Restriction map for
pFN21AE2436
(Linear) (Six-base) MAPSORT of: KIEE2436.seq Check: 2670 from: 1 to: 2244
KIEE2436.orf
With 182 enzymes: SgfI *
November 22, 2012 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1431 2244
Size: 1431 813
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1416 1737 2244
Size: 1372 44 321 507
Fragments arranged by size:
1372 507 321 44
AflIII A'CryG_T
Cuts at: 0 659 2244
Size: 659 1585
AgeI A'CCGG_T
Cuts at: 0 434 2244
Size: 434 1810
AhdI GACnn_n'nnGTC
Cuts at: 0 1433 2244
Size: 1433 811
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1518 2067 2244
Size: 414 1104 549 177
Fragments arranged by size:
1104 549 414 177
ApaI G_GGCC'C
Cuts at: 0 783 1221 2090 2244
Size: 783 438 869 154
Fragments arranged by size:
869 783 438 154
ApaLI G'TGCA_C
Cuts at: 0 1932 2244
Size: 1932 312
ApoI r'AATT_y
Cuts at: 0 2189 2244
Size: 2189 55
AvaI C'yCGr_G
Cuts at: 0 93 1214 2244
Size: 93 1121 1030
Fragments arranged by size:
1121 1030 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 423 456 2244
Size: 423 33 1788
Fragments arranged by size:
1788 423 33
BanI G'GyrC_C
Cuts at: 0 1270 1529 2002 2244
Size: 1270 259 473 242
Fragments arranged by size:
1270 473 259 242
BanII G_rGCy'C
Cuts at: 0 783 916 1221 1315 1495 2090 2244
Size: 783 133 305 94 180 595 154
Fragments arranged by size:
783 595 305 180 154 133 94
BbeI G_GCGC'C
Cuts at: 0 1533 2244
Size: 1533 711
BbvCI CC'TCA_GC
Cuts at: 0 1801 2244
Size: 1801 443
BciVI GTATCCnnnnn_n'
Cuts at: 0 727 2244
Size: 727 1517
BglI GCCn_nnn'nGGC
Cuts at: 0 625 709 879 2244
Size: 625 84 170 1365
Fragments arranged by size:
1365 625 170 84
BlpI GC'TnA_GC
Cuts at: 0 2038 2244
Size: 2038 206
Bme1580I G_kGCm'C
Cuts at: 0 205 495 783 1221 1275 1936 2090 2244
Size: 205 290 288 438 54 661 154 154
Fragments arranged by size:
661 438 290 288 205 154 154 54
BmgBI CAC'GTC
Cuts at: 0 552 660 2244
Size: 552 108 1584
Fragments arranged by size:
1584 552 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 765 2244
Size: 765 1479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 1110 2244
Size: 55 1055 1134
Fragments arranged by size:
1134 1055 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 987 1276 1479 1801 1810 1854 2118
Size: 90 897 289 203 322 9 44 264
Cuts at: 2118 2244
Size: 126
Fragments arranged by size:
897 322 289 264 203 126 90 44
9
Bpu10I CC'TnA_GC
Cuts at: 0 829 1801 2244
Size: 829 972 443
Fragments arranged by size:
972 829 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 429 2244
Size: 229 200 1815
Fragments arranged by size:
1815 229 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 721 1350 2076 2244
Size: 721 629 726 168
Fragments arranged by size:
726 721 629 168
BsaAI yAC'GTr
Cuts at: 0 321 738 1884 2244
Size: 321 417 1146 360
Fragments arranged by size:
1146 417 360 321
BsaBI GATnn'nnATC
Cuts at: 0 1964 2244
Size: 1964 280
BsaHI Gr'CG_yC
Cuts at: 0 462 1530 2244
Size: 462 1068 714
Fragments arranged by size:
1068 714 462
BsaWI w'CCGG_w
Cuts at: 0 434 2244
Size: 434 1810
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 2244
Size: 301 30 1913
Fragments arranged by size:
1913 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 168 737 1406 1807 2157 2230 2244
Size: 168 569 669 401 350 73 14
Fragments arranged by size:
669 569 401 350 168 73 14
BseYI C'CCAG_C
Cuts at: 0 46 498 637 916 1021 1730 2197 2244
Size: 46 452 139 279 105 709 467 47
Fragments arranged by size:
709 467 452 279 139 105 47 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 684 2244
Size: 217 467 1560
Fragments arranged by size:
1560 467 217
BsiEI CG_ry'CG
Cuts at: 0 883 2102 2244
Size: 883 1219 142
Fragments arranged by size:
1219 883 142
BsiHKAI G_wGCw'C
Cuts at: 0 335 1936 2222 2244
Size: 335 1601 286 22
Fragments arranged by size:
1601 335 286 22
BsiWI C'GTAC_G
Cuts at: 0 317 2244
Size: 317 1927
BsmI GAATG_Cn'
Cuts at: 0 164 275 2244
Size: 164 111 1969
Fragments arranged by size:
1969 164 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 304 453 2244
Size: 304 149 1791
Fragments arranged by size:
1791 304 149
Bsp1286I G_dGCh'C
Cuts at: 0 205 335 495 783 916 1221 1275 1315
Size: 205 130 160 288 133 305 54 40
Cuts at: 1315 1495 1936 2090 2222 2244
Size: 180 441 154 132 22
Fragments arranged by size:
441 305 288 205 180 160 154 133
132 130 54 40 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 703 1431 2244
Size: 239 464 728 813
Fragments arranged by size:
813 728 464 239
BsrFI r'CCGG_y
Cuts at: 0 434 2222 2244
Size: 434 1788 22
Fragments arranged by size:
1788 434 22
BssHII G'CGCG_C
Cuts at: 0 1503 2244
Size: 1503 741
BssSI C'ACGA_G
Cuts at: 0 2140 2244
Size: 2140 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1577 2244
Size: 1577 667
BstEII G'GTnAC_C
Cuts at: 0 822 2244
Size: 822 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1246 2244
Size: 1246 998
BtgI C'CryG_G
Cuts at: 0 305 1245 1523 1721 2102 2244
Size: 305 940 278 198 381 142
Fragments arranged by size:
940 381 305 278 198 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 522 1809 2244
Size: 522 1287 435
Fragments arranged by size:
1287 522 435
BtsI GCAGTG_nn'
Cuts at: 0 493 815 2115 2244
Size: 493 322 1300 129
Fragments arranged by size:
1300 493 322 129
DraIII CAC_nnn'GTG
Cuts at: 0 200 1931 2244
Size: 200 1731 313
Fragments arranged by size:
1731 313 200
DrdI GACnn_nn'nnGTC
Cuts at: 0 116 2244
Size: 116 2128
EaeI y'GGCC_r
Cuts at: 0 522 880 989 1109 1149 1166 1370 1445
Size: 522 358 109 120 40 17 204 75
Cuts at: 1445 2099 2244
Size: 654 145
Fragments arranged by size:
654 522 358 204 145 120 109 75
40 17
EagI C'GGCC_G
Cuts at: 0 880 2099 2244
Size: 880 1219 145
Fragments arranged by size:
1219 880 145
EarI CTCTTCn'nnn_
Cuts at: 0 46 817 904 1348 1630 2244
Size: 46 771 87 444 282 614
Fragments arranged by size:
771 614 444 282 87 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 643 761 2244
Size: 643 118 1483
Fragments arranged by size:
1483 643 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 987 1276 1372 1416 1479 1737 1801
Size: 90 897 289 96 44 63 258 64
Cuts at: 1801 1810 1854 2118 2244
Size: 9 44 264 126
Fragments arranged by size:
897 289 264 258 126 96 90 64
63 44 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 952 2244
Size: 952 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 74 228 245 573 779 780 961 1218
Size: 74 154 17 328 206 1 181 257
Cuts at: 1218 1399 1665 1674 2086 2087 2244
Size: 181 266 9 412 1 157
Fragments arranged by size:
412 328 266 257 206 181 181 157
154 74 17 9 1 1
HaeII r_GCGC'y
Cuts at: 0 620 1533 1567 2244
Size: 620 913 34 677
Fragments arranged by size:
913 677 620 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 1419 1451 2244
Size: 725 32 662 32 793
Fragments arranged by size:
793 725 662 32 32
HincII GTy'rAC
Cuts at: 0 1584 2244
Size: 1584 660
KasI G'GCGC_C
Cuts at: 0 1529 2244
Size: 1529 715
MscI TGG'CCA
Cuts at: 0 1168 1372 1447 2244
Size: 1168 204 75 797
Fragments arranged by size:
1168 797 204 75
MslI CAynn'nnrTG
Cuts at: 0 2020 2244
Size: 2020 224
MspA1I CmG'CkG
Cuts at: 0 876 927 1712 2244
Size: 876 51 785 532
Fragments arranged by size:
876 785 532 51
NarI GG'CG_CC
Cuts at: 0 1530 2244
Size: 1530 714
NcoI C'CATG_G
Cuts at: 0 305 1721 2244
Size: 305 1416 523
Fragments arranged by size:
1416 523 305
NotI GC'GGCC_GC
Cuts at: 0 880 2244
Size: 880 1364
NspI r_CATG'y
Cuts at: 0 1557 1740 2244
Size: 1557 183 504
Fragments arranged by size:
1557 504 183
PasI CC'CwG_GG
Cuts at: 0 783 2244
Size: 783 1461
PflMI CCAn_nnn'nTGG
Cuts at: 0 1593 2244
Size: 1593 651
PfoI T'CCnGG_A
Cuts at: 0 251 1376 1778 1870 2244
Size: 251 1125 402 92 374
Fragments arranged by size:
1125 402 374 251 92
PpuMI rG'GwC_Cy
Cuts at: 0 74 228 245 1399 1665 2244
Size: 74 154 17 1154 266 579
Fragments arranged by size:
1154 579 266 154 74 17
PshAI GACnn'nnGTC
Cuts at: 0 853 2244
Size: 853 1391
PspOMI G'GGCC_C
Cuts at: 0 779 1217 2086 2244
Size: 779 438 869 158
Fragments arranged by size:
869 779 438 158
PstI C_TGCA'G
Cuts at: 0 341 410 2211 2244
Size: 341 69 1801 33
Fragments arranged by size:
1801 341 69 33
SanDI GG'GwC_CC
Cuts at: 0 1399 1665 2244
Size: 1399 266 579
Fragments arranged by size:
1399 579 266
SapI GCTCTTCn'nnn_
Cuts at: 0 904 1630 2244
Size: 904 726 614
Fragments arranged by size:
904 726 614
ScaI AGT'ACT
Cuts at: 0 2050 2244
Size: 2050 194
SfcI C'TryA_G
Cuts at: 0 337 363 406 485 2207 2244
Size: 337 26 43 79 1722 37
Fragments arranged by size:
1722 337 79 43 37 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 879 2244
Size: 879 1365
SfoI GGC'GCC
Cuts at: 0 1531 2244
Size: 1531 713
SmaI CCC'GGG
Cuts at: 0 1216 2244
Size: 1216 1028
SmlI C'TyrA_G
Cuts at: 0 208 444 2244
Size: 208 236 1800
Fragments arranged by size:
1800 236 208
StuI AGG'CCT
Cuts at: 0 689 2244
Size: 689 1555
StyI C'CwwG_G
Cuts at: 0 305 1316 1367 1559 1670 1721 2244
Size: 305 1011 51 192 111 51 523
Fragments arranged by size:
1011 523 305 192 111 51 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 409 698 2244
Size: 409 289 1546
Fragments arranged by size:
1546 409 289
TatI w'GTAC_w
Cuts at: 0 1703 2048 2126 2162 2244
Size: 1703 345 78 36 82
Fragments arranged by size:
1703 345 82 78 36
Tth111I GACn'n_nGTC
Cuts at: 0 1123 2244
Size: 1123 1121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 65 2244
Size: 65 2179
XmaI C'CCGG_G
Cuts at: 0 1214 2244
Size: 1214 1030
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NcoI NotI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI SanDI SapI ScaI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AleI AlfI
AloI AscI AseI AsiSI AvrII BamHI BbsI BcgI
BclI BfrBI BglII BmtI BspEI BspHI BsrBI BsrDI
BsrGI BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI