Restriction map for
pFN21AE1161
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI Alw44I AlwI
AocI ApaI ApaLI AscI Asp718I
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BglI BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DsaI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MluI MroI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AclI 349 AACGTT AA^CGTT
AflII 1059 CTTAAG C^TTAAG
AhdI 553 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 967 AGCGCT AGC^GCT
AseI 690 ATTAAT AT^TAAT
AsnI 690 ATTAAT AT^TAAT
AspEI 553 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1560 GAGCTC G[AT]GC[AT]^C
AspI 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 653 TGGCCA TGG^CCA
BanI 1655 GGTGCC G^G[CT][GA]CC
BanII 1560 GAGCTC G[GA]GC[CT]^C
BbsI 520 GTCTTC GAAGAC(2/6)
BbvCI 1545 GCTGAGG CCTCAGC(-5/-2)
BclI 1644 TGATCA T^GATCA
BcnI 549 CCGGG CC^[GC]GG
BfrI 1059 CTTAAG C^TTAAG
BlpI 1562 GCTCAGC GC^T[ACGT]AGC
BpmI 1557 CTGGAG CTGGAG(16/14)
Bpu1102I 1562 GCTCAGC GC^T[ACGT]AGC
BpuAI 520 GTCTTC GAAGAC(2/6)
BseRI 544 CTCCTC GAGGAG(10/8)
BsiHKAI 1560 GAGCTC G[AT]GC[AT]^C
Bsp1407I 150 TGTACA T^GTACA
BspHI 131 TCATGA T^CATGA
BsrGI 150 TGTACA T^GTACA
CelII 1562 GCTCAGC GC^T[ACGT]AGC
DraI 1135 TTTAAA TTT^AAA
DrdI 511 GACACGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 653 TGGCCA [CT]^GGCC[GA]
Eam1105I 553 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 967 AGCGCT AGC^GCT
EcoT14I 1569 CCAAGG C^C[AT][AT]GG
EcoT22I 727 ATGCAT ATGCA^T
FbaI 1644 TGATCA T^GATCA
HaeII 967 AGCGCT [GA]GCGC^[CT]
HapII 549 CCGG C^CGG
HincII 34 GTTGAC GT[CT]^[GA]AC
HindII 34 GTTGAC GT[CT]^[GA]AC
HpaII 549 CCGG C^CGG
MfeI 1460 CAATTG C^AATTG
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
MslI 855 CATTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1007 CAGCTG C[AC]G^C[GT]G
MspI 549 CCGG C^CGG
MunI 1460 CAATTG C^AATTG
NciI 549 CCGGG CC^[GC]GG
NsiI 727 ATGCAT ATGCA^T
PflFI 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 551 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 690 ATTAAT AT^TAAT
Psp1406I 349 AACGTT AA^CGTT
PstI 974 CTGCAG CTGCA^G
PvuII 1007 CAGCTG CAG^CTG
RcaI 131 TCATGA T^CATGA
SacI 1560 GAGCTC GAGCT^C
SfcI 974 CTGCAG C^T[GA][CT]AG
SphI 1366 GCATGC GCATG^C
SspBI 150 TGTACA T^GTACA
StuI 1377 AGGCCT AGG^CCT
StyI 1569 CCAAGG C^C[AT][AT]GG
TaqI 907 TCGA T^CGA
Tth111I 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 907 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 324 CCGC CCGC(-3/-1)
1028 GCGG
AcsI 215 GAATTT [GA]^AATT[CT]
405 AAATTC
AlwNI 293 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
638 CAGTTACTG
ApoI 215 GAATTT [GA]^AATT[CT]
405 AAATTC
Asp700I 215 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAGAACTTC
BfaI 902 CTAG C^TAG
1180 CTAG
BglII 250 AGATCT A^GATCT
1553 AGATCT
BmyI 1560 GAGCTC G[AGT]GC[ACT]^C
1656 GTGCCC
BsaJI 8 CCCAGG C^C[ACGT][ACGT]GG
1569 CCAAGG
BsgI 572 GTGCAG GTGCAG(16/14)
1004 GTGCAG
BsmFI 551 GGGAC GGGAC(10/14)
715 GGGAC
Bsp1286I 1560 GAGCTC G[AGT]GC[ACT]^C
1656 GTGCCC
BssSI 991 CTCGTG CACGAG(-5/-1)
1210 CTCGTG
BstXI 1298 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAAAGGCCTGG
BstYI 250 AGATCT [GA]^GATC[CT]
1553 AGATCT
CfoI 968 GCGC GCG^C
1370 GCGC
DraII 551 GGGACCC [GA]G^G[ACGT]CC[CT]
1481 AGGCCCT
EcoO109I 551 GGGACCC [GA]G^G[ACGT]CC[CT]
1481 AGGCCCT
HhaI 968 GCGC GCG^C
1370 GCGC
HinP1I 968 GCGC G^CGC
1370 GCGC
MaeI 902 CTAG C^TAG
1180 CTAG
MaeII 350 ACGT A^CGT
564 ACGT
MflI 250 AGATCT [GA]^GATC[CT]
1553 AGATCT
NspI 725 ACATGC [GA]CATG^[CT]
1366 GCATGC
PleI 600 GACTC GAGTC(4/5)
1266 GAGTC
SmlI 475 CTTGAG C^T[CT][GA]AG
1059 CTTAAG
SspI 766 AATATT AAT^ATT
1498 AATATT
XhoII 250 AGATCT [GA]^GATC[CT]
1553 AGATCT
XmnI 215 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI