fragments arranged by size: 2109 2027 1697 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 5338 160 Size: 872 4306 1606 Fragments arranged by size: 4306 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 AvaI C'yCGr_G Cuts at: 1870 1943 4109 1870 Size: 73 2166 4545 Fragments arranged by size: 4545 2166 73 BamHI G'GATC_C Cuts at: 3724 3790 4114 4481 3724 Size: 66 324 367 6027 Fragments arranged by size: 6027 367 324 66 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 3239 4105 5560 618 Size: 325 217 627 514 938 866 1455 1842 Fragments arranged by size: 1842 1455 938 866 627 514 325 217 BanII G_rGCy'C Cuts at: 1391 2198 2264 2825 2984 3101 3326 3621 3704 Size: 807 66 561 159 117 225 295 83 Cuts at: 3704 4103 1391 Size: 399 4072 Fragments arranged by size: 4072 807 561 399 295 225 159 117 83 66 BbeI G_GCGC'C Cuts at: 3243 3243 Size: 6784 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3387 928 Size: 971 1488 4325 Fragments arranged by size: 4325 1488 971 BbvCI CC'TCA_GC Cuts at: 3081 3299 3081 Size: 218 6566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2465 2499 4975 5009 1903 Size: 34 528 34 2476 34 3678 Fragments arranged by size: 3678 2476 528 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2554 3415 4676 5886 2554 Size: 861 1261 1210 3452 Fragments arranged by size: 3452 1261 1210 861 BclI T'GATC_A Cuts at: 1548 1548 Size: 6784 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3625 5395 136 Size: 107 122 71 631 605 1953 1770 1525 Fragments arranged by size: 1953 1770 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6779 6779 Size: 6784 BlpI GC'TnA_GC Cuts at: 4201 4201 Size: 6784 Bme1580I G_kGCm'C Cuts at: 1790 2984 3297 3326 3621 4033 4845 6001 1790 Size: 1194 313 29 295 412 812 1156 2573 Fragments arranged by size: 2573 1194 1156 812 412 313 295 29 BmgBI CAC'GTC Cuts at: 1318 2606 1318 Size: 1288 5496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2544 3615 4001 Size: 416 53 83 399 24 1127 1071 386 Cuts at: 4001 4515 5473 442 Size: 514 958 1753 Fragments arranged by size: 1753 1127 1071 958 514 416 399 386 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6784 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3096 3096 Size: 6784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3044 3701 3861 5444 1239 Size: 1805 657 160 1583 2579 Fragments arranged by size: 2579 1805 1583 657 160 Bpu10I CC'TnA_GC Cuts at: 3044 3071 3081 3299 3841 3044 Size: 27 10 218 542 5987 Fragments arranged by size: 5987 542 218 27 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4909 5774 6072 4909 Size: 865 298 5621 Fragments arranged by size: 5621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2217 5447 882 Size: 617 718 3230 2219 Fragments arranged by size: 3230 2219 718 617 BsaAI yAC'GTr Cuts at: 493 1204 4575 493 Size: 711 3371 2702 Fragments arranged by size: 3371 2702 711 BsaBI GATnn'nnATC Cuts at: 2141 6778 2141 Size: 4637 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3240 3333 3411 Size: 53 83 186 936 479 1228 93 78 Cuts at: 3411 4591 4973 5608 275 Size: 1180 382 635 1451 Fragments arranged by size: 1451 1228 1180 936 635 479 382 186 93 83 78 53 BsaWI w'CCGG_w Cuts at: 5217 5615 5889 6036 6447 5217 Size: 398 274 147 411 5554 Fragments arranged by size: 5554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 2909 2939 3211 Size: 30 165 30 729 30 747 30 272 Cuts at: 3211 3241 1178 Size: 30 4721 Fragments arranged by size: 4721 747 729 272 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2141 2403 2669 3118 3187 3190 3193 Size: 937 18 262 266 449 69 3 3 Cuts at: 3193 3202 3205 3862 4070 1186 Size: 9 3 657 208 3900 Fragments arranged by size: 3900 937 657 449 266 262 208 69 18 9 3 3 3 BseYI C'CCAG_C Cuts at: 2585 2597 3087 3325 3495 5987 6483 2585 Size: 12 490 238 170 2492 496 2886 Fragments arranged by size: 2886 2492 496 490 238 170 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2481 2831 3575 1163 Size: 573 609 136 350 744 4372 Fragments arranged by size: 4372 744 609 573 350 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4234 4995 6023 1540 Size: 275 186 2233 761 1028 2301 Fragments arranged by size: 2301 2233 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2198 2297 3187 4845 4930 6001 2198 Size: 99 890 1658 85 1071 2981 Fragments arranged by size: 2981 1658 1071 890 99 85 BsmI GAATG_Cn' Cuts at: 4295 4388 4295 Size: 93 6691 BsmBI CGTCTCn'nnnn_ Cuts at: 2105 2105 Size: 6784 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2264 2297 2825 2984 3101 3187 Size: 399 408 66 33 528 159 117 86 Cuts at: 3187 3297 3326 3621 3704 4033 4103 4845 4930 Size: 110 29 295 83 329 70 742 85 Cuts at: 4930 6001 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 528 408 399 329 295 159 117 110 86 85 83 70 66 33 29 BspEI T'CCGG_A Cuts at: 6447 6447 Size: 6784 BspHI T'CATG_A Cuts at: 4673 6403 4673 Size: 1730 5054 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2653 2812 2853 4139 844 Size: 1809 159 41 1286 3489 Fragments arranged by size: 3489 1809 1286 159 41 BsrBI CCG'CTC Cuts at: 1394 1823 4671 1394 Size: 429 2848 3507 Fragments arranged by size: 3507 2848 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5279 5453 65 Size: 1183 4031 174 1396 Fragments arranged by size: 4031 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3648 4074 5428 5615 1700 Size: 110 1838 426 1354 187 2869 Fragments arranged by size: 2869 1838 1354 426 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6784 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6784 BssSI C'ACGA_G Cuts at: 4844 5856 4844 Size: 1012 5772 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6784 BstBI TT'CG_AA Cuts at: 5588 5588 Size: 6784 BstEII G'GTnAC_C Cuts at: 1184 3450 1184 Size: 2266 4518 BstXI CCAn_nnnn'nTGG Cuts at: 2875 3163 2875 Size: 288 6496 BstYI r'GATC_y Cuts at: 1972 2332 3724 3790 4114 4481 4866 4883 6335 Size: 360 1392 66 324 367 385 17 1452 Cuts at: 6335 6421 6433 6779 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1392 385 367 360 346 324 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3562 3562 Size: 6784 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2305 2690 3835 513 Size: 552 939 36 265 385 1145 3462 Fragments arranged by size: 3462 1145 939 552 385 265 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6045 Fragments arranged by size: 6045 683 56 BtsI GCAGTG_nn' Cuts at: 2861 4075 4300 5094 5114 2861 Size: 1214 225 794 20 4531 Fragments arranged by size: 4531 1214 794 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6784 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6749 DraI TTT'AAA Cuts at: 4088 4088 Size: 6784 DraIII CAC_nnn'GTG Cuts at: 3182 3182 Size: 6784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5791 809 Size: 507 4475 1802 Fragments arranged by size: 4475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2247 3650 Size: 54 1425 50 275 18 207 210 1403 Cuts at: 3650 3909 4231 5120 6749 8 Size: 259 322 889 1629 43 Fragments arranged by size: 1629 1425 1403 889 322 275 259 210 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4231 1537 Size: 275 2419 4090 Fragments arranged by size: 4090 2419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2725 2880 3211 4714 1353 Size: 1372 155 331 1503 3423 Fragments arranged by size: 3423 1503 1372 331 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5371 5745 5891 387 Size: 1064 138 3782 374 146 1280 Fragments arranged by size: 3782 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2196 2196 Size: 6784 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2705 2904 3044 3077 3701 3762 3861 Size: 393 1073 199 140 33 624 61 99 Cuts at: 3861 4847 5444 6231 6499 1239 Size: 986 597 787 268 1524 Fragments arranged by size: 1524 1073 986 787 624 597 393 268 199 140 99 61 33 EcoO109I rG'GnC_Cy Cuts at: 2573 3270 3342 3746 3845 2573 Size: 697 72 404 99 5512 Fragments arranged by size: 5512 697 404 99 72 EcoRI G'AATT_C Cuts at: 4093 4471 4093 Size: 378 6406 FspI TGC'GCA Cuts at: 3980 5290 3980 Size: 1310 5474 HaeII r_GCGC'y Cuts at: 1678 3243 5931 1678 Size: 1565 2688 2531 Fragments arranged by size: 2688 2531 1565 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 3689 3721 5425 Size: 32 135 32 560 32 1525 32 1704 Cuts at: 5425 5457 5499 5531 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1704 1525 560 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 4128 4374 669 Size: 988 281 289 1901 246 3079 Fragments arranged by size: 3079 1901 988 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6784 HpaI GTT'AAC Cuts at: 2227 4374 2227 Size: 2147 4637 KasI G'GCGC_C Cuts at: 3239 3239 Size: 6784 KpnI G_GTAC'C Cuts at: 4109 4109 Size: 6784 MfeI C'AATT_G Cuts at: 4383 4383 Size: 6784 MluI A'CGCG_T Cuts at: 6660 6660 Size: 6784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5898 6082 1305 Size: 4593 184 2007 Fragments arranged by size: 4593 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4962 Fragments arranged by size: 4962 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2205 2535 3288 4742 5101 5260 518 Size: 648 1039 330 753 1454 359 159 2042 Fragments arranged by size: 2042 1454 1039 753 648 359 330 159 MspA1I CmG'CkG Cuts at: 1606 3355 3448 3941 4200 4877 6025 6270 1606 Size: 1749 93 493 259 677 1148 245 2120 Fragments arranged by size: 2120 1749 1148 677 493 259 245 93 NarI GG'CG_CC Cuts at: 3240 3240 Size: 6784 NcoI C'CATG_G Cuts at: 513 1065 2004 2305 2690 3835 513 Size: 552 939 301 385 1145 3462 Fragments arranged by size: 3462 1145 939 552 385 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 6784 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6784 NotI GC'GGCC_GC Cuts at: 4231 4231 Size: 6784 NspI r_CATG'y Cuts at: 1132 1229 4015 4142 5687 1132 Size: 97 2786 127 1545 2229 Fragments arranged by size: 2786 2229 1545 127 97 PasI CC'CwG_GG Cuts at: 1348 3163 3266 1348 Size: 1815 103 4866 Fragments arranged by size: 4866 1815 103 PciI A'CATG_T Cuts at: 5683 5683 Size: 6784 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 3779 1108 Size: 1203 1468 4113 Fragments arranged by size: 4113 1468 1203 PmeI GTTT'AAAC Cuts at: 4088 4088 Size: 6784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4853 4885 6366 6398 4853 Size: 32 1481 32 5239 Fragments arranged by size: 5239 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3342 3342 Size: 6784 PshAI GACnn'nnGTC Cuts at: 1613 2731 1613 Size: 1118 5666 PsiI TTA'TAA Cuts at: 4354 4354 Size: 6784 PspOMI G'GGCC_C Cuts at: 2980 3322 3617 2980 Size: 342 295 6147 Fragments arranged by size: 6147 342 295 PstI C_TGCA'G Cuts at: 830 2091 2467 3036 4136 830 Size: 1261 376 569 1100 3478 Fragments arranged by size: 3478 1261 1100 569 376 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6784 PvuII CAG'CTG Cuts at: 3355 3941 3355 Size: 586 6198 SacI G_AGCT'C Cuts at: 2198 2198 Size: 6784 SalI G'TCGA_C Cuts at: 1936 4126 1936 Size: 2190 4594 SbfI CC_TGCA'GG Cuts at: 4136 4136 Size: 6784 ScaI AGT'ACT Cuts at: 5032 6744 5032 Size: 1712 5072 SfcI C'TryA_G Cuts at: 826 1045 2087 2392 2463 3032 3518 4132 5267 Size: 219 1042 305 71 569 486 614 1135 Cuts at: 5267 5948 6139 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 614 569 486 305 219 191 71 SfoI GGC'GCC Cuts at: 3241 3241 Size: 6784 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6784 SmaI CCC'GGG Cuts at: 1872 4111 1872 Size: 2239 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4888 5789 6051 820 Size: 197 926 2945 901 262 1553 Fragments arranged by size: 2945 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4575 493 Size: 4082 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6784 SphI G_CATG'C Cuts at: 1132 4142 1132 Size: 3010 3774 SspI AAT'ATT Cuts at: 5 52 4708 5 Size: 47 4656 2081 Fragments arranged by size: 4656 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6784 StyI C'CwwG_G Cuts at: 513 1065 2004 2305 2690 3132 3835 4223 513 Size: 552 939 301 385 442 703 388 3074 Fragments arranged by size: 3074 939 703 552 442 388 385 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2721 3039 3400 3767 4811 4828 4981 Size: 1255 1141 318 361 367 1044 17 153 Cuts at: 4981 5166 5611 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1141 1044 445 367 361 318 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3145 5030 6742 Size: 274 80 33 51 1443 1168 1885 1712 Cuts at: 6742 96 Size: 138 Fragments arranged by size: 1885 1712 1443 1168 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3211 3243 6744 6776 3211 Size: 32 3501 32 3219 Fragments arranged by size: 3501 3219 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6784 XbaI T'CTAG_A Cuts at: 4120 4120 Size: 6784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2237 2427 2950 3160 3718 2237 Size: 190 523 210 558 5303 Fragments arranged by size: 5303 558 523 210 190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6784 XmaI C'CCGG_G Cuts at: 1870 4109 1870 Size: 2239 4545 XmnI GAAnn'nnTTC Cuts at: 4913 4913 Size: 6784 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2013 4592 276 Size: 53 83 186 936 479 2579 2468 Fragments arranged by size: 2579 2468 936 479 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI