Restriction map for
pFN21AE4178
(Linear) (Six-base) MAPSORT of: KIEE4178.seq Check: 4149 from: 1 to: 1926
KIEE4178.orf
With 182 enzymes: SgfI *
March 22, 2012 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 962 1926
Size: 962 964
AatII G_ACGT'C
Cuts at: 0 1393 1926
Size: 1393 533
AccI GT'mk_AC
Cuts at: 0 764 1926
Size: 764 1162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 964 1926
Size: 734 230 962
Fragments arranged by size:
962 734 230
AflIII A'CryG_T
Cuts at: 0 994 1241 1523 1926
Size: 994 247 282 403
Fragments arranged by size:
994 403 282 247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 311 640 1926
Size: 311 329 1286
Fragments arranged by size:
1286 329 311
AlwNI CAG_nnn'CTG
Cuts at: 0 177 492 714 1926
Size: 177 315 222 1212
Fragments arranged by size:
1212 315 222 177
ApaI G_GGCC'C
Cuts at: 0 65 71 1140 1926
Size: 65 6 1069 786
Fragments arranged by size:
1069 786 65 6
AvaI C'yCGr_G
Cuts at: 0 57 64 70 109 1139 1794 1926
Size: 57 7 6 39 1030 655 132
Fragments arranged by size:
1030 655 132 57 39 7 6
AvrII C'CTAG_G
Cuts at: 0 978 1926
Size: 978 948
BanI G'GyrC_C
Cuts at: 0 75 905 982 1588 1618 1680 1926
Size: 75 830 77 606 30 62 246
Fragments arranged by size:
830 606 246 77 75 62 30
BanII G_rGCy'C
Cuts at: 0 43 65 71 1140 1514 1926
Size: 43 22 6 1069 374 412
Fragments arranged by size:
1069 412 374 43 22 6
BbeI G_GCGC'C
Cuts at: 0 79 1684 1926
Size: 79 1605 242
Fragments arranged by size:
1605 242 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 555 1926
Size: 521 34 1371
Fragments arranged by size:
1371 521 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 598 1926
Size: 598 1328
BglI GCCn_nnn'nGGC
Cuts at: 0 26 112 188 238 263 454 1926
Size: 26 86 76 50 25 191 1472
Fragments arranged by size:
1472 191 86 76 50 26 25
BlpI GC'TnA_GC
Cuts at: 0 178 856 1549 1563 1926
Size: 178 678 693 14 363
Fragments arranged by size:
693 678 363 178 14
Bme1580I G_kGCm'C
Cuts at: 0 65 71 1140 1270 1593 1926
Size: 65 6 1069 130 323 333
Fragments arranged by size:
1069 333 323 130 65 6
BmgBI CAC'GTC
Cuts at: 0 669 798 997 1926
Size: 669 129 199 929
Fragments arranged by size:
929 669 199 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 1682 1749 1885 1926
Size: 1603 79 67 136 41
Fragments arranged by size:
1603 136 79 67 41
Bpu10I CC'TnA_GC
Cuts at: 0 778 962 1926
Size: 778 184 964
Fragments arranged by size:
964 778 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1926
Size: 613 1313
BsaI GGTCTCn'nnnn_
Cuts at: 0 729 1926
Size: 729 1197
BsaAI yAC'GTr
Cuts at: 0 291 396 648 882 1110 1244 1599 1926
Size: 291 105 252 234 228 134 355 327
Fragments arranged by size:
355 327 291 252 234 228 134 105
BsaHI Gr'CG_yC
Cuts at: 0 76 527 803 974 1390 1540 1681 1926
Size: 76 451 276 171 416 150 141 245
Fragments arranged by size:
451 416 276 245 171 150 141 76
BsaWI w'CCGG_w
Cuts at: 0 1378 1926
Size: 1378 548
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1324 1354 1462 1492 1747 1777 1926
Size: 1324 30 108 30 255 30 149
Fragments arranged by size:
1324 255 149 108 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 769 772 919 1470 1926
Size: 697 72 3 147 551 456
Fragments arranged by size:
697 551 456 147 72 3
BseYI C'CCAG_C
Cuts at: 0 498 782 873 1926
Size: 498 284 91 1053
Fragments arranged by size:
1053 498 284 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 271 388 460 505 1926
Size: 190 81 117 72 45 1421
Fragments arranged by size:
1421 190 117 81 72 45
BsiEI CG_ry'CG
Cuts at: 0 26 1101 1322 1926
Size: 26 1075 221 604
Fragments arranged by size:
1075 604 221 26
BsiHKAI G_wGCw'C
Cuts at: 0 50 1411 1705 1926
Size: 50 1361 294 221
Fragments arranged by size:
1361 294 221 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 527 1926
Size: 527 1399
Bsp1286I G_dGCh'C
Cuts at: 0 43 50 65 71 1140 1270 1411 1514
Size: 43 7 15 6 1069 130 141 103
Cuts at: 1514 1593 1705 1926
Size: 79 112 221
Fragments arranged by size:
1069 221 141 130 112 103 79 43
15 7 6
BspHI T'CATG_A
Cuts at: 0 830 1926
Size: 830 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 632 962 1926
Size: 632 330 964
Fragments arranged by size:
964 632 330
BsrDI GCAATG_nn'
Cuts at: 0 486 1926
Size: 486 1440
BsrFI r'CCGG_y
Cuts at: 0 25 102 1166 1321 1926
Size: 25 77 1064 155 605
Fragments arranged by size:
1064 605 155 77 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 1625 1926
Size: 492 1133 301
Fragments arranged by size:
1133 492 301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 1926
Size: 582 1344
BstYI r'GATC_y
Cuts at: 0 305 1926
Size: 305 1621
Bsu36I CC'TnA_GG
Cuts at: 0 419 1926
Size: 419 1507
BtgI C'CryG_G
Cuts at: 0 155 823 1065 1214 1262 1535 1721 1742
Size: 155 668 242 149 48 273 186 21
Cuts at: 1742 1926
Size: 184
Fragments arranged by size:
668 273 242 186 184 155 149 48
21
BtsI GCAGTG_nn'
Cuts at: 0 1851 1926
Size: 1851 75
DraIII CAC_nnn'GTG
Cuts at: 0 198 321 663 798 1113 1926
Size: 198 123 342 135 315 813
Fragments arranged by size:
813 342 315 198 135 123
EaeI y'GGCC_r
Cuts at: 0 23 239 671 1292 1319 1433 1490 1574
Size: 23 216 432 621 27 114 57 84
Cuts at: 1574 1718 1814 1926
Size: 144 96 112
Fragments arranged by size:
621 432 216 144 114 112 96 84
57 27 23
EagI C'GGCC_G
Cuts at: 0 23 1319 1926
Size: 23 1296 607
Fragments arranged by size:
1296 607 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 964 1603 1682 1749 1885 1926
Size: 734 230 639 79 67 136 41
Fragments arranged by size:
734 639 230 136 79 67 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 706 934 1926
Size: 628 78 228 992
Fragments arranged by size:
992 628 228 78
EcoO109I rG'GnC_Cy
Cuts at: 0 61 924 939 1196 1337 1926
Size: 61 863 15 257 141 589
Fragments arranged by size:
863 589 257 141 61 15
FspI TGC'GCA
Cuts at: 0 314 1926
Size: 314 1612
FspAI rTGC'GCAy
Cuts at: 0 314 1926
Size: 314 1612
HaeII r_GCGC'y
Cuts at: 0 21 79 1008 1422 1684 1926
Size: 21 58 929 414 262 242
Fragments arranged by size:
929 414 262 242 58 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1183 1215 1324 1356 1465 1497
Size: 595 32 556 32 109 32 109 32
Cuts at: 1497 1635 1667 1747 1779 1926
Size: 138 32 80 32 147
Fragments arranged by size:
595 556 147 138 109 109 80 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 75 1680 1926
Size: 75 1605 246
Fragments arranged by size:
1605 246 75
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1926
Size: 599 1327
MscI TGG'CCA
Cuts at: 0 241 673 1435 1492 1720 1926
Size: 241 432 762 57 228 206
Fragments arranged by size:
762 432 241 228 206 57
MslI CAynn'nnrTG
Cuts at: 0 884 1926
Size: 884 1042
MspA1I CmG'CkG
Cuts at: 0 174 435 519 1103 1567 1625 1926
Size: 174 261 84 584 464 58 301
Fragments arranged by size:
584 464 301 261 174 84 58
NaeI GCC'GGC
Cuts at: 0 27 104 1323 1926
Size: 27 77 1219 603
Fragments arranged by size:
1219 603 77 27
NarI GG'CG_CC
Cuts at: 0 76 1681 1926
Size: 76 1605 245
Fragments arranged by size:
1605 245 76
NcoI C'CATG_G
Cuts at: 0 155 1262 1721 1926
Size: 155 1107 459 205
Fragments arranged by size:
1107 459 205 155
NgoMIV G'CCGG_C
Cuts at: 0 25 102 1321 1926
Size: 25 77 1219 605
Fragments arranged by size:
1219 605 77 25
NspI r_CATG'y
Cuts at: 0 255 820 1830 1926
Size: 255 565 1010 96
Fragments arranged by size:
1010 565 255 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 1660 1808 1926
Size: 198 1462 148 118
Fragments arranged by size:
1462 198 148 118
PmlI CAC'GTG
Cuts at: 0 291 396 882 1110 1244 1926
Size: 291 105 486 228 134 682
Fragments arranged by size:
682 486 291 228 134 105
PpuMI rG'GwC_Cy
Cuts at: 0 924 1196 1926
Size: 924 272 730
Fragments arranged by size:
924 730 272
PshAI GACnn'nnGTC
Cuts at: 0 1393 1926
Size: 1393 533
PspOMI G'GGCC_C
Cuts at: 0 61 67 1136 1926
Size: 61 6 1069 790
Fragments arranged by size:
1069 790 61 6
PstI C_TGCA'G
Cuts at: 0 340 518 619 656 1926
Size: 340 178 101 37 1270
Fragments arranged by size:
1270 340 178 101 37
PvuII CAG'CTG
Cuts at: 0 174 435 519 1625 1926
Size: 174 261 84 1106 301
Fragments arranged by size:
1106 301 261 174 84
SexAI A'CCwGG_T
Cuts at: 0 1712 1926
Size: 1712 214
SfcI C'TryA_G
Cuts at: 0 336 514 615 652 1926
Size: 336 178 101 37 1274
Fragments arranged by size:
1274 336 178 101 37
SfoI GGC'GCC
Cuts at: 0 77 1682 1926
Size: 77 1605 244
Fragments arranged by size:
1605 244 77
SmaI CCC'GGG
Cuts at: 0 59 66 1141 1926
Size: 59 7 1075 785
Fragments arranged by size:
1075 785 59 7
SmlI C'TyrA_G
Cuts at: 0 628 1926
Size: 628 1298
SphI G_CATG'C
Cuts at: 0 255 1926
Size: 255 1671
SrfI GCCC'GGGC
Cuts at: 0 66 1926
Size: 66 1860
StuI AGG'CCT
Cuts at: 0 424 1081 1926
Size: 424 657 845
Fragments arranged by size:
845 657 424
StyI C'CwwG_G
Cuts at: 0 155 299 425 978 1262 1721 1926
Size: 155 144 126 553 284 459 205
Fragments arranged by size:
553 459 284 205 155 144 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 774 1276 1800 1840 1926
Size: 694 80 502 524 40 86
Fragments arranged by size:
694 524 502 86 80 40
TatI w'GTAC_w
Cuts at: 0 1313 1787 1926
Size: 1313 474 139
Fragments arranged by size:
1313 474 139
Tth111I GACn'n_nGTC
Cuts at: 0 280 1902 1926
Size: 280 1622 24
Fragments arranged by size:
1622 280 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1809 1926
Size: 1809 117
XmaI C'CCGG_G
Cuts at: 0 57 64 1139 1926
Size: 57 7 1075 787
Fragments arranged by size:
1075 787 57 7
ZraI GAC'GTC
Cuts at: 0 1391 1926
Size: 1391 535
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApaI
AvaI AvrII BanI BanII BbeI BcgI BciVI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PmlI PpuMI PshAI PspOMI PstI PvuII SexAI
SfcI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI
BbvCI BclI BfrBI BglII BmrI BmtI BplI BsaBI
BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SspI SwaI TstI XbaI XhoI XmnI