Restriction map for
pFN21AE1161
(Linear) (Six-base) MAPSORT of: KIEE1161.seq Check: 7054 from: 1 to: 1662
KIEE1161.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AclI AA'CG_TT
Cuts at: 0 350 1662
Size: 350 1312
AfeI AGC'GCT
Cuts at: 0 969 1662
Size: 969 693
AflII C'TTAA_G
Cuts at: 0 1059 1662
Size: 1059 603
AhdI GACnn_n'nnGTC
Cuts at: 0 558 1662
Size: 558 1104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1009 1662
Size: 1009 653
AlwNI CAG_nnn'CTG
Cuts at: 0 298 643 1662
Size: 298 345 1019
Fragments arranged by size:
1019 345 298
ApoI r'AATT_y
Cuts at: 0 215 405 1662
Size: 215 190 1257
Fragments arranged by size:
1257 215 190
AseI AT'TA_AT
Cuts at: 0 691 1662
Size: 691 971
BanI G'GyrC_C
Cuts at: 0 1655 1662
Size: 1655 7
BanII G_rGCy'C
Cuts at: 0 1564 1662
Size: 1564 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 513 1662
Size: 513 1149
BbvCI CC'TCA_GC
Cuts at: 0 1546 1662
Size: 1546 116
BclI T'GATC_A
Cuts at: 0 1644 1662
Size: 1644 18
BfrBI ATG'CAT
Cuts at: 0 729 1662
Size: 729 933
BglII A'GATC_T
Cuts at: 0 250 1553 1662
Size: 250 1303 109
Fragments arranged by size:
1303 250 109
BlpI GC'TnA_GC
Cuts at: 0 1563 1662
Size: 1563 99
Bme1580I G_kGCm'C
Cuts at: 0 1660 1662
Size: 1660 2
BmgBI CAC'GTC
Cuts at: 0 565 1662
Size: 565 1097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1464 1662
Size: 1464 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1578 1662
Size: 1578 84
Bpu10I CC'TnA_GC
Cuts at: 0 1546 1662
Size: 1546 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1662
Size: 496 1166
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 779 1389 1662
Size: 412 367 610 273
Fragments arranged by size:
610 412 367 273
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 1662
Size: 535 1127
BseYI C'CCAG_C
Cuts at: 0 1339 1662
Size: 1339 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1025 1662
Size: 593 432 637
Fragments arranged by size:
637 593 432
BsiHKAI G_wGCw'C
Cuts at: 0 1564 1662
Size: 1564 98
Bsp1286I G_dGCh'C
Cuts at: 0 1564 1660 1662
Size: 1564 96 2
Fragments arranged by size:
1564 96 2
BspHI T'CATG_A
Cuts at: 0 131 1662
Size: 131 1531
BsrGI T'GTAC_A
Cuts at: 0 150 1662
Size: 150 1512
BssSI C'ACGA_G
Cuts at: 0 991 1210 1662
Size: 991 219 452
Fragments arranged by size:
991 452 219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 1380 1662
Size: 1305 75 282
Fragments arranged by size:
1305 282 75
BstYI r'GATC_y
Cuts at: 0 250 1553 1662
Size: 250 1303 109
Fragments arranged by size:
1303 250 109
DraI TTT'AAA
Cuts at: 0 1137 1662
Size: 1137 525
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 1662
Size: 517 1145
EaeI y'GGCC_r
Cuts at: 0 653 1662
Size: 653 1009
EcoICRI GAG'CTC
Cuts at: 0 1562 1662
Size: 1562 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1578 1662
Size: 1578 84
EcoO109I rG'GnC_Cy
Cuts at: 0 552 1482 1662
Size: 552 930 180
Fragments arranged by size:
930 552 180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 260 828 1662
Size: 260 568 834
Fragments arranged by size:
834 568 260
HaeII r_GCGC'y
Cuts at: 0 971 1662
Size: 971 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 1662
Size: 544 32 1086
Fragments arranged by size:
1086 544 32
HincII GTy'rAC
Cuts at: 0 36 1662
Size: 36 1626
MfeI C'AATT_G
Cuts at: 0 1460 1662
Size: 1460 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1487 1493 1662
Size: 404 1083 6 169
Fragments arranged by size:
1083 404 169 6
MscI TGG'CCA
Cuts at: 0 655 1662
Size: 655 1007
MslI CAynn'nnrTG
Cuts at: 0 859 1662
Size: 859 803
MspA1I CmG'CkG
Cuts at: 0 1009 1662
Size: 1009 653
NsiI A_TGCA'T
Cuts at: 0 731 1662
Size: 731 931
NspI r_CATG'y
Cuts at: 0 729 1370 1662
Size: 729 641 292
Fragments arranged by size:
729 641 292
PfoI T'CCnGG_A
Cuts at: 0 548 1662
Size: 548 1114
PpuMI rG'GwC_Cy
Cuts at: 0 552 1662
Size: 552 1110
PstI C_TGCA'G
Cuts at: 0 978 1662
Size: 978 684
PvuII CAG'CTG
Cuts at: 0 1009 1662
Size: 1009 653
SacI G_AGCT'C
Cuts at: 0 1564 1662
Size: 1564 98
SanDI GG'GwC_CC
Cuts at: 0 552 1662
Size: 552 1110
SfcI C'TryA_G
Cuts at: 0 974 1662
Size: 974 688
SmlI C'TyrA_G
Cuts at: 0 475 1059 1662
Size: 475 584 603
Fragments arranged by size:
603 584 475
SphI G_CATG'C
Cuts at: 0 1370 1662
Size: 1370 292
SspI AAT'ATT
Cuts at: 0 768 1500 1662
Size: 768 732 162
Fragments arranged by size:
768 732 162
StuI AGG'CCT
Cuts at: 0 1379 1662
Size: 1379 283
StyI C'CwwG_G
Cuts at: 0 1569 1662
Size: 1569 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 626 898 1429 1662
Size: 626 272 531 233
Fragments arranged by size:
626 531 272 233
TatI w'GTAC_w
Cuts at: 0 150 1464 1662
Size: 150 1314 198
Fragments arranged by size:
1314 198 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 1662
Size: 298 32 1332
Fragments arranged by size:
1332 298 32
Tth111I GACn'n_nGTC
Cuts at: 0 1265 1662
Size: 1265 397
XmnI GAAnn'nnTTC
Cuts at: 0 219 243 1662
Size: 219 24 1419
Fragments arranged by size:
1419 219 24
Enzymes that do cut:
AclI AfeI AflII AhdI AlfI AlwNI ApoI AseI
BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrGI BssSI BstXI
BstYI DraI DrdI EaeI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I
NsiI NspI PfoI PpuMI PstI PvuII SacI SanDI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BciVI BglI BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII EagI EarI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI XbaI XcmI XhoI XmaI
ZraI