################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BbsI BcgI BclI BfaI BfrI BglII BlnI BpuAI BseAI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MfeI MflI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 2189 AAATTC [GA]^AATT[CT] AflIII 659 ACGTGT A^C[GA][CT]GT AgeI 434 ACCGGT A^CCGGT AhdI 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1932 GTGCAC G^TGCAC AlwI 3 GATCC GGATC(4/5) ApaLI 1932 GTGCAC G^TGCAC ApoI 2189 AAATTC [GA]^AATT[CT] AspEI 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1529 GGCGCC GGCGC^C BbvCI 1800 CCTCAGC CCTCAGC(-5/-2) BciVI 733 GGATAC GTATCC(6/5) BlpI 2037 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2037 GCTGAGC GC^T[ACGT]AGC BsaBI 1960 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 434 ACCGGT [AT]^CCGG[AT] BsiWI 317 CGTACG C^GTACG BssHII 1503 GCGCGC G^CGCGC BssSI 2140 CTCGTG CACGAG(-5/-1) BstAPI 1571 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 822 GGTCACC G^GT[ACGT]ACC BstPI 822 GGTCACC G^GT[ACGT]ACC BstXI 1239 CCAGGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2037 GCTGAGC GC^T[ACGT]AGC DpnII 3 GATC ^GATC DrdI 110 GACACCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 948 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 822 GGTCACC G^GT[ACGT]ACC HincII 1582 GTCAAC GT[CT]^[GA]AC HindII 1582 GTCAAC GT[CT]^[GA]AC KasI 1529 GGCGCC G^GCGCC MamI 1960 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 3 GATC ^GATC MslI 2016 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1529 GGCGCC GG^CGCC NdeII 3 GATC ^GATC NotI 879 GCGGCCGC GC^GGCCGC PflFI 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1587 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 434 ACCGGT A^CCGGT PshAI 849 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 3 GATC ^GATC ScaI 2048 AGTACT AGT^ACT SfiI 872 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1529 GGCGCC GGC^GCC SmaI 1214 CCCGGG CCC^GGG StuI 687 AGGCCT AGG^CCT Tsp509I 2190 AATT ^AATT Tth111I 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1587 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 58 CCACAGGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1214 CCCGGG C^CCGGG XmaI 1214 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 1504 CGCG CG^CG 1506 CGCG AcyI 461 GACGCC G[GA]^CG[CT]C 1529 GGCGCC AvaI 93 CTCGGG C^[CT]CG[GA]G 1214 CCCGGG BsaHI 461 GACGCC G[GA]^CG[CT]C 1529 GGCGCC BsgI 232 CTGCAC GTGCAG(16/14) 663 GTGCAG BsiEI 880 CGGCCG CG[GA][CT]^CG 2099 CGGCCG BsmBI 310 GAGACG CGTCTC(1/5) 459 GAGACG BsmI 164 GCATTC GAATGC(1/-1) 275 GCATTC BsoBI 93 CTCGGG C^[CT]CG[GA]G 1214 CCCGGG BsrFI 434 ACCGGT [GA]^CCGG[CT] 2222 ACCGGC BstUI 1504 CGCG CG^CG 1506 CGCG Cfr10I 434 ACCGGT [GA]^CCGG[CT] 2222 ACCGGC DraIII 195 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 1926 CACCCTGTG EagI 880 CGGCCG C^GGCCG 2099 CGGCCG EclXI 880 CGGCCG C^GGCCG 2099 CGGCCG Eco52I 880 CGGCCG C^GGCCG 2099 CGGCCG Hin1I 461 GACGCC G[GA]^CG[CT]C 1529 GGCGCC MvnI 1504 CGCG CG^CG 1506 CGCG NcoI 305 CCATGG C^CATGG 1721 CCATGG NspI 1553 ACATGC [GA]CATG^[CT] 1736 GCATGT PleI 622 GACTC GAGTC(4/5) 800 GAGTC SapI 909 GAAGAGC GCTCTTC(1/4) 1623 GCTCTTC SmlI 208 CTTGAG C^T[CT][GA]AG 444 CTCAAG TaqI 290 TCGA T^CGA 1610 TCGA TthHB8I 290 TCGA T^CGA 1610 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PpuMI PstI RsaI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI