Restriction map for
pFN21AE1161
(Circular) (Six-base) MAPSORT of: pFN21AE1161.seq Check: 6684 from: 1 to: 6367
pFN21AE1161 6367 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4177 278
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3710 1937
Size: 1773 4594
Acc65I G'GTAC_C
Cuts at: 3688 3688
Size: 6367
AclI AA'CG_TT
Cuts at: 1563 2355 4494 4867 1563
Size: 792 2139 373 3063
Fragments arranged by size:
3063 2139 792 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4430 5814 6082 1632
Size: 2798 1384 268 1917
Fragments arranged by size:
2798 1917 1384 268
AfeI AGC'GCT
Cuts at: 2974 2974
Size: 6367
AflII C'TTAA_G
Cuts at: 820 1017 3064 820
Size: 197 2047 4123
Fragments arranged by size:
4123 2047 197
AflIII A'CryG_T
Cuts at: 1203 5266 6243 1203
Size: 4063 977 1327
Fragments arranged by size:
4063 1327 977
AgeI A'CCGG_T
Cuts at: 5198 5198
Size: 6367
AhdI GACnn_n'nnGTC
Cuts at: 2563 5096 2563
Size: 2533 3834
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3014 1184
Size: 1830 4537
AlwNI CAG_nnn'CTG
Cuts at: 2303 2648 4110 5682 2303
Size: 345 1462 1572 2988
Fragments arranged by size:
2988 1572 1462 345
ApaLI G'TGCA_C
Cuts at: 4424 5580 4424
Size: 1156 5211
ApoI r'AATT_y
Cuts at: 1493 2220 2410 3676 3902 4054 6163 1493
Size: 727 190 1266 226 152 2109 1697
Fragments arranged by size:
2109 1697 1266 727 226 190 152
AseI AT'TA_AT
Cuts at: 160 1032 2696 4921 160
Size: 872 1664 2225 1606
Fragments arranged by size:
2225 1664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
AvaI C'yCGr_G
Cuts at: 1870 1943 3692 1870
Size: 73 1749 4545
Fragments arranged by size:
4545 1749 73
BamHI G'GATC_C
Cuts at: 3697 4064 3697
Size: 367 6000
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3660 3688 5143 618
Size: 325 217 627 1873 28 1455 1842
Fragments arranged by size:
1873 1842 1455 627 325 217 28
BanII G_rGCy'C
Cuts at: 1391 3569 3686 1391
Size: 2178 117 4072
Fragments arranged by size:
4072 2178 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2518 928
Size: 971 619 4777
Fragments arranged by size:
4777 971 619
BbvCI CC'TCA_GC
Cuts at: 3551 3551
Size: 6367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4558 4592 1903
Size: 34 2621 34 3678
Fragments arranged by size:
3678 2621 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4259 5469 4259
Size: 1210 5157
BclI T'GATC_A
Cuts at: 1548 3649 1548
Size: 2101 4266
BfrBI ATG'CAT
Cuts at: 2734 2734
Size: 6367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4978 136
Size: 107 122 71 631 605 3306 1525
Fragments arranged by size:
3306 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2255 3558 6362 2255
Size: 1303 2804 2260
Fragments arranged by size:
2804 2260 1303
BlpI GC'TnA_GC
Cuts at: 3568 3784 3568
Size: 216 6151
Bme1580I G_kGCm'C
Cuts at: 1790 3665 4428 5584 1790
Size: 1875 763 1156 2573
Fragments arranged by size:
2573 1875 1156 763
BmgBI CAC'GTC
Cuts at: 1318 2570 1318
Size: 1252 5115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4098 5056 442
Size: 416 53 83 399 24 2681 958 1753
Fragments arranged by size:
2681 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 6367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3583 5027 1239
Size: 2344 1444 2579
Fragments arranged by size:
2579 2344 1444
Bpu10I CC'TnA_GC
Cuts at: 3551 3551
Size: 6367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 4492 5357 5655 2501
Size: 1991 865 298 3213
Fragments arranged by size:
3213 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 2784 3394 5030 882
Size: 617 918 367 610 1636 2219
Fragments arranged by size:
2219 1636 918 617 610 367
BsaAI yAC'GTr
Cuts at: 493 1204 4158 493
Size: 711 2954 2702
Fragments arranged by size:
2954 2702 711
BsaBI GATnn'nnATC
Cuts at: 6361 6361
Size: 6367
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4174 4556 5191 275
Size: 53 83 186 936 2641 382 635 1451
Fragments arranged by size:
2641 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4800 5198 5472 5619 6030 4800
Size: 398 274 147 411 5137
Fragments arranged by size:
5137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6142
Fragments arranged by size:
6142 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 1186
Size: 1354 5013
BseYI C'CCAG_C
Cuts at: 3344 5570 6066 3344
Size: 2226 496 3645
Fragments arranged by size:
3645 2226 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2598 3030 1163
Size: 573 862 432 4500
Fragments arranged by size:
4500 862 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3817 4578 5606 1540
Size: 275 186 1816 761 1028 2301
Fragments arranged by size:
2301 1816 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3569 4428 4513 5584 3569
Size: 859 85 1071 4352
Fragments arranged by size:
4352 1071 859 85
BsmI GAATG_Cn'
Cuts at: 3878 3971 3878
Size: 93 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3569 3665 3686 4428 4513 5584 1391
Size: 399 1779 96 21 742 85 1071 2174
Fragments arranged by size:
2174 1779 1071 742 399 96 85 21
BspEI T'CCGG_A
Cuts at: 6030 6030
Size: 6367
BspHI T'CATG_A
Cuts at: 2136 4256 5986 2136
Size: 2120 1730 2517
Fragments arranged by size:
2517 2120 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3722 844
Size: 2878 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4254 1394
Size: 429 2431 3507
Fragments arranged by size:
3507 2431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4862 5036 65
Size: 1183 3614 174 1396
Fragments arranged by size:
3614 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5011 5198 1700
Size: 110 3201 187 2869
Fragments arranged by size:
3201 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2155 96
Size: 2059 4308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6367
BssSI C'ACGA_G
Cuts at: 2996 3215 4427 5439 2996
Size: 219 1212 1012 3924
Fragments arranged by size:
3924 1212 1012 219
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6367
BstBI TT'CG_AA
Cuts at: 5171 5171
Size: 6367
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6367
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3385 3310
Size: 75 6292
BstYI r'GATC_y
Cuts at: 1972 2255 3558 3697 4064 4449 4466 5918 6004
Size: 283 1303 139 367 385 17 1452 86
Cuts at: 6004 6016 6362 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1303 385 367 346 283 139
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4876
Fragments arranged by size:
4876 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5628
Fragments arranged by size:
5628 683 56
BtsI GCAGTG_nn'
Cuts at: 3883 4677 4697 3883
Size: 794 20 5553
Fragments arranged by size:
5553 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6332
DraI TTT'AAA
Cuts at: 3142 3671 3142
Size: 529 5838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 5374 809
Size: 507 1206 2852 1802
Fragments arranged by size:
2852 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2658 3814 4703
Size: 54 1425 50 275 18 828 1156 889
Cuts at: 4703 6332 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1156 889 828 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3814 1537
Size: 275 2002 4090
Fragments arranged by size:
4090 2002 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4297 1353
Size: 2944 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4954 5328 5474 387
Size: 1064 138 3365 374 146 1280
Fragments arranged by size:
3365 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3567 3567
Size: 6367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3583 4430 5027 5814 6082 1239
Size: 393 1951 847 597 787 268 1524
Fragments arranged by size:
1951 1524 847 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2557 3487 2557
Size: 930 5437
EcoRI G'AATT_C
Cuts at: 3676 4054 3676
Size: 378 5989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2265 2833 2265
Size: 568 5799
FspI TGC'GCA
Cuts at: 4873 4873
Size: 6367
HaeII r_GCGC'y
Cuts at: 1678 2976 5514 1678
Size: 1298 2538 2531
Fragments arranged by size:
2538 2531 1298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 5008 5040 5082
Size: 32 135 32 977 32 2427 32 42
Cuts at: 5082 5114 1373
Size: 32 2626
Fragments arranged by size:
2626 2427 977 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 3711 3957 669
Size: 988 281 103 1670 246 3079
Fragments arranged by size:
3079 1670 988 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6367
HpaI GTT'AAC
Cuts at: 3957 3957
Size: 6367
KpnI G_GTAC'C
Cuts at: 3692 3692
Size: 6367
MfeI C'AATT_G
Cuts at: 3465 3966 3465
Size: 501 5866
MluI A'CGCG_T
Cuts at: 6243 6243
Size: 6367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 3492 3498 5481 5665 1305
Size: 1104 1083 6 1983 184 2007
Fragments arranged by size:
2007 1983 1104 1083 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 3717
Fragments arranged by size:
3717 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 4325 4684 4843 518
Size: 648 1698 1461 359 159 2042
Fragments arranged by size:
2042 1698 1461 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3014 3783 4460 5608 5853 1606
Size: 1408 769 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 769 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4876
Fragments arranged by size:
4876 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6367
NotI GC'GGCC_GC
Cuts at: 3814 3814
Size: 6367
NsiI A_TGCA'T
Cuts at: 2736 2736
Size: 6367
NspI r_CATG'y
Cuts at: 1132 1229 2734 3375 3725 5270 1132
Size: 97 1505 641 350 1545 2229
Fragments arranged by size:
2229 1545 1505 641 350 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6367
PciI A'CATG_T
Cuts at: 5266 5266
Size: 6367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6367
PfoI T'CCnGG_A
Cuts at: 2553 2553
Size: 6367
PmeI GTTT'AAAC
Cuts at: 3671 3671
Size: 6367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 5949 5981 4436
Size: 32 1481 32 4822
Fragments arranged by size:
4822 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 2557
Size: 6367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6367
PsiI TTA'TAA
Cuts at: 3937 3937
Size: 6367
PstI C_TGCA'G
Cuts at: 830 2983 3719 830
Size: 2153 736 3478
Fragments arranged by size:
3478 2153 736
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6367
PvuII CAG'CTG
Cuts at: 3014 3014
Size: 6367
SacI G_AGCT'C
Cuts at: 3569 3569
Size: 6367
SalI G'TCGA_C
Cuts at: 1936 3709 1936
Size: 1773 4594
SanDI GG'GwC_CC
Cuts at: 2557 2557
Size: 6367
SbfI CC_TGCA'GG
Cuts at: 3719 3719
Size: 6367
ScaI AGT'ACT
Cuts at: 4615 6327 4615
Size: 1712 4655
SfcI C'TryA_G
Cuts at: 826 1045 2979 3715 4850 5531 5722 826
Size: 219 1934 736 1135 681 191 1471
Fragments arranged by size:
1934 1471 1135 736 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6367
SmaI CCC'GGG
Cuts at: 1872 3694 1872
Size: 1822 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 3064 4471 5372 5634 820
Size: 197 926 537 584 1407 901 262 1553
Fragments arranged by size:
1553 1407 926 901 584 537 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 493
Size: 3665 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6367
SphI G_CATG'C
Cuts at: 1132 3375 3725 1132
Size: 2243 350 3774
Fragments arranged by size:
3774 2243 350
SspI AAT'ATT
Cuts at: 5 52 2773 3505 4291 5
Size: 47 2721 732 786 2081
Fragments arranged by size:
2721 2081 786 732 47
StuI AGG'CCT
Cuts at: 1517 3384 1517
Size: 1867 4500
StyI C'CwwG_G
Cuts at: 513 1065 2004 3574 3806 513
Size: 552 939 1570 232 3074
Fragments arranged by size:
3074 1570 939 552 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2631 2903 3434 4394 4411 4564 4749
Size: 1255 1051 272 531 960 17 153 185
Cuts at: 4749 5194 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1051 960 531 445 272 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 3469 4613
Size: 274 80 33 51 1443 178 1314 1144
Cuts at: 4613 6325 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1314 1144 274 178 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 6327 6359 2303
Size: 32 3992 32 2311
Fragments arranged by size:
3992 2311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3270 1316
Size: 1954 4413
XbaI T'CTAG_A
Cuts at: 3703 3703
Size: 6367
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6367
XmaI C'CCGG_G
Cuts at: 1870 3692 1870
Size: 1822 4545
XmnI GAAnn'nnTTC
Cuts at: 2224 2248 4496 2224
Size: 24 2248 4095
Fragments arranged by size:
4095 2248 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4175 276
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI