################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 800 CGCG CG^CG AflIII 617 ACGTGT A^C[GA][CT]GT AvaI 640 CCCGGG C^[CT]CG[GA]G AvaII 844 GGACC G^G[AT]CC BamHI 269 GGATCC G^GATCC BfaI 298 CTAG C^TAG BsgI 646 GTGCAG GTGCAG(16/14) BsiEI 287 CGGTCG CG[GA][CT]^CG BsmBI 396 CGTCTC CGTCTC(1/5) BsmFI 843 GGGAC GGGAC(10/14) BsoBI 640 CCCGGG C^[CT]CG[GA]G BsrBI 740 GAGCGG CCGCTC(-3/-3) BstAPI 862 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 672 GGTCACC G^GT[ACGT]ACC BstPI 672 GGTCACC G^GT[ACGT]ACC BstUI 800 CGCG CG^CG BstXI 339 CCAGGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 588 CCACGG C^C[GA][CT]GG EcoNI 443 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 672 GGTCACC G^GT[ACGT]ACC HaeII 1045 AGCGCC [GA]GCGC^[CT] HincII 1021 GTTGAC GT[CT]^[GA]AC HindII 1021 GTTGAC GT[CT]^[GA]AC MaeI 298 CTAG C^TAG MaeII 617 ACGT A^CGT MslI 804 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 800 CGCG CG^CG NaeI 918 GCCGGC GCC^GGC NgoMIV 918 GCCGGC G^CCGGC NspI 862 GCATGC [GA]CATG^[CT] PflMI 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 763 GAGTC GAGTC(4/5) PpuMI 843 GGGACCC [GA]G^G[AT]CC[CT] SexAI 338 ACCAGGT A^CC[AT]GGT SmaI 640 CCCGGG CCC^GGG SphI 862 GCATGC GCATG^C TfiI 769 GAATC G^A[AT]TC Tsp509I 130 AATT ^AATT Van91I 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 734 CCATCCGAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 640 CCCGGG C^CCGGG XmaI 640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 168 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 576 CAGGTACTG BbsI 597 GAAGAC GAAGAC(2/6) 202 GTCTTC BcnI 640 CCCGG CC^[GC]GG 641 CCGGG BglI 496 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 913 GCCCTGCCGGC BpmI 1041 CTCCAG CTGGAG(16/14) 382 CTGGAG BpuAI 597 GAAGAC GAAGAC(2/6) 202 GTCTTC BspMI 676 ACCTGC ACCTGC(4/8) 575 GCAGGT BsrDI 516 CATTGC GCAATG(2/0) 479 GCAATG BsrFI 918 GCCGGC [GA]^CCGG[CT] 977 ACCGGC BstBI 118 TTCGAA TT^CGAA 986 TTCGAA BstYI 269 GGATCC [GA]^GATC[CT] 530 GGATCT Cfr10I 918 GCCGGC [GA]^CCGG[CT] 977 ACCGGC DraII 111 AGGCCCC [GA]G^G[ACGT]CC[CT] 843 GGGACCC EcoO109I 111 AGGCCCC [GA]G^G[ACGT]CC[CT] 843 GGGACCC FauI 258 CCCGC CCCGC(4/6) 555 CCCGC HgaI 798 GACGC GACGC(5/10) 833 GCGTC HinfI 763 GAGTC G^A[ACGT]TC 769 GAATC MflI 269 GGATCC [GA]^GATC[CT] 530 GGATCT MspA1I 259 CCGCTG C[AC]G^C[GT]G 624 CAGCGG NciI 640 CCCGG CC^[GC]GG 641 CCGGG NspV 118 TTCGAA TT^CGAA 986 TTCGAA SfuI 118 TTCGAA TT^CGAA 986 TTCGAA TaqI 119 TCGA T^CGA 987 TCGA TthHB8I 119 TCGA T^CGA 987 TCGA XhoII 269 GGATCC [GA]^GATC[CT] 530 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI BalI BbvI BsaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI