Restriction map for
pFN21AE4178
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI BamHI BbsI BbvCI
BclI BfrI BglII BpuAI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MboII MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1389 GACGTC GACGT^C
AccI 763 GTCTAC GT^[AC][GT]AC
AlwI 306 GATCC GGATC(4/5)
AocI 418 CCTCAGG CC^T[ACGT]AGG
AviII 312 TGCGCA TGC^GCA
AvrII 978 CCTAGG C^CTAGG
BcgI 532 CGAGACGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 587 GTATCC GTATCC(6/5)
BlnI 978 CCTAGG C^CTAGG
BsaI 723 GGTCTC GGTCTC(1/5)
BsaWI 1378 ACCGGA [AT]^CCGG[AT]
BsmBI 533 GAGACG CGTCTC(1/5)
BspHI 830 TCATGA T^CATGA
BsrDI 479 GCAATG GCAATG(2/0)
BstXI 575 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 305 AGATCC [GA]^GATC[CT]
Bsu36I 418 CCTCAGG CC^T[ACGT]AGG
DpnII 306 GATC ^GATC
Eco81I 418 CCTCAGG CC^T[ACGT]AGG
FspI 312 TGCGCA TGC^GCA
MboI 306 GATC ^GATC
MflI 305 AGATCC [GA]^GATC[CT]
MslI 880 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 306 GATC ^GATC
PshAI 1389 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 830 TCATGA T^CATGA
Sau3AI 306 GATC ^GATC
SexAI 1712 ACCTGGT A^CC[AT]GGT
SmlI 628 CTCAAG C^T[CT][GA]AG
SphI 251 GCATGC GCATG^C
Tsp509I 1876 AATT ^AATT
XcmI 1802 CCAACAAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 305 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspI 277 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACGCTGTC
BbeI 75 GGCGCC GGCGC^C
1680 GGCGCC
BfaI 728 CTAG C^TAG
979 CTAG
BspMI 623 ACCTGC ACCTGC(4/8)
953 ACCTGC
BsrI 720 ACTGG ACTGG(1/-1)
506 CCAGT
BstAPI 486 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1619 GCACCAGCTGC
EagI 23 CGGCCG C^GGCCG
1319 CGGCCG
EclXI 23 CGGCCG C^GGCCG
1319 CGGCCG
Eco52I 23 CGGCCG C^GGCCG
1319 CGGCCG
KasI 75 GGCGCC G^GCGCC
1680 GGCGCC
MaeI 728 CTAG C^TAG
979 CTAG
NarI 75 GGCGCC GG^CGCC
1680 GGCGCC
PflFI 277 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACGCTGTC
PpuMI 923 AGGACCT [GA]G^G[AT]CC[CT]
1195 GGGACCT
SfoI 75 GGCGCC GGC^GCC
1680 GGCGCC
StuI 422 AGGCCT AGG^CCT
1079 AGGCCT
Tsp45I 397 GTGAC ^GT[GC]AC
1234 GTGAC
Tth111I 277 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1899 GACGCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PstI
PvuII RsaI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
TspRI TthHB8I Van91I XmaCI XmaI