(Linear) (Six-base) MAPSORT of: KIEE5330.seq Check: 3194 from: 1 to: 807 KIEE5330.orf With 182 enzymes: SgfI * January 29, 2014 11:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 178 562 640 807 Size: 178 384 78 167 Fragments arranged by size: 384 178 167 78 Acc65I G'GTAC_C Cuts at: 0 161 807 Size: 161 646 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 807 Size: 538 269 AleI CACnn'nnGTG Cuts at: 0 28 807 Size: 28 779 ApaLI G'TGCA_C Cuts at: 0 319 807 Size: 319 488 BanI G'GyrC_C Cuts at: 0 161 436 807 Size: 161 275 371 Fragments arranged by size: 371 275 161 BanII G_rGCy'C Cuts at: 0 42 504 807 Size: 42 462 303 Fragments arranged by size: 462 303 42 BbsI GAAGACnn'nnnn_ Cuts at: 0 595 807 Size: 595 212 Bme1580I G_kGCm'C Cuts at: 0 323 807 Size: 323 484 BmgBI CAC'GTC Cuts at: 0 713 807 Size: 713 94 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 303 807 Size: 303 504 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 216 543 807 Size: 56 160 327 264 Fragments arranged by size: 327 264 160 56 BsaI GGTCTCn'nnnn_ Cuts at: 0 60 135 351 768 807 Size: 60 75 216 417 39 Fragments arranged by size: 417 216 75 60 39 BsaWI w'CCGG_w Cuts at: 0 646 807 Size: 646 161 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 319 331 349 361 376 406 628 658 Size: 319 12 18 12 15 30 222 30 Cuts at: 658 730 760 807 Size: 72 30 47 Fragments arranged by size: 319 222 72 47 30 30 30 18 15 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 219 807 Size: 219 588 BseYI C'CCAG_C Cuts at: 0 197 252 476 607 807 Size: 197 55 224 131 200 Fragments arranged by size: 224 200 197 131 55 BsiEI CG_ry'CG Cuts at: 0 681 807 Size: 681 126 BsiHKAI G_wGCw'C Cuts at: 0 323 807 Size: 323 484 BsmBI CGTCTCn'nnnn_ Cuts at: 0 396 807 Size: 396 411 Bsp1286I G_dGCh'C Cuts at: 0 42 323 504 807 Size: 42 281 181 303 Fragments arranged by size: 303 281 181 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 178 562 640 807 Size: 117 61 384 78 167 Fragments arranged by size: 384 167 117 78 61 BsrDI GCAATG_nn' Cuts at: 0 362 807 Size: 362 445 BsrFI r'CCGG_y Cuts at: 0 433 527 680 807 Size: 433 94 153 127 Fragments arranged by size: 433 153 127 94 BstXI CCAn_nnnn'nTGG Cuts at: 0 30 807 Size: 30 777 BstYI r'GATC_y Cuts at: 0 236 407 807 Size: 236 171 400 Fragments arranged by size: 400 236 171 BtsI GCAGTG_nn' Cuts at: 0 216 807 Size: 216 591 EaeI y'GGCC_r Cuts at: 0 153 293 678 807 Size: 153 140 385 129 Fragments arranged by size: 385 153 140 129 EagI C'GGCC_G Cuts at: 0 678 807 Size: 678 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 216 538 543 807 Size: 56 160 322 5 264 Fragments arranged by size: 322 264 160 56 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 520 807 Size: 520 287 EcoO109I rG'GnC_Cy Cuts at: 0 8 179 201 807 Size: 8 171 22 606 Fragments arranged by size: 606 171 22 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 319 351 807 Size: 319 32 456 Fragments arranged by size: 456 319 32 KpnI G_GTAC'C Cuts at: 0 165 807 Size: 165 642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 362 791 807 Size: 362 429 16 Fragments arranged by size: 429 362 16 MscI TGG'CCA Cuts at: 0 155 807 Size: 155 652 MslI CAynn'nnrTG Cuts at: 0 28 807 Size: 28 779 MspA1I CmG'CkG Cuts at: 0 116 197 414 657 807 Size: 116 81 217 243 150 Fragments arranged by size: 243 217 150 116 81 NaeI GCC'GGC Cuts at: 0 435 807 Size: 435 372 NgoMIV G'CCGG_C Cuts at: 0 433 807 Size: 433 374 PflMI CCAn_nnn'nTGG Cuts at: 0 116 197 807 Size: 116 81 610 Fragments arranged by size: 610 116 81 PfoI T'CCnGG_A Cuts at: 0 232 807 Size: 232 575 PpuMI rG'GwC_Cy Cuts at: 0 8 179 201 807 Size: 8 171 22 606 Fragments arranged by size: 606 171 22 8 PstI C_TGCA'G Cuts at: 0 140 491 807 Size: 140 351 316 Fragments arranged by size: 351 316 140 PvuII CAG'CTG Cuts at: 0 116 197 414 807 Size: 116 81 217 393 Fragments arranged by size: 393 217 116 81 SanDI GG'GwC_CC Cuts at: 0 179 807 Size: 179 628 SexAI A'CCwGG_T Cuts at: 0 341 807 Size: 341 466 SfcI C'TryA_G Cuts at: 0 136 487 807 Size: 136 351 320 Fragments arranged by size: 351 320 136 StyI C'CwwG_G Cuts at: 0 720 807 Size: 720 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 705 807 Size: 705 102 TatI w'GTAC_w Cuts at: 0 140 807 Size: 140 667 Enzymes that do cut: AarI Acc65I AcuI AleI ApaLI BanI BanII BbsI Bme1580I BmgBI BplI BpmI BsaI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtsI EaeI EagI Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV PflMI PfoI PpuMI PstI PvuII SanDI SexAI SfcI StyI TaqII TatI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI