Restriction map for
pFN21AE1855
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AflIII AgeI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspI AvaI AviII AvrII
BalI BamHI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstYI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1010 GGTACC G^GTACC
AccI 944 GTATAC GT^[AC][GT]AC
AcyI 844 GGCGCC G[GA]^CG[CT]C
Alw44I 412 GTGCAC G^TGCAC
AlwI 987 GATCC GGATC(4/5)
ApaLI 412 GTGCAC G^TGCAC
Asp718I 1010 GGTACC G^GTACC
AspHI 412 GTGCAC G[AT]GC[AT]^C
BanII 94 GGGCTC G[GA]GC[CT]^C
BbeI 844 GGCGCC GGCGC^C
BcgI 900 CGATGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 967 CTAG C^TAG
BsaHI 844 GGCGCC G[GA]^CG[CT]C
BseRI 882 CTCCTC GAGGAG(10/8)
BsiHKAI 412 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1082 TGTACA T^GTACA
BsrDI 906 CATTGC GCAATG(2/0)
BsrGI 1082 TGTACA T^GTACA
Bst1107I 944 GTATAC GTA^TAC
BstXI 246 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 944 GTATAC GTA^TAC
CfoI 845 GCGC GCG^C
DdeI 464 CTAAG C^T[ACGT]AG
DpnII 987 GATC ^GATC
DraII 730 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 500 CGGCCA [CT]^GGCC[GA]
EcoNI 615 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 730 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 297 GCGGG CCCGC(4/6)
HaeII 844 GGCGCC [GA]GCGC^[CT]
HhaI 845 GCGC GCG^C
Hin1I 844 GGCGCC G[GA]^CG[CT]C
HinP1I 845 GCGC G^CGC
KasI 844 GGCGCC G^GCGCC
KpnI 1010 GGTACC GGTAC^C
MaeI 967 CTAG C^TAG
MaeIII 266 GTCAC ^GT[ACGT]AC
MboI 987 GATC ^GATC
MspA1I 211 CAGCTG C[AC]G^C[GT]G
NarI 844 GGCGCC GG^CGCC
NdeII 987 GATC ^GATC
PpuMI 730 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 211 CAGCTG CAG^CTG
Sau3AI 987 GATC ^GATC
SfaNI 707 GCATC GCATC(5/9)
SfoI 844 GGCGCC GGC^GCC
SmlI 799 CTCAAG C^T[CT][GA]AG
SspBI 1082 TGTACA T^GTACA
TfiI 52 GATTC G^A[AT]TC
Tsp45I 266 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 756 CCGC CCGC(-3/-1)
297 GCGG
AhdI 669 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
772 GACATCCAGTC
AspEI 669 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
772 GACATCCAGTC
AvaII 731 GGACC G^G[AT]CC
765 GGTCC
BbsI 606 GAAGAC GAAGAC(2/6)
19 GTCTTC
BglI 493 GCCCTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
836 GCCAGGATGGC
BpuAI 606 GAAGAC GAAGAC(2/6)
19 GTCTTC
BspMI 733 ACCTGC ACCTGC(4/8)
1028 ACCTGC
DrdI 257 GACTGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
271 GACCAGACAGTC
Eam1105I 669 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
772 GACATCCAGTC
HphI 49 GGTGA GGTGA(8/7)
518 TCACC
MslI 219 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1042 CACAGTGATG
NcoI 503 CCATGG C^CATGG
998 CCATGG
PleI 687 GACTC GAGTC(4/5)
1017 GACTC
StuI 41 AGGCCT AGG^CCT
138 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwNI BanI BbvI
BcnI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfcI StyI
TseI TspRI XcmI