(Circular) (Six-base) MAPSORT of: pFN21AE1855.seq Check: 8403 from: 1 to: 5824 pFN21AE1855 5824 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2950 3167 1937 Size: 1013 217 4594 Fragments arranged by size: 4594 1013 217 Acc65I G'GTAC_C Cuts at: 3015 3145 3015 Size: 130 5694 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 3887 5271 5539 1632 Size: 1010 1245 1384 268 1917 Fragments arranged by size: 1917 1384 1245 1010 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 3121 4723 5700 1203 Size: 1918 1602 977 1327 Fragments arranged by size: 1918 1602 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 2679 2782 4553 2679 Size: 103 1771 3950 Fragments arranged by size: 3950 1771 103 AleI CACnn'nnGTG Cuts at: 1166 2228 1166 Size: 1062 4762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3055 3087 3055 Size: 32 5792 AlwNI CAG_nnn'CTG Cuts at: 2198 2299 3105 3567 5139 2198 Size: 101 806 462 1572 2883 Fragments arranged by size: 2883 1572 806 462 101 ApaLI G'TGCA_C Cuts at: 2417 3881 5037 2417 Size: 1464 1156 3204 Fragments arranged by size: 3204 1464 1156 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3149 1870 Size: 73 1206 4545 Fragments arranged by size: 4545 1206 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2849 2936 3015 3145 4600 Size: 325 217 627 1062 87 79 130 1455 Cuts at: 4600 618 Size: 1842 Fragments arranged by size: 1842 1455 1062 627 325 217 130 87 79 BanII G_rGCy'C Cuts at: 1391 2103 3143 1391 Size: 712 1040 4072 Fragments arranged by size: 4072 1040 712 BbeI G_GCGC'C Cuts at: 2853 2853 Size: 5824 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 2618 928 Size: 971 118 601 4134 Fragments arranged by size: 4134 971 601 118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2894 2928 4015 4049 1903 Size: 34 50 34 873 34 1087 34 3678 Fragments arranged by size: 3678 1087 873 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2504 2847 4435 Size: 107 122 71 631 605 832 343 1588 Cuts at: 4435 136 Size: 1525 Fragments arranged by size: 1588 1525 832 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2421 2939 3885 5041 1790 Size: 631 518 946 1156 2573 Fragments arranged by size: 2573 1156 946 631 518 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2087 2285 4484 1239 Size: 788 60 198 2199 2579 Fragments arranged by size: 2579 2199 788 198 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 3949 4814 5112 2789 Size: 1160 865 298 3501 Fragments arranged by size: 3501 1160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 2176 2666 4487 882 Size: 617 546 131 490 1821 2219 Fragments arranged by size: 2219 1821 617 546 490 131 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2850 3631 4013 4648 Size: 53 83 186 936 1317 781 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1317 936 781 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 3055 3085 1178 Size: 30 165 30 1023 30 599 30 3917 Fragments arranged by size: 3917 1023 599 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2878 1186 Size: 1692 4132 BseYI C'CCAG_C Cuts at: 2127 2486 2698 5027 5523 2127 Size: 359 212 2329 496 2428 Fragments arranged by size: 2428 2329 496 359 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2421 3885 3970 5041 2421 Size: 1464 85 1071 3204 Fragments arranged by size: 3204 1464 1071 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2421 2939 3143 3885 3970 5041 Size: 399 313 318 518 204 742 85 1071 Cuts at: 5041 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 518 399 318 313 204 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 3042 3179 844 Size: 1903 295 137 3489 Fragments arranged by size: 3489 1903 295 137 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2910 4319 4493 65 Size: 1183 1662 1409 174 1396 Fragments arranged by size: 1662 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 3087 96 Size: 2991 2833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2258 2258 Size: 5824 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2951 2951 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 2012 2166 2508 2648 3003 513 Size: 552 947 154 342 140 355 3334 Fragments arranged by size: 3334 947 552 355 342 154 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2724 3340 4134 4154 2724 Size: 616 794 20 4394 Fragments arranged by size: 4394 794 616 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2317 2352 529 Size: 35 1753 35 4001 Fragments arranged by size: 4001 1753 35 35 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2268 2282 4831 809 Size: 507 952 14 2549 1802 Fragments arranged by size: 2549 1802 952 507 14 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3271 4160 Size: 54 1425 50 275 18 675 766 889 Cuts at: 4160 5789 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 766 675 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2087 2285 2642 3887 4484 5271 Size: 393 395 60 198 357 1245 597 787 Cuts at: 5271 5539 1239 Size: 268 1524 Fragments arranged by size: 1524 1245 787 597 395 393 357 268 198 60 EcoNI CCTnn'n_nnAGG Cuts at: 2624 2624 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2736 2736 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2853 4971 1678 Size: 1175 2118 2531 Fragments arranged by size: 2531 2118 1175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2665 2697 2768 2800 4465 Size: 32 135 32 1093 32 71 32 1665 Cuts at: 4465 4497 4539 4571 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1665 1093 135 71 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KasI G'GCGC_C Cuts at: 2849 2849 Size: 5824 KpnI G_GTAC'C Cuts at: 3019 3149 3019 Size: 130 5694 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 3051 3782 4141 4300 518 Size: 648 1062 823 731 359 159 2042 Fragments arranged by size: 2042 1062 823 731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2218 3240 3917 5065 5310 1606 Size: 612 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 612 245 NarI GG'CG_CC Cuts at: 2850 2850 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2508 3003 513 Size: 552 1443 495 3334 Fragments arranged by size: 3334 1443 552 495 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 2446 2917 1348 Size: 1098 471 4255 Fragments arranged by size: 4255 1098 471 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PfoI T'CCnGG_A Cuts at: 2063 2772 2063 Size: 709 5115 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3087 3893 3925 5406 5438 3055 Size: 32 806 32 1481 32 3441 Fragments arranged by size: 3441 1481 806 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2736 2736 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 2826 3394 2826 Size: 568 5256 PstI C_TGCA'G Cuts at: 830 2210 2291 2313 2968 3176 830 Size: 1380 81 22 655 208 3478 Fragments arranged by size: 3478 1380 655 208 81 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2218 2218 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2206 2287 2309 2964 3172 4307 4988 Size: 219 1161 81 22 655 208 1135 681 Cuts at: 4988 5179 826 Size: 191 1471 Fragments arranged by size: 1471 1161 1135 681 655 219 208 191 81 22 SfoI GGC'GCC Cuts at: 2851 2851 Size: 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 3928 4829 5091 820 Size: 197 926 861 1124 901 262 1553 Fragments arranged by size: 1553 1124 926 901 861 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2048 2145 1517 Size: 531 97 5196 Fragments arranged by size: 5196 531 97 StyI C'CwwG_G Cuts at: 513 1065 2410 2425 2508 2881 3003 3263 513 Size: 552 1345 15 83 373 122 260 3074 Fragments arranged by size: 3074 1345 552 373 260 122 83 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2328 2438 2971 3851 3868 4021 4206 Size: 1255 748 110 533 880 17 153 185 Cuts at: 4206 4651 325 Size: 445 1498 Fragments arranged by size: 1498 1255 880 748 533 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3087 4070 5782 Size: 274 80 33 51 1443 1110 983 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1712 1443 1110 983 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2537 2569 5784 5816 2537 Size: 32 3215 32 2545 Fragments arranged by size: 3215 2545 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2333 2655 3034 2333 Size: 322 379 5123 Fragments arranged by size: 5123 379 322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI