Restriction map for
pFN21AE2237
(Linear) (Six-base) MAPSORT of: KIEE2237.seq Check: 4666 from: 1 to: 2079
KIEE2237.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 807 2079
Size: 807 1272
AatII G_ACGT'C
Cuts at: 0 10 2079
Size: 10 2069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 899 1072 1757 2079
Size: 700 199 173 685 322
Fragments arranged by size:
700 685 322 199 173
AleI CACnn'nnGTG
Cuts at: 0 1283 2079
Size: 1283 796
AlwNI CAG_nnn'CTG
Cuts at: 0 1350 1936 2079
Size: 1350 586 143
Fragments arranged by size:
1350 586 143
ApaI G_GGCC'C
Cuts at: 0 979 1321 1616 2079
Size: 979 342 295 463
Fragments arranged by size:
979 463 342 295
ApoI r'AATT_y
Cuts at: 0 61 2079
Size: 61 2018
BamHI G'GATC_C
Cuts at: 0 1719 1785 2079
Size: 1719 66 294
Fragments arranged by size:
1719 294 66
BanI G'GyrC_C
Cuts at: 0 296 1234 2079
Size: 296 938 845
Fragments arranged by size:
938 845 296
BanII G_rGCy'C
Cuts at: 0 193 259 820 979 1096 1321 1616 1699
Size: 193 66 561 159 117 225 295 83
Cuts at: 1699 2079
Size: 380
Fragments arranged by size:
561 380 295 225 193 159 117 83
66
BbeI G_GCGC'C
Cuts at: 0 1238 2079
Size: 1238 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 1382 2079
Size: 1382 697
BbvCI CC'TCA_GC
Cuts at: 0 1076 1294 2079
Size: 1076 218 785
Fragments arranged by size:
1076 785 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 494 2079
Size: 460 34 1585
Fragments arranged by size:
1585 460 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 549 1410 2079
Size: 549 861 669
Fragments arranged by size:
861 669 549
BglI GCCn_nnn'nGGC
Cuts at: 0 1620 2079
Size: 1620 459
Bme1580I G_kGCm'C
Cuts at: 0 979 1292 1321 1616 2028 2079
Size: 979 313 29 295 412 51
Fragments arranged by size:
979 412 313 295 51 29
BmgBI CAC'GTC
Cuts at: 0 601 2079
Size: 601 1478
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 1610 1996 2079
Size: 539 1071 386 83
Fragments arranged by size:
1071 539 386 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1091 2079
Size: 1091 988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1696 1856 2079
Size: 1039 657 160 223
Fragments arranged by size:
1039 657 223 160
Bpu10I CC'TnA_GC
Cuts at: 0 1039 1066 1076 1294 1836 2079
Size: 1039 27 10 218 542 243
Fragments arranged by size:
1039 542 243 218 27 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 212 2079
Size: 212 1867
BsaBI GATnn'nnATC
Cuts at: 0 136 2079
Size: 136 1943
BsaHI Gr'CG_yC
Cuts at: 0 7 1235 1328 1406 2079
Size: 7 1228 93 78 673
Fragments arranged by size:
1228 673 93 78 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 904 934 1206 1236 2079
Size: 127 30 747 30 272 30 843
Fragments arranged by size:
843 747 272 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 136 398 664 1113 1182 1185 1188
Size: 118 18 262 266 449 69 3 3
Cuts at: 1188 1197 1200 1857 2065 2079
Size: 9 3 657 208 14
Fragments arranged by size:
657 449 266 262 208 118 69 18
14 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 580 592 1082 1320 1490 2079
Size: 580 12 490 238 170 589
Fragments arranged by size:
589 580 490 238 170 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 476 826 1570 2079
Size: 340 136 350 744 509
Fragments arranged by size:
744 509 350 340 136
BsiHKAI G_wGCw'C
Cuts at: 0 193 292 1182 2079
Size: 193 99 890 897
Fragments arranged by size:
897 890 193 99
BsmBI CGTCTCn'nnnn_
Cuts at: 0 100 2079
Size: 100 1979
Bsp1286I G_dGCh'C
Cuts at: 0 193 259 292 820 979 1096 1182 1292
Size: 193 66 33 528 159 117 86 110
Cuts at: 1292 1321 1616 1699 2028 2079
Size: 29 295 83 329 51
Fragments arranged by size:
528 329 295 193 159 117 110 86
83 66 51 33 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 807 848 2079
Size: 648 159 41 1231
Fragments arranged by size:
1231 648 159 41
BsrFI r'CCGG_y
Cuts at: 0 1643 2069 2079
Size: 1643 426 10
Fragments arranged by size:
1643 426 10
BstEII G'GTnAC_C
Cuts at: 0 1445 2079
Size: 1445 634
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1158 2079
Size: 870 288 921
Fragments arranged by size:
921 870 288
BstYI r'GATC_y
Cuts at: 0 327 1719 1785 2079
Size: 327 1392 66 294
Fragments arranged by size:
1392 327 294 66
Bsu36I CC'TnA_GG
Cuts at: 0 1557 2079
Size: 1557 522
BtgI C'CryG_G
Cuts at: 0 35 300 685 1830 2079
Size: 35 265 385 1145 249
Fragments arranged by size:
1145 385 265 249 35
BtsI GCAGTG_nn'
Cuts at: 0 856 2070 2079
Size: 856 1214 9
Fragments arranged by size:
1214 856 9
DraIII CAC_nnn'GTG
Cuts at: 0 1177 2079
Size: 1177 902
EaeI y'GGCC_r
Cuts at: 0 32 242 1645 1904 2079
Size: 32 210 1403 259 175
Fragments arranged by size:
1403 259 210 175 32
EarI CTCTTCn'nnn_
Cuts at: 0 720 875 1206 2079
Size: 720 155 331 873
Fragments arranged by size:
873 720 331 155
EcoICRI GAG'CTC
Cuts at: 0 191 2079
Size: 191 1888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 899 1039 1072 1696 1757 1856 2079
Size: 700 199 140 33 624 61 99 223
Fragments arranged by size:
700 624 223 199 140 99 61 33
EcoO109I rG'GnC_Cy
Cuts at: 0 568 1265 1337 1741 1840 2079
Size: 568 697 72 404 99 239
Fragments arranged by size:
697 568 404 239 99 72
FspI TGC'GCA
Cuts at: 0 1975 2079
Size: 1975 104
HaeII r_GCGC'y
Cuts at: 0 1238 2079
Size: 1238 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 1684 1716 2079
Size: 127 32 1525 32 363
Fragments arranged by size:
1525 363 127 32 32
HincII GTy'rAC
Cuts at: 0 222 2079
Size: 222 1857
HpaI GTT'AAC
Cuts at: 0 222 2079
Size: 222 1857
KasI G'GCGC_C
Cuts at: 0 1234 2079
Size: 1234 845
MslI CAynn'nnrTG
Cuts at: 0 200 530 1283 2079
Size: 200 330 753 796
Fragments arranged by size:
796 753 330 200
MspA1I CmG'CkG
Cuts at: 0 1350 1443 1936 2079
Size: 1350 93 493 143
Fragments arranged by size:
1350 493 143 93
NarI GG'CG_CC
Cuts at: 0 1235 2079
Size: 1235 844
NcoI C'CATG_G
Cuts at: 0 300 685 1830 2079
Size: 300 385 1145 249
Fragments arranged by size:
1145 385 300 249
NspI r_CATG'y
Cuts at: 0 2010 2079
Size: 2010 69
PasI CC'CwG_GG
Cuts at: 0 1158 1261 2079
Size: 1158 103 818
Fragments arranged by size:
1158 818 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 1774 2079
Size: 306 1468 305
Fragments arranged by size:
1468 306 305
PpuMI rG'GwC_Cy
Cuts at: 0 1337 2079
Size: 1337 742
PshAI GACnn'nnGTC
Cuts at: 0 726 2079
Size: 726 1353
PspOMI G'GGCC_C
Cuts at: 0 975 1317 1612 2079
Size: 975 342 295 467
Fragments arranged by size:
975 467 342 295
PstI C_TGCA'G
Cuts at: 0 86 462 1031 2079
Size: 86 376 569 1048
Fragments arranged by size:
1048 569 376 86
PvuII CAG'CTG
Cuts at: 0 1350 1936 2079
Size: 1350 586 143
Fragments arranged by size:
1350 586 143
SacI G_AGCT'C
Cuts at: 0 193 2079
Size: 193 1886
SfcI C'TryA_G
Cuts at: 0 82 387 458 1027 1513 2079
Size: 82 305 71 569 486 566
Fragments arranged by size:
569 566 486 305 82 71
SfoI GGC'GCC
Cuts at: 0 1236 2079
Size: 1236 843
StyI C'CwwG_G
Cuts at: 0 300 685 1127 1830 2079
Size: 300 385 442 703 249
Fragments arranged by size:
703 442 385 300 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 716 1034 1395 1762 2079
Size: 716 318 361 367 317
Fragments arranged by size:
716 367 361 318 317
TatI w'GTAC_w
Cuts at: 0 1140 2079
Size: 1140 939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1206 1238 2079
Size: 1206 32 841
Fragments arranged by size:
1206 841 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 232 422 945 1155 1713 2079
Size: 232 190 523 210 558 366
Fragments arranged by size:
558 523 366 232 210 190
ZraI GAC'GTC
Cuts at: 0 8 2079
Size: 8 2071
Enzymes that do cut:
AarI AatII AcuI AleI AlwNI ApaI ApoI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I HincII HpaI KasI MslI MspA1I NarI NcoI
NspI PasI PflMI PpuMI PshAI PspOMI PstI PvuII
SacI SfcI SfoI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BclI BfrBI BglII BlpI BmtI BpuEI BsaAI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI