(Circular) (Six-base) MAPSORT of: pFN21AE5330.seq Check: 6771 from: 1 to: 5512 pFN21AE5330 5512 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2183 2567 2645 2183 Size: 384 78 5050 Fragments arranged by size: 5050 384 78 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2166 2833 2166 Size: 667 4845 AclI AA'CG_TT Cuts at: 1563 2811 3639 4012 1563 Size: 1248 828 373 3063 Fragments arranged by size: 3063 1248 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2543 3575 4959 5227 1632 Size: 911 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 911 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 4645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaLI G'TGCA_C Cuts at: 2324 3569 4725 2324 Size: 1245 1156 3111 Fragments arranged by size: 3111 1245 1156 ApoI r'AATT_y Cuts at: 1493 2821 3047 3199 5308 1493 Size: 1328 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2837 1870 Size: 73 894 4545 Fragments arranged by size: 4545 894 73 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2441 2833 4288 618 Size: 325 217 627 379 275 392 1455 1842 Fragments arranged by size: 1842 1455 627 392 379 325 275 217 BanII G_rGCy'C Cuts at: 1391 2047 2509 2831 1391 Size: 656 462 322 4072 Fragments arranged by size: 4072 656 462 322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2600 928 Size: 971 701 3840 Fragments arranged by size: 3840 971 701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3703 3737 1903 Size: 34 1766 34 3678 Fragments arranged by size: 3678 1766 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4123 136 Size: 107 122 71 631 605 2451 1525 Fragments arranged by size: 2451 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 2328 3573 4729 1790 Size: 538 1245 1156 2573 Fragments arranged by size: 2573 1245 1156 538 BmgBI CAC'GTC Cuts at: 1318 2718 1318 Size: 1400 4112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3243 4201 442 Size: 416 53 83 399 24 1826 958 1753 Fragments arranged by size: 1826 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2308 2308 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2221 2548 4172 1239 Size: 822 160 327 1624 2579 Fragments arranged by size: 2579 1624 822 327 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2065 2140 2356 2773 4175 882 Size: 617 566 75 216 417 1402 2219 Fragments arranged by size: 2219 1402 617 566 417 216 75 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3701 4336 275 Size: 53 83 186 936 1786 382 635 1451 Fragments arranged by size: 1786 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2651 3945 4343 4617 4764 5175 2651 Size: 1294 398 274 147 411 2988 Fragments arranged by size: 2988 1294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2336 2354 2366 2381 Size: 30 165 30 921 12 18 12 15 Cuts at: 2381 2411 2633 2663 2735 2765 1178 Size: 30 222 30 72 30 3925 Fragments arranged by size: 3925 921 222 165 72 30 30 30 30 30 18 15 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 1186 Size: 1038 4474 BseYI C'CCAG_C Cuts at: 2202 2257 2481 2612 4715 5211 2202 Size: 55 224 131 2103 496 2503 Fragments arranged by size: 2503 2103 496 224 131 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4939 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2686 2962 3723 4751 1540 Size: 275 186 685 276 761 1028 2301 Fragments arranged by size: 2301 1028 761 685 276 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3573 3658 4729 2328 Size: 1245 85 1071 3111 Fragments arranged by size: 3111 1245 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 BsmBI CGTCTCn'nnnn_ Cuts at: 2401 2401 Size: 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2328 2509 2831 3573 3658 4729 Size: 399 257 281 181 322 742 85 1071 Cuts at: 4729 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 322 281 257 181 85 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2183 2567 2645 2867 844 Size: 1278 61 384 78 222 3489 Fragments arranged by size: 3489 1278 384 222 78 61 BsrBI CCG'CTC Cuts at: 1394 1823 3399 1394 Size: 429 1576 3507 Fragments arranged by size: 3507 1576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 4007 4181 65 Size: 1183 1119 1640 174 1396 Fragments arranged by size: 1640 1396 1183 1119 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 2532 2685 4156 4343 1700 Size: 110 628 94 153 1471 187 2869 Fragments arranged by size: 2869 1471 628 187 153 110 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5512 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5512 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 5512 BstYI r'GATC_y Cuts at: 1972 2241 2412 2842 3209 3594 3611 5063 5149 Size: 269 171 430 367 385 17 1452 86 Cuts at: 5149 5161 5507 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 430 385 367 346 269 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4960 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4773 Fragments arranged by size: 4773 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3028 3822 3842 2221 Size: 807 794 20 3891 Fragments arranged by size: 3891 807 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2816 2816 Size: 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2298 2683 Size: 54 1425 50 275 18 328 140 385 Cuts at: 2683 2959 3848 5477 8 Size: 276 889 1629 43 Fragments arranged by size: 1629 1425 889 385 328 276 275 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2683 2959 1537 Size: 275 871 276 4090 Fragments arranged by size: 4090 871 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3442 1353 Size: 2089 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4099 4473 4619 387 Size: 1064 138 2510 374 146 1280 Fragments arranged by size: 2510 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2221 2543 2548 3575 4172 4959 Size: 393 429 160 322 5 1027 597 787 Cuts at: 4959 5227 1239 Size: 268 1524 Fragments arranged by size: 1524 1027 787 597 429 393 322 268 160 5 EcoNI CCTnn'n_nnAGG Cuts at: 2525 2525 Size: 5512 EcoO109I rG'GnC_Cy Cuts at: 2013 2184 2206 2013 Size: 171 22 5319 Fragments arranged by size: 5319 171 22 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FspI TGC'GCA Cuts at: 4018 4018 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 4659 1678 Size: 2981 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 4153 4185 4227 Size: 32 135 32 752 32 1797 32 42 Cuts at: 4227 4259 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 752 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3102 669 Size: 988 281 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 3102 3102 Size: 5512 KpnI G_GTAC'C Cuts at: 2170 2837 2170 Size: 667 4845 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 2796 4626 4810 1305 Size: 1062 429 1830 184 2007 Fragments arranged by size: 2007 1830 1062 429 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2160 10 Size: 54 1425 343 328 3362 Fragments arranged by size: 3362 1425 343 328 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 3470 3829 3988 518 Size: 648 867 1437 359 159 2042 Fragments arranged by size: 2042 1437 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2121 2202 2419 2662 2928 3605 4753 4998 Size: 515 81 217 243 266 677 1148 245 Cuts at: 4998 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 515 266 245 243 217 81 NaeI GCC'GGC Cuts at: 2440 2440 Size: 5512 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4960 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NgoMIV G'CCGG_C Cuts at: 2438 2438 Size: 5512 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2959 2959 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 2121 2202 1108 Size: 1013 81 4418 Fragments arranged by size: 4418 1013 81 PfoI T'CCnGG_A Cuts at: 2237 2237 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2013 2184 2206 2013 Size: 171 22 5319 Fragments arranged by size: 5319 171 22 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PstI C_TGCA'G Cuts at: 830 2145 2496 2864 830 Size: 1315 351 368 3478 Fragments arranged by size: 3478 1315 368 351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 PvuII CAG'CTG Cuts at: 2121 2202 2419 2121 Size: 81 217 5214 Fragments arranged by size: 5214 217 81 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SanDI GG'GwC_CC Cuts at: 2184 2184 Size: 5512 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SexAI A'CCwGG_T Cuts at: 2346 2346 Size: 5512 SfcI C'TryA_G Cuts at: 826 1045 2141 2492 2860 3995 4676 4867 826 Size: 219 1096 351 368 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 681 368 351 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3616 4517 4779 820 Size: 197 926 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2725 2951 513 Size: 552 1660 226 3074 Fragments arranged by size: 3074 1660 552 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2710 3539 3556 3709 3894 4339 325 Size: 1255 1130 829 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1130 829 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3758 5470 Size: 274 80 33 51 1443 168 1613 1712 Cuts at: 5470 96 Size: 138 Fragments arranged by size: 1712 1613 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5472 5504 5472 Size: 32 5480 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI