Restriction map for
pFN21AE1855
(Linear) (Six-base) MAPSORT of: KIEE1855.seq Check: 1129 from: 1 to: 1119
KIEE1855.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 945 1119
Size: 945 174
Acc65I G'GTAC_C
Cuts at: 0 1010 1119
Size: 1010 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1119
Size: 637 482
AhdI GACnn_n'nnGTC
Cuts at: 0 674 777 1119
Size: 674 103 342
Fragments arranged by size:
674 342 103
AleI CACnn'nnGTG
Cuts at: 0 223 1119
Size: 223 896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1119
Size: 1050 32 37
Fragments arranged by size:
1050 37 32
AlwNI CAG_nnn'CTG
Cuts at: 0 193 294 1100 1119
Size: 193 101 806 19
Fragments arranged by size:
806 193 101 19
ApaLI G'TGCA_C
Cuts at: 0 412 1119
Size: 412 707
BanI G'GyrC_C
Cuts at: 0 844 931 1010 1119
Size: 844 87 79 109
Fragments arranged by size:
844 109 87 79
BanII G_rGCy'C
Cuts at: 0 98 1119
Size: 98 1021
BbeI G_GCGC'C
Cuts at: 0 848 1119
Size: 848 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 613 1119
Size: 12 601 506
Fragments arranged by size:
601 506 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 923 1119
Size: 889 34 196
Fragments arranged by size:
889 196 34
BglI GCCn_nnn'nGGC
Cuts at: 0 499 842 1119
Size: 499 343 277
Fragments arranged by size:
499 343 277
Bme1580I G_kGCm'C
Cuts at: 0 416 934 1119
Size: 416 518 185
Fragments arranged by size:
518 416 185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 1119
Size: 135 984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 82 280 1119
Size: 22 60 198 839
Fragments arranged by size:
839 198 60 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1119
Size: 784 335
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 171 661 1119
Size: 40 131 490 458
Fragments arranged by size:
490 458 131 40
BsaHI Gr'CG_yC
Cuts at: 0 845 1119
Size: 845 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 1050 1080 1119
Size: 421 30 599 30 39
Fragments arranged by size:
599 421 39 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 873 1119
Size: 873 246
BseYI C'CCAG_C
Cuts at: 0 122 481 693 1119
Size: 122 359 212 426
Fragments arranged by size:
426 359 212 122
BsiHKAI G_wGCw'C
Cuts at: 0 416 1119
Size: 416 703
Bsp1286I G_dGCh'C
Cuts at: 0 98 416 934 1119
Size: 98 318 518 185
Fragments arranged by size:
518 318 185 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 1037 1119
Size: 742 295 82
Fragments arranged by size:
742 295 82
BsrDI GCAATG_nn'
Cuts at: 0 905 1119
Size: 905 214
BsrGI T'GTAC_A
Cuts at: 0 1082 1119
Size: 1082 37
BstXI CCAn_nnnn'nTGG
Cuts at: 0 253 1119
Size: 253 866
BstZ17I GTA'TAC
Cuts at: 0 946 1119
Size: 946 173
BtgI C'CryG_G
Cuts at: 0 7 161 503 643 998 1119
Size: 7 154 342 140 355 121
Fragments arranged by size:
355 342 154 140 121 7
BtsI GCAGTG_nn'
Cuts at: 0 719 1119
Size: 719 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 312 347 1119
Size: 312 35 772
Fragments arranged by size:
772 312 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 263 277 1119
Size: 263 14 842
Fragments arranged by size:
842 263 14
EaeI y'GGCC_r
Cuts at: 0 500 1119
Size: 500 619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 82 280 637 1119
Size: 22 60 198 357 482
Fragments arranged by size:
482 357 198 60 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 1119
Size: 619 500
EcoO109I rG'GnC_Cy
Cuts at: 0 731 1119
Size: 731 388
HaeII r_GCGC'y
Cuts at: 0 848 1119
Size: 848 271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 660 692 763 795 1119
Size: 660 32 71 32 324
Fragments arranged by size:
660 324 71 32 32
KasI G'GCGC_C
Cuts at: 0 844 1119
Size: 844 275
KpnI G_GTAC'C
Cuts at: 0 1014 1119
Size: 1014 105
MslI CAynn'nnrTG
Cuts at: 0 223 1046 1119
Size: 223 823 73
Fragments arranged by size:
823 223 73
MspA1I CmG'CkG
Cuts at: 0 213 1119
Size: 213 906
NarI GG'CG_CC
Cuts at: 0 845 1119
Size: 845 274
NcoI C'CATG_G
Cuts at: 0 503 998 1119
Size: 503 495 121
Fragments arranged by size:
503 495 121
PasI CC'CwG_GG
Cuts at: 0 441 912 1119
Size: 441 471 207
Fragments arranged by size:
471 441 207
PfoI T'CCnGG_A
Cuts at: 0 58 767 1119
Size: 58 709 352
Fragments arranged by size:
709 352 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1119
Size: 1050 32 37
Fragments arranged by size:
1050 37 32
PpuMI rG'GwC_Cy
Cuts at: 0 731 1119
Size: 731 388
PsiI TTA'TAA
Cuts at: 0 821 1119
Size: 821 298
PstI C_TGCA'G
Cuts at: 0 205 286 308 963 1119
Size: 205 81 22 655 156
Fragments arranged by size:
655 205 156 81 22
PvuII CAG'CTG
Cuts at: 0 213 1119
Size: 213 906
SfcI C'TryA_G
Cuts at: 0 201 282 304 959 1119
Size: 201 81 22 655 160
Fragments arranged by size:
655 201 160 81 22
SfoI GGC'GCC
Cuts at: 0 846 1119
Size: 846 273
SmlI C'TyrA_G
Cuts at: 0 799 1119
Size: 799 320
StuI AGG'CCT
Cuts at: 0 43 140 1119
Size: 43 97 979
Fragments arranged by size:
979 97 43
StyI C'CwwG_G
Cuts at: 0 405 420 503 876 998 1119
Size: 405 15 83 373 122 121
Fragments arranged by size:
405 373 122 121 83 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 323 433 966 1119
Size: 323 110 533 153
Fragments arranged by size:
533 323 153 110
TatI w'GTAC_w
Cuts at: 0 1082 1119
Size: 1082 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 532 564 1119
Size: 532 32 555
Fragments arranged by size:
555 532 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 650 1029 1119
Size: 328 322 379 90
Fragments arranged by size:
379 328 322 90
Enzymes that do cut:
AccI Acc65I AcuI AhdI AleI AloI AlwNI ApaLI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BplI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrDI BsrGI BstXI BstZ17I BtgI BtsI
CspCI DrdI EaeI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI KpnI MslI MspA1I NarI NcoI PasI PfoI
PpiI PpuMI PsiI PstI PvuII SfcI SfoI SmlI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AlfI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI ClaI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI