Restriction map for
pFN21AE5330
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MfeI MluI MroI MseI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 161 GGTACC G^GTACC
Alw44I 319 GTGCAC G^TGCAC
ApaLI 319 GTGCAC G^TGCAC
Asp718I 161 GGTACC G^GTACC
AspHI 319 GTGCAC G[AT]GC[AT]^C
BalI 153 TGGCCA TGG^CCA
BbsI 588 GAAGAC GAAGAC(2/6)
BfaI 80 CTAG C^TAG
BpuAI 588 GAAGAC GAAGAC(2/6)
BsaWI 646 TCCGGT [AT]^CCGG[AT]
BseRI 228 CTCCTC GAGGAG(10/8)
BsiEI 678 CGGCCG CG[GA][CT]^CG
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
BsmBI 390 CGTCTC CGTCTC(1/5)
BsmFI 180 GTCCC GGGAC(10/14)
BsrDI 363 CATTGC GCAATG(2/0)
BstF5I 83 GGATG GGATG(2/0)
BstXI 23 CCACTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 67 GCGC GCG^C
EagI 678 CGGCCG C^GGCCG
EclXI 678 CGGCCG C^GGCCG
Eco52I 678 CGGCCG C^GGCCG
EcoNI 516 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 720 CCTTGG C^C[AT][AT]GG
FauI 759 GCGGG CCCGC(4/6)
FokI 83 GGATG GGATG(9/13)
HhaI 67 GCGC GCG^C
HinP1I 67 GCGC G^CGC
KpnI 161 GGTACC GGTAC^C
MaeI 80 CTAG C^TAG
MaeIII 469 GTCAC ^GT[ACGT]AC
MluNI 153 TGGCCA TGG^CCA
MscI 153 TGGCCA TGG^CCA
MslI 24 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 433 GCCGGC GCC^GGC
NgoMIV 433 GCCGGC G^CCGGC
NlaIII 694 CATG CATG^
SexAI 341 ACCAGGT A^CC[AT]GGT
StyI 720 CCTTGG C^C[AT][AT]GG
Tsp45I 469 GTCAC ^GT[GC]AC
Tsp509I 787 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 141 GTAC GT^AC
162 GTAC
BanI 161 GGTACC G^G[CT][GA]CC
436 GGCACC
BanII 38 GAGCCC G[GA]GC[CT]^C
500 GGGCTC
BsaJI 121 CCCTGG C^C[ACGT][ACGT]GG
720 CCTTGG
BstYI 236 GGATCT [GA]^GATC[CT]
407 AGATCC
DdeI 43 CTCAG C^T[ACGT]AG
308 CTAAG
HinfI 280 GAGTC G^A[ACGT]TC
643 GACTC
MflI 236 GGATCT [GA]^GATC[CT]
407 AGATCC
PflMI 110 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
191 CCAACAGCTGG
PleI 643 GACTC GAGTC(4/5)
280 GAGTC
PstI 136 CTGCAG CTGCA^G
487 CTGCAG
RsaI 141 GTAC GT^AC
162 GTAC
SfcI 136 CTGCAG C^T[GA][CT]AG
487 CTGCAG
Van91I 110 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
191 CCAACAGCTGG
XhoII 236 GGATCT [GA]^GATC[CT]
407 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BmyI BpmI BsaI BsiYI
BslI BsmAI Bsp1286I BspMI BsrFI
BsrI BssKI BstNI BstUI Cac8I
Cfr10I Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIV PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
TseI TspRI