Restriction map for
pFN21AB9638
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BstBI BstEII BstPI BstYI
Bsu36I ClaI CpoI DraI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MboII MfeI
MflI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1231 GTATAC GT^[AC][GT]AC
Alw44I 349 GTGCAC G^TGCAC
ApaLI 349 GTGCAC G^TGCAC
BbeI 625 GGCGCC GGCGC^C
BcgI 1028 GCACGTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 900 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 900 GCTGAGC GC^T[ACGT]AGC
BsaI 955 GAGACC GGTCTC(1/5)
BsmAI 955 GAGAC GTCTC(1/5)
BspLU11I 914 ACATGT A^CATGT
BsrBI 463 CCGCTC CCGCTC(-3/-3)
BsrI 213 CCAGT ACTGG(1/-1)
Bst1107I 1231 GTATAC GTA^TAC
BstAPI 15 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1231 GTATAC GTA^TAC
CelII 900 GCTGAGC GC^T[ACGT]AGC
DraIII 190 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 957 GACCGACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1220 CCATGG C^C[AT][AT]GG
HinfI 961 GACTC G^A[ACGT]TC
HphI 1106 TCACC GGTGA(8/7)
KasI 625 GGCGCC G^GCGCC
MaeIII 854 GTAAC ^GT[ACGT]AC
NarI 625 GGCGCC GG^CGCC
NcoI 1220 CCATGG C^CATGG
NotI 103 GCGGCCGC GC^GGCCGC
PaeR7I 1060 CTCGAG C^TCGAG
PflMI 189 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 961 GACTC GAGTC(4/5)
SfoI 625 GGCGCC GGC^GCC
SgrAI 1301 CGCCGGCG C[GA]^CCGG[CT]G
SphI 120 GCATGC GCATG^C
StyI 1220 CCATGG C^C[AT][AT]GG
Van91I 189 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1060 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 76 GACGCC G[GA]^CG[CT]C
625 GGCGCC
AflIII 857 ACGTGT A^C[GA][CT]GT
914 ACATGT
AlwNI 16 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
148 CAGAAGCTG
ApaI 30 GGGCCC GGGCC^C
411 GGGCCC
AspHI 349 GTGCAC G[AT]GC[AT]^C
727 GAGCAC
BalI 2 TGGCCA TGG^CCA
582 TGGCCA
BanII 30 GGGCCC G[GA]GC[CT]^C
411 GGGCCC
BsaHI 76 GACGCC G[GA]^CG[CT]C
625 GGCGCC
BseRI 230 GAGGAG GAGGAG(10/8)
952 GAGGAG
BsiHKAI 349 GTGCAC G[AT]GC[AT]^C
727 GAGCAC
BspMI 20 ACCTGC ACCTGC(4/8)
686 ACCTGC
BssHII 340 GCGCGC G^CGCGC
1055 GCGCGC
BstXI 980 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1034 CCATCGAGGTGG
DdeI 901 CTGAG C^T[ACGT]AG
1344 CTCAG
DpnII 996 GATC ^GATC
1104 GATC
HgaI 76 GACGC GACGC(5/10)
1151 GCGTC
Hin1I 76 GACGCC G[GA]^CG[CT]C
625 GGCGCC
MboI 996 GATC ^GATC
1104 GATC
MluNI 2 TGGCCA TGG^CCA
582 TGGCCA
MscI 2 TGGCCA TGG^CCA
582 TGGCCA
NdeII 996 GATC ^GATC
1104 GATC
PstI 895 CTGCAG CTGCA^G
1126 CTGCAG
Sau3AI 996 GATC ^GATC
1104 GATC
SfcI 895 CTGCAG C^T[GA][CT]AG
1126 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsgI BsiEI
BsiYI BslI BsoBI Bsp1286I BsrFI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI KspI MaeI MaeII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV NspI PvuII RsaI
SacII Sau96I ScrFI SfaNI SmaI
SmlI TaqI TseI TspRI TthHB8I
XmaCI XmaI