Restriction map for
pFN21AB7606
(Circular) (Six-base) MAPSORT of: pFN21AB7606.seq Check: 3661 from: 1 to: 5773
pFN21AB7606 5773 bp
With 182 enzymes: SgfI *
March 28, 2012 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2010 2010
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3836 5220 5488 1632
Size: 1067 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 1067 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 2803 2851 4672 5649 1203
Size: 1600 48 1821 977 1327
Fragments arranged by size:
1821 1600 1327 977 48
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 2014 3516 5088 2014
Size: 1502 1572 2699
Fragments arranged by size:
2699 1572 1502
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2194 3098 1870
Size: 73 174 77 904 4545
Fragments arranged by size:
4545 904 174 77 73
AvrII C'CTAG_G
Cuts at: 2329 2329
Size: 5773
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2801 2834 2801
Size: 33 5740
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2636 3094 4549 618
Size: 325 217 627 303 546 458 1455 1842
Fragments arranged by size:
1842 1455 627 546 458 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2034 2117 2201 2689 3092 1391
Size: 643 83 84 488 403 4072
Fragments arranged by size:
4072 643 488 403 84 83
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 5773
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3964 3998 1903
Size: 34 50 34 1943 34 3678
Fragments arranged by size:
3678 1943 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 2214 4384
Size: 107 122 71 631 605 397 145 2170
Cuts at: 4384 136
Size: 1525
Fragments arranged by size:
2170 1525 631 605 397 145 122 107
71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2641 2955 3834 4990 1790
Size: 851 314 879 1156 2573
Fragments arranged by size:
2573 1156 879 851 314
BmgBI CAC'GTC
Cuts at: 1318 2804 2854 1318
Size: 1486 50 4237
Fragments arranged by size:
4237 1486 50
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2455 2876 3504
Size: 416 53 83 399 24 1038 421 628
Cuts at: 3504 4462 442
Size: 958 1753
Fragments arranged by size:
1753 1038 958 628 421 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2753 2887 3008 4433 1239
Size: 1025 489 134 121 1425 2579
Fragments arranged by size:
2579 1425 1025 489 134 121
Bpu10I CC'TnA_GC
Cuts at: 2739 2739
Size: 5773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2815 3898 4763 5061 2252
Size: 563 1083 865 298 2964
Fragments arranged by size:
2964 1083 865 563 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2950 3564 493
Size: 711 1746 614 2702
Fragments arranged by size:
2702 1746 711 614
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 3580 3962 4597
Size: 53 83 186 936 558 1489 382 635
Cuts at: 4597 275
Size: 1451
Fragments arranged by size:
1489 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2295 2451 4206 4604 4878 5025 5436 2295
Size: 156 1755 398 274 147 411 2632
Fragments arranged by size:
2632 1755 411 398 274 156 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2751 2781 2858
Size: 30 165 30 726 30 592 30 77
Cuts at: 2858 2888 1178
Size: 30 4063
Fragments arranged by size:
4063 726 592 165 77 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2137 2140 2543 2633 1186
Size: 871 80 3 403 90 4326
Fragments arranged by size:
4326 871 403 90 80 3
BseYI C'CCAG_C
Cuts at: 4976 5472 4976
Size: 496 5277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3223 3984 5012 1540
Size: 275 186 72 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 2147 2689 2839 3834 3919 4990 2147
Size: 542 150 995 85 1071 2930
Fragments arranged by size:
2930 1071 995 542 150 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2117 2147 2201 2641 2689 2839
Size: 399 244 83 30 54 440 48 150
Cuts at: 2839 2955 3092 3834 3919 4990 1391
Size: 116 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 244 150 137
116 85 83 54 48 30
BspEI T'CCGG_A
Cuts at: 2451 5436 2451
Size: 2985 2788
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2010 3128 844
Size: 1166 1118 3489
Fragments arranged by size:
3489 1166 1118
BsrBI CCG'CTC
Cuts at: 1394 1823 2126 3660 1394
Size: 429 303 1534 3507
Fragments arranged by size:
3507 1534 429 303
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 2140 3833 4845 2140
Size: 1693 1012 3068
Fragments arranged by size:
3068 1693 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 2316 2489 2735 794
Size: 1441 81 173 246 3832
Fragments arranged by size:
3832 1441 246 173 81
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2187 513
Size: 552 1122 4099
Fragments arranged by size:
4099 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 3289 4083 4103 2317
Size: 972 794 20 3987
Fragments arranged by size:
3987 972 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 2595 3077 2595
Size: 482 5291
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 3220 4109
Size: 54 1425 50 275 18 240 1150 889
Cuts at: 4109 5738 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1150 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 3220 1537
Size: 275 258 1150 4090
Fragments arranged by size:
4090 1150 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2541 3703 1353
Size: 741 447 1162 3423
Fragments arranged by size:
3423 1162 741 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2068 4360 4734 4880 387
Size: 1064 138 479 2292 374 146 1280
Fragments arranged by size:
2292 1280 1064 479 374 146 138
EcoICRI GAG'CTC
Cuts at: 2687 2687
Size: 5773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2699 2753 2887 3008 3836 4433
Size: 393 632 435 54 134 121 828 597
Cuts at: 4433 5220 5488 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 632 597 435 393 268
134 121 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2506 2267
Size: 239 5534
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2094 4920 1678
Size: 416 2826 2531
Fragments arranged by size:
2826 2531 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2123 2129 2155
Size: 32 135 32 436 32 83 6 26
Cuts at: 2155 2161 4414 4446 4488 4520 1373
Size: 6 2253 32 42 32 2626
Fragments arranged by size:
2626 2253 436 135 83 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 2973 3117 3363 669
Size: 988 281 724 311 144 246 3079
Fragments arranged by size:
3079 988 724 311 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 2662 2973 3363 2662
Size: 311 390 5072
Fragments arranged by size:
5072 390 311
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4887 5071 1305
Size: 3582 184 2007
Fragments arranged by size:
3582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 4090 4249 518
Size: 648 2565 359 159 2042
Fragments arranged by size:
2565 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2346 3189 3866 5014 5259 1606
Size: 555 185 843 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 555 245 185
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 5773
NcoI C'CATG_G
Cuts at: 513 1065 2187 513
Size: 552 1122 4099
Fragments arranged by size:
4099 1122 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2294 2487 2584 3131 4676 1132
Size: 97 1065 193 97 547 1545 2229
Fragments arranged by size:
2229 1545 1065 547 193 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4105
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PstI C_TGCA'G
Cuts at: 830 2312 2564 3125 830
Size: 1482 252 561 3478
Fragments arranged by size:
3478 1482 561 252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2346 2346
Size: 5773
SacI G_AGCT'C
Cuts at: 2689 2689
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2058 2308 2560 3121 4256 4937 5128
Size: 219 1013 250 252 561 1135 681 191
Cuts at: 5128 826
Size: 1471
Fragments arranged by size:
1471 1135 1013 681 561 252 250 219
191
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 5773
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 2794 3877 4778 5040 820
Size: 197 926 324 527 1083 901 262 1553
Fragments arranged by size:
1553 1083 926 901 527 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 2487 2584 3131 1132
Size: 1355 97 547 3774
Fragments arranged by size:
3774 1355 547 97
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2187 2329 2460 3212 513
Size: 552 1122 142 131 752 3074
Fragments arranged by size:
3074 1122 752 552 142 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2932 3800 3817 3970 4155 4600 325
Size: 1255 1352 868 17 153 185 445 1498
Fragments arranged by size:
1498 1352 1255 868 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2566 4019 5731
Size: 274 80 33 51 1443 589 1453 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1712 1453 1443 589 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 2858 2890 5733 5765 2751
Size: 32 75 32 2843 32 2759
Fragments arranged by size:
2843 2759 75 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2447 1316
Size: 1131 4642
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI