Restriction map for
pFN21AE3895
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
TspRI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 82 GTCTAC GT^[AC][GT]AC
AluI 215 AGCT AG^CT
AspHI 214 GAGCTC G[AT]GC[AT]^C
AvaI 75 CTCGGG C^[CT]CG[GA]G
BanI 9 GGTGCC G^G[CT][GA]CC
BclI 146 TGATCA T^GATCA
BglII 107 AGATCT A^GATCT
BsaAI 325 TACGTA [CT]AC^GT[GA]
BsiHKAI 214 GAGCTC G[AT]GC[AT]^C
BsmFI 140 GGGAC GGGAC(10/14)
BsoBI 75 CTCGGG C^[CT]CG[GA]G
BsrFI 6 GCCGGT [GA]^CCGG[CT]
BsrI 321 CCAGT ACTGG(1/-1)
BssHII 30 GCGCGC G^CGCGC
BstEII 153 GGTCACC G^GT[ACGT]ACC
BstF5I 313 GGATG GGATG(2/0)
BstPI 153 GGTCACC G^GT[ACGT]ACC
BstYI 107 AGATCT [GA]^GATC[CT]
Cfr10I 6 GCCGGT [GA]^CCGG[CT]
DdeI 247 CTTAG C^T[ACGT]AG
DraII 140 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 140 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 153 GGTCACC G^GT[ACGT]ACC
FbaI 146 TGATCA T^GATCA
FokI 313 GGATG GGATG(9/13)
HapII 7 CCGG C^CGG
HinfI 337 GATTC G^A[ACGT]TC
HpaII 7 CCGG C^CGG
HphI 155 TCACC GGTGA(8/7)
MaeII 326 ACGT A^CGT
MaeIII 154 GTCAC ^GT[ACGT]AC
MflI 107 AGATCT [GA]^GATC[CT]
MspA1I 13 CCGCTG C[AC]G^C[GT]G
MspI 7 CCGG C^CGG
NcoI 209 CCATGG C^CATGG
NlaIII 210 CATG CATG^
PpuMI 140 GGGACCT [GA]G^G[AT]CC[CT]
PstI 16 CTGCAG CTGCA^G
SacI 214 GAGCTC GAGCT^C
SfcI 16 CTGCAG C^T[GA][CT]AG
SmlI 240 CTCAAG C^T[CT][GA]AG
SnaBI 325 TACGTA TAC^GTA
TaqI 89 TCGA T^CGA
TfiI 337 GATTC G^A[AT]TC
Tsp45I 154 GTCAC ^GT[GC]AC
TthHB8I 89 TCGA T^CGA
XcmI 200 CCAGCCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 107 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 324 GTAC GT^AC
328 GTAC
BanII 93 GGGCTC G[GA]GC[CT]^C
214 GAGCTC
BcgI 288 CGAAGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
259 GCAACACGCTCG
BmyI 93 GGGCTC G[AGT]GC[ACT]^C
214 GAGCTC
BpmI 169 CTCCAG CTGGAG(16/14)
319 CTCCAG
Bsp1286I 93 GGGCTC G[AGT]GC[ACT]^C
214 GAGCTC
BssKI 159 CCAGG ^CC[ACGT]GG
231 CCAGG
BstNI 159 CCAGG CC^[AT]GG
231 CCAGG
DpnII 108 GATC ^GATC
147 GATC
DsaI 134 CCACGG C^C[GA][CT]GG
209 CCATGG
EcoNI 144 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
168 CCTCCAGAAGG
EcoRII 159 CCAGG ^CC[AT]GG
231 CCAGG
HaeIII 356 GGCC GG^CC
363 GGCC
MboI 108 GATC ^GATC
147 GATC
MvaI 159 CCAGG CC^[AT]GG
231 CCAGG
MwoI 6 GCCGGTGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
57 GCGCGTGCTGC
NdeII 108 GATC ^GATC
147 GATC
PflMI 171 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCAGACCATGG
RsaI 324 GTAC GT^AC
328 GTAC
Sau3AI 108 GATC ^GATC
147 GATC
ScrFI 159 CCAGG CC^[ACGT]GG
231 CCAGG
Van91I 171 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCAGACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI AvaII BbsI BbvI
BpuAI BsaJI BsiYI BslI BstUI
Cac8I CfoI Cfr13I EcoT14I FauI
Fnu4HI HhaI HinP1I ItaI MboII
MnlI MvnI NlaIV Sau96I StyI
TseI