Restriction map for
pFN21AB7606
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MslI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 446 TCCGGA T^CCGGA
AcyI 85 GGCGCC G[GA]^CG[CT]C
AfaI 562 GTAC GT^AC
AlwNI 4 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 439 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 324 CCTAGG C^CTAGG
BbeI 85 GGCGCC GGCGC^C
BfaI 325 CTAG C^TAG
BlnI 324 CCTAGG C^CTAGG
BsaAI 943 TACGTG [CT]AC^GT[GA]
BsaHI 85 GGCGCC G[GA]^CG[CT]C
BseAI 446 TCCGGA T^CCGGA
BsiEI 65 CGGCCG CG[GA][CT]^CG
BspEI 446 TCCGGA T^CCGGA
BspMI 14 GCAGGT ACCTGC(4/8)
BsrBI 119 GAGCGG CCGCTC(-3/-3)
BssSI 135 CTCGTG CACGAG(-5/-1)
DraI 588 TTTAAA TTT^AAA
DsaI 182 CCATGG C^C[GA][CT]GG
EaeI 65 CGGCCG [CT]^GGCC[GA]
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
HaeII 85 GGCGCC [GA]GCGC^[CT]
Hin1I 85 GGCGCC G[GA]^CG[CT]C
KasI 85 GGCGCC G^GCGCC
MaeI 325 CTAG C^TAG
MroI 446 TCCGGA T^CCGGA
NarI 85 GGCGCC GG^CGCC
NcoI 182 CCATGG C^CATGG
PflFI 439 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 765 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 339 CAGCTG CAG^CTG
RsaI 562 GTAC GT^AC
SacI 680 GAGCTC GAGCT^C
SfoI 85 GGCGCC GGC^GCC
TaqI 411 TCGA T^CGA
TfiI 6 GATTC G^A[AT]TC
Tsp45I 880 GTCAC ^GT[GC]AC
TspRI 306 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 439 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 411 TCGA T^CGA
Van91I 765 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 798 ACGTGT A^C[GA][CT]GT
846 ACACGT
AvaI 112 CCCGAG C^[CT]CG[GA]G
189 CTCGGG
BanI 85 GGCGCC G^G[CT][GA]CC
631 GGTGCC
BglI 58 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
203 GCCCTCCTGGC
BsaWI 290 TCCGGT [AT]^CCGG[AT]
446 TCCGGA
BsoBI 112 CCCGAG C^[CT]CG[GA]G
189 CTCGGG
CfoI 86 GCGC GCG^C
973 GCGC
DdeI 10 CTGAG C^T[ACGT]AG
734 CTCAG
DpnII 243 GATC ^GATC
703 GATC
EarI 94 GAAGAG CTCTTC(1/4)
541 GAAGAG
EcoNI 258 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
497 CCTGCAAAAGG
HhaI 86 GCGC GCG^C
973 GCGC
HinP1I 86 GCGC G^CGC
973 GCGC
HincII 655 GTTAAC GT[CT]^[GA]AC
966 GTTAAC
HindII 655 GTTAAC GT[CT]^[GA]AC
966 GTTAAC
HpaI 655 GTTAAC GTT^AAC
966 GTTAAC
Ksp632I 94 GAAGAG CTCTTC(1/4)
541 GAAGAG
MaeIII 880 GTCAC ^GT[ACGT]AC
1029 GTTAC
MboI 243 GATC ^GATC
703 GATC
MspA1I 154 CCGCTG C[AC]G^C[GT]G
339 CAGCTG
NdeII 243 GATC ^GATC
703 GATC
PstI 303 CTGCAG CTGCA^G
555 CTGCAG
Sau3AI 243 GATC ^GATC
703 GATC
SfaNI 751 GATGC GCATC(5/9)
991 GATGC
SmlI 262 CTCAAG C^T[CT][GA]AG
789 CTTGAG
SphI 478 GCATGC GCATG^C
575 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MboII MnlI MseI MspI
MvaI MwoI NciI NlaIII NlaIV
NspI PleI Sau96I ScrFI SfcI
StyI Tru9I TseI Tsp509I