Restriction map for
pFN21AE2436
(Circular) (Six-base) MAPSORT of: pFN21AE2436.seq Check: 163 from: 1 to: 6949
pFN21AE2436 6949 bp
With 182 enzymes: SgfI *
November 22, 2012 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3436 3436
Size: 6949
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 3421 3742 5012 6396 6664 1632
Size: 1745 44 321 1270 1384 268 1917
Fragments arranged by size:
1917 1745 1384 1270 321 268 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 2664 5848 6825 1203
Size: 1461 3184 977 1327
Fragments arranged by size:
3184 1461 1327 977
AgeI A'CCGG_T
Cuts at: 2439 5780 2439
Size: 3341 3608
AhdI GACnn_n'nnGTC
Cuts at: 3438 5678 3438
Size: 2240 4709
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AlwNI CAG_nnn'CTG
Cuts at: 2419 3523 4072 4692 6264 2419
Size: 1104 549 620 1572 3104
Fragments arranged by size:
3104 1572 1104 620 549
ApaI G_GGCC'C
Cuts at: 2788 3226 4095 2788
Size: 438 869 5642
Fragments arranged by size:
5642 869 438
ApaLI G'TGCA_C
Cuts at: 3937 5006 6162 3937
Size: 1069 1156 4724
Fragments arranged by size:
4724 1156 1069
ApoI r'AATT_y
Cuts at: 1493 4194 4258 4484 4636 6745 1493
Size: 2701 64 226 152 2109 1697
Fragments arranged by size:
2701 2109 1697 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 3219 4274 1870
Size: 73 155 1121 1055 4545
Fragments arranged by size:
4545 1121 1055 155 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2428 2461 2428
Size: 33 6916
BamHI G'GATC_C
Cuts at: 4279 4646 4279
Size: 367 6582
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3275 3534 4007 4270 5725
Size: 325 217 627 1488 259 473 263 1455
Cuts at: 5725 618
Size: 1842
Fragments arranged by size:
1842 1488 1455 627 473 325 263 259
217
BanII G_rGCy'C
Cuts at: 1391 2788 2921 3226 3320 3500 4095 4268 1391
Size: 1397 133 305 94 180 595 173 4072
Fragments arranged by size:
4072 1397 595 305 180 173 133 94
BbeI G_GCGC'C
Cuts at: 3538 3538
Size: 6949
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5978
BbvCI CC'TCA_GC
Cuts at: 3806 3806
Size: 6949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2732 4841 6051 2732
Size: 2109 1210 3630
Fragments arranged by size:
3630 2109 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 2714 2884
Size: 107 122 71 631 605 958 84 170
Cuts at: 2884 5560 136
Size: 2676 1525
Fragments arranged by size:
2676 1525 958 631 605 170 122 107
84 71
BglII A'GATC_T
Cuts at: 6944 6944
Size: 6949
BlpI GC'TnA_GC
Cuts at: 4043 4366 4043
Size: 323 6626
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2500 2788 3226 3280 3941 4095 5010
Size: 420 290 288 438 54 661 154 915
Cuts at: 5010 6166 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 915 661 438 420 290 288
154 54
BmgBI CAC'GTC
Cuts at: 1318 2557 2665 1318
Size: 1239 108 5602
Fragments arranged by size:
5602 1239 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2770 4680 5638
Size: 416 53 83 399 24 1353 1910 958
Cuts at: 5638 442
Size: 1753
Fragments arranged by size:
1910 1753 1353 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2060 3115 2060
Size: 1055 5894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2992 3281 3484 3806 3815 3859 4123
Size: 856 897 289 203 322 9 44 264
Cuts at: 4123 5609 1239
Size: 1486 2579
Fragments arranged by size:
2579 1486 897 856 322 289 264 203
44 9
Bpu10I CC'TnA_GC
Cuts at: 2834 3806 2834
Size: 972 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2434 5074 5939 6237 2234
Size: 200 2640 865 298 2946
Fragments arranged by size:
2946 2640 865 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2726 3355 4081 5612 882
Size: 617 1227 629 726 1531 2219
Fragments arranged by size:
2219 1531 1227 726 629 617
BsaAI yAC'GTr
Cuts at: 493 1204 2326 2743 3889 4740 493
Size: 711 1122 417 1146 851 2702
Fragments arranged by size:
2702 1146 1122 851 711 417
BsaBI GATnn'nnATC
Cuts at: 3969 6943 3969
Size: 2974 3975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2467 3535 4756 5138
Size: 53 83 186 936 934 1068 1221 382
Cuts at: 5138 5773 275
Size: 635 1451
Fragments arranged by size:
1451 1221 1068 936 934 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2439 5382 5780 6054 6201 6612 2439
Size: 2943 398 274 147 411 2776
Fragments arranged by size:
2943 2776 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 5791
Fragments arranged by size:
5791 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2173 2742 3411 3812 4162 4235 1186
Size: 987 569 669 401 350 73 3900
Fragments arranged by size:
3900 987 669 569 401 350 73
BseYI C'CCAG_C
Cuts at: 2051 2503 2642 2921 3026 3735 4202 6152 6648
Size: 452 139 279 105 709 467 1950 496
Cuts at: 6648 2051
Size: 2352
Fragments arranged by size:
2352 1950 709 496 467 452 279 139
105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2689 1163
Size: 573 486 467 5423
Fragments arranged by size:
5423 573 486 467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2888 4107 4399 5160 6188 1540
Size: 275 186 887 1219 292 761 1028 2301
Fragments arranged by size:
2301 1219 1028 887 761 292 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 3941 4227 5010 5095 6166 2340
Size: 1601 286 783 85 1071 3123
Fragments arranged by size:
3123 1601 1071 783 286 85
BsiWI C'GTAC_G
Cuts at: 2322 2322
Size: 6949
BsmI GAATG_Cn'
Cuts at: 2169 2280 4460 4553 2169
Size: 111 2180 93 4565
Fragments arranged by size:
4565 2180 111 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2309 2458 2309
Size: 149 6800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2340 2500 2788 2921 3226 3280
Size: 399 420 130 160 288 133 305 54
Cuts at: 3280 3320 3500 3941 4095 4227 4268 5010 5095
Size: 40 180 441 154 132 41 742 85
Cuts at: 5095 6166 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 441 420 399 305 288
180 160 154 133 132 130 85 54
41 40
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 4838 6568 4838
Size: 1730 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2708 3436 4304 844
Size: 1400 464 728 868 3489
Fragments arranged by size:
3489 1400 868 728 464
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5444 5618 65
Size: 1183 4196 174 1396
Fragments arranged by size:
4196 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2439 4227 5593 5780 1700
Size: 110 629 1788 1366 187 2869
Fragments arranged by size:
2869 1788 1366 629 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6949
BssHII G'CGCG_C
Cuts at: 1925 3508 1925
Size: 1583 5366
BssSI C'ACGA_G
Cuts at: 4145 5009 6021 4145
Size: 864 1012 5073
Fragments arranged by size:
5073 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3582 794
Size: 2788 4161
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 5306
BstXI CCAn_nnnn'nTGG
Cuts at: 3251 3251
Size: 6949
BstYI r'GATC_y
Cuts at: 1972 4279 4646 5031 5048 6500 6586 6598 6944
Size: 2307 367 385 17 1452 86 12 346
Cuts at: 6944 1972
Size: 1977
Fragments arranged by size:
2307 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2310 3250 3528 3726 4107 513
Size: 552 1245 940 278 198 381 3355
Fragments arranged by size:
3355 1245 940 552 381 278 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2527 3814 487
Size: 683 56 1301 1287 3622
Fragments arranged by size:
3622 1301 1287 683 56
BtsI GCAGTG_nn'
Cuts at: 2498 2820 4120 4465 5259 5279 2498
Size: 322 1300 345 794 20 4168
Fragments arranged by size:
4168 1300 794 345 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DraIII CAC_nnn'GTG
Cuts at: 2205 3936 2205
Size: 1731 5218
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2121 5956 809
Size: 507 805 3835 1802
Fragments arranged by size:
3835 1802 805 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 2885 2994
Size: 54 1425 50 275 18 697 358 109
Cuts at: 2994 3114 3154 3171 3375 3450 4104 4396 5285
Size: 120 40 17 204 75 654 292 889
Cuts at: 5285 6914 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 697 654 358 292 275
204 120 109 75 54 50 43 40
18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2885 4104 4396 1537
Size: 275 1073 1219 292 4090
Fragments arranged by size:
4090 1219 1073 292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2822 2909 3353 3635 4879 1353
Size: 698 771 87 444 282 1244 3423
Fragments arranged by size:
3423 1244 771 698 444 282 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2648 2766 5536 5910 6056 387
Size: 1064 138 1059 118 2770 374 146 1280
Fragments arranged by size:
2770 1280 1064 1059 374 146 138 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2992 3281 3377 3421 3484 3742
Size: 393 463 897 289 96 44 63 258
Cuts at: 3742 3806 3815 3859 4123 5012 5609 6396 6664
Size: 64 9 44 264 889 597 787 268
Cuts at: 6664 1239
Size: 1524
Fragments arranged by size:
1524 897 889 787 597 463 393 289
268 264 258 96 64 63 44 44
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2957 2957
Size: 6949
EcoO109I rG'GnC_Cy
Cuts at: 2079 2233 2250 2578 2784 2785 2966 3223 3404
Size: 154 17 328 206 1 181 257 181
Cuts at: 3404 3670 3679 4091 4092 2079
Size: 266 9 412 1 4936
Fragments arranged by size:
4936 412 328 266 257 206 181 181
154 17 9 1 1
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 2625 3538 3572 6096 1678
Size: 947 913 34 2524 2531
Fragments arranged by size:
2531 2524 947 913 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2730 2762 3424 3456 5590
Size: 32 135 32 1158 32 662 32 2134
Cuts at: 5590 5622 5664 5696 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2134 1158 662 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3589 4293 4539 669
Size: 988 281 1651 704 246 3079
Fragments arranged by size:
3079 1651 988 704 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6949
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KasI G'GCGC_C
Cuts at: 3534 3534
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6063 6247 1305
Size: 4758 184 2007
Fragments arranged by size:
4758 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3173 3377 3452 10
Size: 54 1425 343 1341 204 75 3507
Fragments arranged by size:
3507 1425 1341 343 204 75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4025 4907 5266 5425 518
Size: 648 2859 882 359 159 2042
Fragments arranged by size:
2859 2042 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2881 2932 3717 4365 5042 6190 6435 1606
Size: 1275 51 785 648 677 1148 245 2120
Fragments arranged by size:
2120 1275 1148 785 677 648 245 51
NarI GG'CG_CC
Cuts at: 3535 3535
Size: 6949
NcoI C'CATG_G
Cuts at: 513 1065 2310 3726 513
Size: 552 1245 1416 3736
Fragments arranged by size:
3736 1416 1245 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 2885 4396 2885
Size: 1511 5438
NspI r_CATG'y
Cuts at: 1132 1229 3562 3745 4307 5852 1132
Size: 97 2333 183 562 1545 2229
Fragments arranged by size:
2333 2229 1545 562 183 97
PasI CC'CwG_GG
Cuts at: 1348 2788 1348
Size: 1440 5509
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3598 1108
Size: 2490 4459
PfoI T'CCnGG_A
Cuts at: 2256 3381 3783 3875 2256
Size: 1125 402 92 5330
Fragments arranged by size:
5330 1125 402 92
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2233 2250 3404 3670 2079
Size: 154 17 1154 266 5358
Fragments arranged by size:
5358 1154 266 154 17
PshAI GACnn'nnGTC
Cuts at: 1613 2858 1613
Size: 1245 5704
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PspOMI G'GGCC_C
Cuts at: 2784 3222 4091 2784
Size: 438 869 5642
Fragments arranged by size:
5642 869 438
PstI C_TGCA'G
Cuts at: 830 2346 2415 4216 4301 830
Size: 1516 69 1801 85 3478
Fragments arranged by size:
3478 1801 1516 85 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SanDI GG'GwC_CC
Cuts at: 3404 3670 3404
Size: 266 6683
SapI GCTCTTCn'nnn_
Cuts at: 2909 3635 2909
Size: 726 6223
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 4055 5197 6909 4055
Size: 1142 1712 4095
Fragments arranged by size:
4095 1712 1142
SfcI C'TryA_G
Cuts at: 826 1045 2342 2368 2411 2490 4212 4297 5432
Size: 219 1297 26 43 79 1722 85 1135
Cuts at: 5432 6113 6304 826
Size: 681 191 1471
Fragments arranged by size:
1722 1471 1297 1135 681 219 191 85
79 43 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 2884 2884
Size: 6949
SfoI GGC'GCC
Cuts at: 3536 3536
Size: 6949
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 3221 4276 1872
Size: 1349 1055 4545
Fragments arranged by size:
4545 1349 1055
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2449 5053 5954 6216 820
Size: 197 926 270 236 2604 901 262 1553
Fragments arranged by size:
2604 1553 926 901 270 262 236 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 4873 5
Size: 47 4821 2081
Fragments arranged by size:
4821 2081 47
StuI AGG'CCT
Cuts at: 1517 2694 1517
Size: 1177 5772
StyI C'CwwG_G
Cuts at: 513 1065 2310 3321 3372 3564 3675 3726 4388
Size: 552 1245 1011 51 192 111 51 662
Cuts at: 4388 513
Size: 3074
Fragments arranged by size:
3074 1245 1011 662 552 192 111 51
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2414 2703 4976 4993 5146 5331 5776
Size: 1255 834 289 2273 17 153 185 445
Cuts at: 5776 325
Size: 1498
Fragments arranged by size:
2273 1498 1255 834 445 289 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3708 4053 4131
Size: 274 80 33 51 1443 1731 345 78
Cuts at: 4131 4167 5195 6907 96
Size: 36 1028 1712 138
Fragments arranged by size:
1731 1712 1443 1028 345 274 138 80
78 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6909 6941 6909
Size: 32 6917
Tth111I GACn'n_nGTC
Cuts at: 1316 3128 1316
Size: 1812 5137
XbaI T'CTAG_A
Cuts at: 4285 4285
Size: 6949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2070 2070
Size: 6949
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6949
XmaI C'CCGG_G
Cuts at: 1870 3219 4274 1870
Size: 1349 1055 4545
Fragments arranged by size:
4545 1349 1055
XmnI GAAnn'nnTTC
Cuts at: 5078 5078
Size: 6949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SexAI SrfI SwaI