(Circular) (Six-base) MAPSORT of: pFN21AB9638.seq Check: 198 from: 1 to: 6058 pFN21AB9638 6058 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2700 2700 Size: 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3237 3401 1937 Size: 1300 164 4594 Fragments arranged by size: 4594 1300 164 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4121 5505 5773 1632 Size: 2489 1384 268 1917 Fragments arranged by size: 2489 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 2862 2919 4957 5934 1203 Size: 1659 57 2038 977 1327 Fragments arranged by size: 2038 1659 1327 977 57 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2026 2158 3801 5373 2026 Size: 132 1643 1572 2711 Fragments arranged by size: 2711 1643 1572 132 ApaI G_GGCC'C Cuts at: 2039 2420 2039 Size: 381 5677 ApaLI G'TGCA_C Cuts at: 2354 4115 5271 2354 Size: 1761 1156 3141 Fragments arranged by size: 3141 1761 1156 ApoI r'AATT_y Cuts at: 1493 3367 3593 3745 5854 1493 Size: 1874 226 152 2109 1697 Fragments arranged by size: 2109 1874 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2438 2471 2497 2698 3065 3383 1870 Size: 73 495 33 26 201 367 318 4545 Fragments arranged by size: 4545 495 367 318 201 73 33 26 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2300 2630 3379 4834 Size: 325 217 627 270 243 330 749 1455 Cuts at: 4834 618 Size: 1842 Fragments arranged by size: 1842 1455 749 627 330 325 270 243 217 BanII G_rGCy'C Cuts at: 1391 2039 2420 3377 1391 Size: 648 381 957 4072 Fragments arranged by size: 4072 957 648 381 BbeI G_GCGC'C Cuts at: 2634 2634 Size: 6058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5087 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3022 3056 4249 4283 1903 Size: 34 1085 34 1193 34 3678 Fragments arranged by size: 3678 1193 1085 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 2090 2473 Size: 107 122 71 631 605 371 47 383 Cuts at: 2473 4669 136 Size: 2196 1525 Fragments arranged by size: 2196 1525 631 605 383 371 122 107 71 47 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 2906 3475 2906 Size: 569 5489 Bme1580I G_kGCm'C Cuts at: 1790 2039 2060 2358 2420 2849 3089 4119 5275 Size: 249 21 298 62 429 240 1030 1156 Cuts at: 5275 1790 Size: 2573 Fragments arranged by size: 2573 1156 1030 429 298 249 240 62 21 BmgBI CAC'GTC Cuts at: 1318 2830 3036 1318 Size: 1512 206 4340 Fragments arranged by size: 4340 1512 206 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 3789 4747 Size: 416 53 83 399 24 795 1577 958 Cuts at: 4747 442 Size: 1753 Fragments arranged by size: 1753 1577 958 795 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2983 2983 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 2789 2975 3158 4718 1239 Size: 1385 165 186 183 1560 2579 Fragments arranged by size: 2579 1560 1385 186 183 165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 3065 4183 5048 5346 2915 Size: 150 1118 865 298 3627 Fragments arranged by size: 3627 1118 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2954 4721 882 Size: 617 1455 1767 2219 Fragments arranged by size: 2219 1767 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2631 3865 4247 Size: 53 83 186 936 549 549 1234 382 Cuts at: 4247 4882 275 Size: 635 1451 Fragments arranged by size: 1451 1234 936 635 549 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2250 2972 1186 Size: 1064 722 4272 Fragments arranged by size: 4272 1064 722 BseYI C'CCAG_C Cuts at: 2176 2463 2753 2809 2842 5261 5757 2176 Size: 287 290 56 33 2419 496 2477 Fragments arranged by size: 2477 2419 496 290 287 56 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 2549 2561 2657 2678 3302 3374 Size: 573 435 378 12 96 21 624 72 Cuts at: 3374 1163 Size: 3847 Fragments arranged by size: 3847 624 573 435 378 96 72 21 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2308 3207 3508 4269 5297 Size: 275 186 111 196 899 301 761 1028 Cuts at: 5297 1540 Size: 2301 Fragments arranged by size: 2301 1028 899 761 301 275 196 186 111 BsiHKAI G_wGCw'C Cuts at: 2358 2736 4119 4204 5275 2358 Size: 378 1383 85 1071 3141 Fragments arranged by size: 3141 1383 1071 378 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2060 2358 2420 2736 2849 3089 Size: 399 249 21 298 62 316 113 240 Cuts at: 3089 3377 4119 4204 5275 1391 Size: 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 298 288 249 240 113 85 62 21 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 2700 3346 3413 844 Size: 1190 666 646 67 3489 Fragments arranged by size: 3489 1190 666 646 67 BsrBI CCG'CTC Cuts at: 1394 1823 2470 3945 1394 Size: 429 647 1475 3507 Fragments arranged by size: 3507 1475 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 4727 65 Size: 1183 3305 174 1396 Fragments arranged by size: 3305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2258 2303 3113 3171 3206 3307 4702 Size: 110 448 45 810 58 35 101 1395 Cuts at: 4702 4889 1700 Size: 187 2869 Fragments arranged by size: 2869 1395 810 448 187 110 101 58 45 35 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 2345 3060 1925 Size: 420 715 4923 Fragments arranged by size: 4923 715 420 BssSI C'ACGA_G Cuts at: 2168 2579 2591 3104 4118 5130 2168 Size: 411 12 513 1014 1012 3096 Fragments arranged by size: 3096 1014 1012 513 411 12 BstAPI GCAn_nnn'nTGC Cuts at: 794 2026 794 Size: 1232 4826 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2992 3046 2992 Size: 54 6004 BstYI r'GATC_y Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053 Size: 1416 367 385 17 1452 86 12 346 Cuts at: 6053 1972 Size: 1977 Fragments arranged by size: 1977 1452 1416 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3238 3238 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 2085 2112 2199 2229 2635 Size: 552 939 26 55 27 87 30 406 Cuts at: 2635 3225 513 Size: 590 3346 Fragments arranged by size: 3346 939 590 552 406 87 55 30 27 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3261 487 Size: 683 56 2035 3284 Fragments arranged by size: 3284 2035 683 56 BtsI GCAGTG_nn' Cuts at: 3338 3574 4368 4388 3338 Size: 236 794 20 5008 Fragments arranged by size: 5008 794 236 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DraIII CAC_nnn'GTG Cuts at: 2200 2200 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2968 5065 809 Size: 507 1652 2097 1802 Fragments arranged by size: 2097 1802 1652 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2109 2305 Size: 54 1425 50 275 18 177 102 196 Cuts at: 2305 2421 2520 2587 3204 3505 4394 6023 8 Size: 116 99 67 617 301 889 1629 43 Fragments arranged by size: 1629 1425 889 617 301 275 196 177 116 102 99 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 2305 3204 3505 1537 Size: 275 297 196 899 301 4090 Fragments arranged by size: 4090 899 301 297 275 196 EarI CTCTTCn'nnn_ Cuts at: 1353 3988 1353 Size: 2635 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2061 2346 4645 5019 5165 387 Size: 1064 138 472 285 2299 374 146 1280 Fragments arranged by size: 2299 1280 1064 472 374 285 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 2789 2975 3158 4121 4718 5505 Size: 393 992 165 186 183 963 597 787 Cuts at: 5505 5773 1239 Size: 268 1524 Fragments arranged by size: 1524 992 963 787 597 393 268 186 183 165 EcoO109I rG'GnC_Cy Cuts at: 2035 2036 2896 3257 2035 Size: 1 860 361 4836 Fragments arranged by size: 4836 860 361 1 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2634 2843 3317 5205 1678 Size: 956 209 474 1888 2531 Fragments arranged by size: 2531 1888 956 474 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373 Size: 32 135 32 3127 32 42 32 2626 Fragments arranged by size: 3127 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KasI G'GCGC_C Cuts at: 2630 2630 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5172 5356 1305 Size: 3867 184 2007 Fragments arranged by size: 3867 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2589 10 Size: 54 1425 343 177 580 3479 Fragments arranged by size: 3479 1425 580 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 2652 3044 4016 4375 4534 518 Size: 648 1417 69 392 972 359 159 2042 Fragments arranged by size: 2042 1417 972 648 392 359 159 69 MspA1I CmG'CkG Cuts at: 1606 2032 2087 2114 2448 2602 2797 2905 2938 Size: 426 55 27 334 154 195 108 33 Cuts at: 2938 2976 3136 3474 4151 5299 5544 1606 Size: 38 160 338 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 426 338 334 245 195 160 154 108 55 38 33 27 NaeI GCC'GGC Cuts at: 2260 3208 3309 2260 Size: 948 101 5009 Fragments arranged by size: 5009 948 101 NarI GG'CG_CC Cuts at: 2631 2631 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 2004 3225 513 Size: 552 939 1221 3346 Fragments arranged by size: 3346 1221 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NgoMIV G'CCGG_C Cuts at: 2258 3206 3307 2258 Size: 948 101 5009 Fragments arranged by size: 5009 948 101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 2109 3505 2109 Size: 1396 4662 NspI r_CATG'y Cuts at: 1132 1229 2129 2657 2923 3416 4961 1132 Size: 97 900 528 266 493 1545 2229 Fragments arranged by size: 2229 1545 900 528 493 266 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 2919 4957 2919 Size: 2038 4020 PflMI CCAn_nnn'nTGG Cuts at: 1108 2200 1108 Size: 1092 4966 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 2035 2416 2035 Size: 381 5677 PstI C_TGCA'G Cuts at: 830 2904 3135 3410 830 Size: 2074 231 275 3478 Fragments arranged by size: 3478 2074 275 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2602 2905 3136 2602 Size: 303 231 5524 Fragments arranged by size: 5524 303 231 SacII CC_GC'GG Cuts at: 2033 2088 2115 2033 Size: 55 27 5976 Fragments arranged by size: 5976 55 27 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2900 3131 3406 4541 5222 5413 826 Size: 219 1855 231 275 1135 681 191 1471 Fragments arranged by size: 1855 1471 1135 681 275 231 219 191 SfoI GGC'GCC Cuts at: 2632 2632 Size: 6058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 3307 1700 Size: 1607 4451 SmaI CCC'GGG Cuts at: 1872 2440 2499 2700 3385 1872 Size: 568 59 201 685 4545 Fragments arranged by size: 4545 685 568 201 59 SmlI C'TyrA_G Cuts at: 820 1017 1943 2930 3065 3080 4162 5063 5325 Size: 197 926 987 135 15 1082 901 262 Cuts at: 5325 820 Size: 1553 Fragments arranged by size: 1553 1082 987 926 901 262 197 135 15 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 2129 3416 1132 Size: 997 1287 3774 Fragments arranged by size: 3774 1287 997 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2004 3225 3497 513 Size: 552 939 1221 272 3074 Fragments arranged by size: 3074 1221 939 552 272 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2978 4085 4102 4255 4440 4885 325 Size: 1255 1398 1107 17 153 185 445 1498 Fragments arranged by size: 1498 1398 1255 1107 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4304 6016 96 Size: 274 80 33 51 1443 2327 1712 138 Fragments arranged by size: 2327 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6018 6050 6018 Size: 32 6026 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 3065 1943 Size: 1122 4936 XmaI C'CCGG_G Cuts at: 1870 2438 2497 2698 3383 1870 Size: 568 59 201 685 4545 Fragments arranged by size: 4545 685 568 201 59 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI