(Linear) (Six-base) MAPSORT of: KIBB7606.seq Check: 9647 from: 1 to: 1068 KIBB7606.orf With 182 enzymes: SgfI * March 22, 2012 15:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 5 1068 Size: 5 1063 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1068 Size: 694 374 AflIII A'CryG_T Cuts at: 0 798 846 1068 Size: 798 48 222 Fragments arranged by size: 798 222 48 AlwNI CAG_nnn'CTG Cuts at: 0 9 1068 Size: 9 1059 AvaI C'yCGr_G Cuts at: 0 112 189 1068 Size: 112 77 879 Fragments arranged by size: 879 112 77 AvrII C'CTAG_G Cuts at: 0 324 1068 Size: 324 744 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 796 829 1068 Size: 796 33 239 Fragments arranged by size: 796 239 33 BanI G'GyrC_C Cuts at: 0 85 631 1068 Size: 85 546 437 Fragments arranged by size: 546 437 85 BanII G_rGCy'C Cuts at: 0 29 112 196 684 1068 Size: 29 83 84 488 384 Fragments arranged by size: 488 384 84 83 29 BbeI G_GCGC'C Cuts at: 0 89 1068 Size: 89 979 BglI GCCn_nnn'nGGC Cuts at: 0 64 209 1068 Size: 64 145 859 Fragments arranged by size: 859 145 64 Bme1580I G_kGCm'C Cuts at: 0 636 950 1068 Size: 636 314 118 Fragments arranged by size: 636 314 118 BmgBI CAC'GTC Cuts at: 0 799 849 1068 Size: 799 50 219 Fragments arranged by size: 799 219 50 BmrI ACTGGGnnnn_n' Cuts at: 0 450 871 1068 Size: 450 421 197 Fragments arranged by size: 450 421 197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 748 882 1003 1068 Size: 259 489 134 121 65 Fragments arranged by size: 489 259 134 121 65 Bpu10I CC'TnA_GC Cuts at: 0 734 1068 Size: 734 334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 810 1068 Size: 247 563 258 Fragments arranged by size: 563 258 247 BsaAI yAC'GTr Cuts at: 0 945 1068 Size: 945 123 BsaHI Gr'CG_yC Cuts at: 0 86 1068 Size: 86 982 BsaWI w'CCGG_w Cuts at: 0 290 446 1068 Size: 290 156 622 Fragments arranged by size: 622 290 156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 124 154 746 776 853 883 1068 Size: 124 30 592 30 77 30 185 Fragments arranged by size: 592 185 124 77 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 132 135 538 628 1068 Size: 52 80 3 403 90 440 Fragments arranged by size: 440 403 90 80 52 3 BsiEI CG_ry'CG Cuts at: 0 68 1068 Size: 68 1000 BsiHKAI G_wGCw'C Cuts at: 0 142 684 834 1068 Size: 142 542 150 234 Fragments arranged by size: 542 234 150 142 Bsp1286I G_dGCh'C Cuts at: 0 29 112 142 196 636 684 834 950 Size: 29 83 30 54 440 48 150 116 Cuts at: 950 1068 Size: 118 Fragments arranged by size: 440 150 118 116 83 54 48 30 29 BspEI T'CCGG_A Cuts at: 0 446 1068 Size: 446 622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 5 1068 Size: 5 1063 BsrBI CCG'CTC Cuts at: 0 121 1068 Size: 121 947 BssSI C'ACGA_G Cuts at: 0 135 1068 Size: 135 933 BstAPI GCAn_nnn'nTGC Cuts at: 0 230 311 484 730 1068 Size: 230 81 173 246 338 Fragments arranged by size: 338 246 230 173 81 BtgI C'CryG_G Cuts at: 0 182 1068 Size: 182 886 BtsI GCAGTG_nn' Cuts at: 0 312 1068 Size: 312 756 DraI TTT'AAA Cuts at: 0 590 1068 Size: 590 478 EaeI y'GGCC_r Cuts at: 0 65 1068 Size: 65 1003 EagI C'GGCC_G Cuts at: 0 65 1068 Size: 65 1003 EarI CTCTTCn'nnn_ Cuts at: 0 89 536 1068 Size: 89 447 532 Fragments arranged by size: 532 447 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 63 1068 Size: 63 1005 EcoICRI GAG'CTC Cuts at: 0 682 1068 Size: 682 386 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 694 748 882 1003 1068 Size: 259 435 54 134 121 65 Fragments arranged by size: 435 259 134 121 65 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 262 501 1068 Size: 262 239 567 Fragments arranged by size: 567 262 239 HaeII r_GCGC'y Cuts at: 0 89 1068 Size: 89 979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 3 35 118 124 150 156 1068 Size: 3 32 83 6 26 6 912 Fragments arranged by size: 912 83 32 26 6 6 3 HincII GTy'rAC Cuts at: 0 657 968 1068 Size: 657 311 100 Fragments arranged by size: 657 311 100 HpaI GTT'AAC Cuts at: 0 657 968 1068 Size: 657 311 100 Fragments arranged by size: 657 311 100 KasI G'GCGC_C Cuts at: 0 85 1068 Size: 85 983 MspA1I CmG'CkG Cuts at: 0 156 341 1068 Size: 156 185 727 Fragments arranged by size: 727 185 156 NarI GG'CG_CC Cuts at: 0 86 1068 Size: 86 982 NcoI C'CATG_G Cuts at: 0 182 1068 Size: 182 886 NspI r_CATG'y Cuts at: 0 289 482 579 1068 Size: 289 193 97 489 Fragments arranged by size: 489 289 193 97 PflMI CCAn_nnn'nTGG Cuts at: 0 771 1068 Size: 771 297 PstI C_TGCA'G Cuts at: 0 307 559 1068 Size: 307 252 509 Fragments arranged by size: 509 307 252 PvuII CAG'CTG Cuts at: 0 341 1068 Size: 341 727 SacI G_AGCT'C Cuts at: 0 684 1068 Size: 684 384 SfcI C'TryA_G Cuts at: 0 53 303 555 1068 Size: 53 250 252 513 Fragments arranged by size: 513 252 250 53 SfoI GGC'GCC Cuts at: 0 87 1068 Size: 87 981 SmlI C'TyrA_G Cuts at: 0 262 789 1068 Size: 262 527 279 Fragments arranged by size: 527 279 262 SphI G_CATG'C Cuts at: 0 482 579 1068 Size: 482 97 489 Fragments arranged by size: 489 482 97 StyI C'CwwG_G Cuts at: 0 182 324 455 1068 Size: 182 142 131 613 Fragments arranged by size: 613 182 142 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 927 1068 Size: 927 141 TatI w'GTAC_w Cuts at: 0 561 1068 Size: 561 507 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 746 778 853 885 1068 Size: 746 32 75 32 183 Fragments arranged by size: 746 183 75 32 32 Tth111I GACn'n_nGTC Cuts at: 0 442 1068 Size: 442 626 Enzymes that do cut: AarI AcuI AflIII AlwNI AvaI AvrII BaeI BanI BanII BbeI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BssSI BstAPI BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI HaeII Hin4I HincII HpaI KasI MspA1I NarI NcoI NspI PflMI PstI PvuII SacI SfcI SfoI SmlI SphI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI BsaBI BseYI BsgI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI MfeI MluI MmeI MscI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI