(Linear) (Six-base) MAPSORT of: KIBB9638.seq Check: 9700 from: 1 to: 1353 KIBB9638.orf With 182 enzymes: SgfI * July 3, 2012 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 695 1353 Size: 695 658 AccI GT'mk_AC Cuts at: 0 1232 1353 Size: 1232 121 AflIII A'CryG_T Cuts at: 0 857 914 1353 Size: 857 57 439 Fragments arranged by size: 857 439 57 AlwNI CAG_nnn'CTG Cuts at: 0 21 153 1353 Size: 21 132 1200 Fragments arranged by size: 1200 132 21 ApaI G_GGCC'C Cuts at: 0 34 415 1353 Size: 34 381 938 Fragments arranged by size: 938 381 34 ApaLI G'TGCA_C Cuts at: 0 349 1353 Size: 349 1004 AvaI C'yCGr_G Cuts at: 0 433 466 492 693 1060 1353 Size: 433 33 26 201 367 293 Fragments arranged by size: 433 367 293 201 33 26 BanI G'GyrC_C Cuts at: 0 52 295 625 1353 Size: 52 243 330 728 Fragments arranged by size: 728 330 243 52 BanII G_rGCy'C Cuts at: 0 34 415 1353 Size: 34 381 938 Fragments arranged by size: 938 381 34 BbeI G_GCGC'C Cuts at: 0 629 1353 Size: 629 724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1017 1051 1353 Size: 1017 34 302 Fragments arranged by size: 1017 302 34 BglI GCCn_nnn'nGGC Cuts at: 0 38 85 468 1353 Size: 38 47 383 885 Fragments arranged by size: 885 383 47 38 BlpI GC'TnA_GC Cuts at: 0 901 1353 Size: 901 452 Bme1580I G_kGCm'C Cuts at: 0 34 55 353 415 844 1084 1353 Size: 34 21 298 62 429 240 269 Fragments arranged by size: 429 298 269 240 62 34 21 BmgBI CAC'GTC Cuts at: 0 825 1031 1353 Size: 825 206 322 Fragments arranged by size: 825 322 206 BmrI ACTGGGnnnn_n' Cuts at: 0 207 1353 Size: 207 1146 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 978 1353 Size: 978 375 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 784 970 1153 1353 Size: 619 165 186 183 200 Fragments arranged by size: 619 200 186 183 165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1060 1353 Size: 910 150 293 Fragments arranged by size: 910 293 150 BsaI GGTCTCn'nnnn_ Cuts at: 0 949 1353 Size: 949 404 BsaHI Gr'CG_yC Cuts at: 0 77 626 1353 Size: 77 549 727 Fragments arranged by size: 727 549 77 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 245 967 1353 Size: 245 722 386 Fragments arranged by size: 722 386 245 BseYI C'CCAG_C Cuts at: 0 171 458 748 804 837 1353 Size: 171 287 290 56 33 516 Fragments arranged by size: 516 290 287 171 56 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 544 556 652 673 1297 1353 Size: 166 378 12 96 21 624 56 Fragments arranged by size: 624 378 166 96 56 21 12 BsiEI CG_ry'CG Cuts at: 0 107 303 1202 1353 Size: 107 196 899 151 Fragments arranged by size: 899 196 151 107 BsiHKAI G_wGCw'C Cuts at: 0 353 731 1353 Size: 353 378 622 Fragments arranged by size: 622 378 353 Bsp1286I G_dGCh'C Cuts at: 0 34 55 353 415 731 844 1084 1353 Size: 34 21 298 62 316 113 240 269 Fragments arranged by size: 316 298 269 240 113 62 34 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 29 695 1353 Size: 29 666 658 Fragments arranged by size: 666 658 29 BsrBI CCG'CTC Cuts at: 0 465 1353 Size: 465 888 BsrFI r'CCGG_y Cuts at: 0 253 298 1108 1166 1201 1302 1353 Size: 253 45 810 58 35 101 51 Fragments arranged by size: 810 253 101 58 51 45 35 BssHII G'CGCG_C Cuts at: 0 340 1055 1353 Size: 340 715 298 Fragments arranged by size: 715 340 298 BssSI C'ACGA_G Cuts at: 0 163 574 586 1099 1353 Size: 163 411 12 513 254 Fragments arranged by size: 513 411 254 163 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 21 1353 Size: 21 1332 BstXI CCAn_nnnn'nTGG Cuts at: 0 987 1041 1353 Size: 987 54 312 Fragments arranged by size: 987 312 54 BstZ17I GTA'TAC Cuts at: 0 1233 1353 Size: 1233 120 BtgI C'CryG_G Cuts at: 0 25 80 107 194 224 630 1220 1353 Size: 25 55 27 87 30 406 590 133 Fragments arranged by size: 590 406 133 87 55 30 27 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1256 1353 Size: 1256 97 BtsI GCAGTG_nn' Cuts at: 0 1333 1353 Size: 1333 20 DraIII CAC_nnn'GTG Cuts at: 0 195 1353 Size: 195 1158 DrdI GACnn_nn'nnGTC Cuts at: 0 963 1353 Size: 963 390 EaeI y'GGCC_r Cuts at: 0 2 104 300 416 515 582 1199 1353 Size: 2 102 196 116 99 67 617 154 Fragments arranged by size: 617 196 154 116 102 99 67 2 EagI C'GGCC_G Cuts at: 0 104 300 1199 1353 Size: 104 196 899 154 Fragments arranged by size: 899 196 154 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 56 341 1353 Size: 56 285 1012 Fragments arranged by size: 1012 285 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 784 970 1153 1353 Size: 619 165 186 183 200 Fragments arranged by size: 619 200 186 183 165 EcoO109I rG'GnC_Cy Cuts at: 0 30 31 891 1252 1353 Size: 30 1 860 361 101 Fragments arranged by size: 860 361 101 30 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 825 1353 Size: 825 528 HaeII r_GCGC'y Cuts at: 0 629 838 1312 1353 Size: 629 209 474 41 Fragments arranged by size: 629 474 209 41 KasI G'GCGC_C Cuts at: 0 625 1353 Size: 625 728 MscI TGG'CCA Cuts at: 0 4 584 1353 Size: 4 580 769 Fragments arranged by size: 769 580 4 MslI CAynn'nnrTG Cuts at: 0 578 647 1039 1353 Size: 578 69 392 314 Fragments arranged by size: 578 392 314 69 MspA1I CmG'CkG Cuts at: 0 27 82 109 443 597 792 900 933 Size: 27 55 27 334 154 195 108 33 Cuts at: 933 971 1131 1353 Size: 38 160 222 Fragments arranged by size: 334 222 195 160 154 108 55 38 33 27 27 NaeI GCC'GGC Cuts at: 0 255 1203 1304 1353 Size: 255 948 101 49 Fragments arranged by size: 948 255 101 49 NarI GG'CG_CC Cuts at: 0 626 1353 Size: 626 727 NcoI C'CATG_G Cuts at: 0 1220 1353 Size: 1220 133 NgoMIV G'CCGG_C Cuts at: 0 253 1201 1302 1353 Size: 253 948 101 51 Fragments arranged by size: 948 253 101 51 NotI GC'GGCC_GC Cuts at: 0 104 1353 Size: 104 1249 NspI r_CATG'y Cuts at: 0 124 652 918 1353 Size: 124 528 266 435 Fragments arranged by size: 528 435 266 124 PciI A'CATG_T Cuts at: 0 914 1353 Size: 914 439 PflMI CCAn_nnn'nTGG Cuts at: 0 195 1353 Size: 195 1158 PspOMI G'GGCC_C Cuts at: 0 30 411 1353 Size: 30 381 942 Fragments arranged by size: 942 381 30 PstI C_TGCA'G Cuts at: 0 899 1130 1353 Size: 899 231 223 Fragments arranged by size: 899 231 223 PvuII CAG'CTG Cuts at: 0 597 900 1131 1353 Size: 597 303 231 222 Fragments arranged by size: 597 303 231 222 SacII CC_GC'GG Cuts at: 0 28 83 110 1353 Size: 28 55 27 1243 Fragments arranged by size: 1243 55 28 27 SfcI C'TryA_G Cuts at: 0 895 1126 1353 Size: 895 231 227 Fragments arranged by size: 895 231 227 SfoI GGC'GCC Cuts at: 0 627 1353 Size: 627 726 SgrAI Cr'CCGG_yG Cuts at: 0 1302 1353 Size: 1302 51 SmaI CCC'GGG Cuts at: 0 435 494 695 1353 Size: 435 59 201 658 Fragments arranged by size: 658 435 201 59 SmlI C'TyrA_G Cuts at: 0 925 1060 1075 1353 Size: 925 135 15 278 Fragments arranged by size: 925 278 135 15 SphI G_CATG'C Cuts at: 0 124 1353 Size: 124 1229 StyI C'CwwG_G Cuts at: 0 1220 1353 Size: 1220 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 973 1353 Size: 973 380 XhoI C'TCGA_G Cuts at: 0 1060 1353 Size: 1060 293 XmaI C'CCGG_G Cuts at: 0 433 492 693 1353 Size: 433 59 201 660 Fragments arranged by size: 660 433 201 59 Enzymes that do cut: AarI AccI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstZ17I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EciI Eco57MI EcoO109I FalI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI PspOMI PstI PvuII SacII SfcI SfoI SgrAI SmaI SmlI SphI StyI TaqII XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstYI Bsu36I ClaI CspCI DraI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XmnI ZraI