(Circular) (Six-base) MAPSORT of: pFN21AE9525.seq Check: 5893 from: 1 to: 5797 pFN21AE9525 5797 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2690 2690 Size: 5797 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 3860 5244 5512 1632 Size: 989 1239 1384 268 1917 Fragments arranged by size: 1917 1384 1239 989 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2622 4696 5673 1203 Size: 1419 2074 977 1327 Fragments arranged by size: 2074 1419 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 2178 2586 3540 5112 2178 Size: 408 954 1572 2863 Fragments arranged by size: 2863 1572 954 408 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2645 3122 1870 Size: 73 702 477 4545 Fragments arranged by size: 4545 702 477 73 BamHI G'GATC_C Cuts at: 2274 3127 3494 2274 Size: 853 367 4577 Fragments arranged by size: 4577 853 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 3116 1391 Size: 1725 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 2609 928 Size: 971 301 409 4116 Fragments arranged by size: 4116 971 409 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2507 2924 4408 Size: 107 122 71 631 605 835 417 1484 Cuts at: 4408 136 Size: 1525 Fragments arranged by size: 1525 1484 835 631 605 417 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 3858 5014 1790 Size: 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 BmgBI CAC'GTC Cuts at: 1318 2623 1318 Size: 1305 4492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3528 4486 Size: 416 53 83 399 24 651 1460 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1460 958 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 3031 4457 1239 Size: 1169 623 1426 2579 Fragments arranged by size: 2579 1426 1169 623 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 2353 2429 2436 3073 4460 882 Size: 617 804 50 76 7 637 1387 2219 Fragments arranged by size: 2219 1387 804 637 617 76 50 7 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2387 2417 1178 Size: 30 165 30 687 30 267 30 4558 Fragments arranged by size: 4558 687 267 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5797 BseYI C'CCAG_C Cuts at: 2010 2365 2844 5000 5496 2010 Size: 355 479 2156 496 2311 Fragments arranged by size: 2311 2156 496 479 355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2672 1163 Size: 573 936 4288 Fragments arranged by size: 4288 936 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2295 3247 4008 5036 1540 Size: 275 186 294 952 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 3858 3943 5014 3858 Size: 85 1071 4641 Fragments arranged by size: 4641 1071 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 BsmBI CGTCTCn'nnnn_ Cuts at: 2407 2407 Size: 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3116 3858 3943 5014 1391 Size: 399 1326 742 85 1071 2174 Fragments arranged by size: 2174 1326 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2571 2690 3152 844 Size: 1727 119 462 3489 Fragments arranged by size: 3489 1727 462 119 BsrBI CCG'CTC Cuts at: 1394 1823 2747 3684 1394 Size: 429 924 937 3507 Fragments arranged by size: 3507 937 924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 2520 4292 4466 65 Size: 1183 1243 29 1772 174 1396 Fragments arranged by size: 1772 1396 1243 1183 174 29 BsrFI r'CCGG_y Cuts at: 1700 1810 2923 2982 4441 4628 1700 Size: 110 1113 59 1459 187 2869 Fragments arranged by size: 2869 1459 1113 187 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 2873 794 Size: 2079 3718 BstBI TT'CG_AA Cuts at: 2124 2992 4601 2124 Size: 868 1609 3320 Fragments arranged by size: 3320 1609 868 BstEII G'GTnAC_C Cuts at: 1184 2677 1184 Size: 1493 4304 BstXI CCAn_nnnn'nTGG Cuts at: 2351 2351 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2274 2535 3127 3494 3879 3896 5348 5434 Size: 302 261 592 367 385 17 1452 86 Cuts at: 5434 5446 5792 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 592 385 367 346 302 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2593 513 Size: 552 939 589 3717 Fragments arranged by size: 3717 939 589 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 2302 487 Size: 683 56 902 174 3982 Fragments arranged by size: 3982 902 683 174 56 BtsI GCAGTG_nn' Cuts at: 2021 2196 3313 4107 4127 2021 Size: 175 1117 794 20 3691 Fragments arranged by size: 3691 1117 794 175 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2436 2508 Size: 54 1425 50 275 18 402 204 72 Cuts at: 2508 2736 3244 4133 5762 8 Size: 228 508 889 1629 43 Fragments arranged by size: 1629 1425 889 508 402 275 228 204 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2621 3031 3860 4457 5244 5512 Size: 393 776 213 410 829 597 787 268 Cuts at: 5512 1239 Size: 1524 Fragments arranged by size: 1524 829 787 776 597 410 393 268 213 EcoNI CCTnn'n_nnAGG Cuts at: 2452 2452 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2117 2849 2117 Size: 732 5065 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 3054 4944 1678 Size: 1376 1890 2531 Fragments arranged by size: 2531 1890 1376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 2759 2791 3020 Size: 32 135 32 835 32 320 32 229 Cuts at: 3020 3052 3056 3088 4438 4470 4512 4544 1373 Size: 32 4 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 835 320 229 135 42 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3028 3141 3387 669 Size: 988 281 1090 113 246 3079 Fragments arranged by size: 3079 1090 988 281 246 113 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2555 4911 5095 1305 Size: 1250 2356 184 2007 Fragments arranged by size: 2356 2007 1250 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2438 2510 2738 10 Size: 54 1425 343 606 72 228 3069 Fragments arranged by size: 3069 1425 606 343 228 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2813 3755 4114 4273 518 Size: 648 1647 942 359 159 2042 Fragments arranged by size: 2042 1647 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 2631 3213 3890 5038 5283 1606 Size: 660 365 582 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 582 365 245 NaeI GCC'GGC Cuts at: 2925 2925 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4306 Fragments arranged by size: 4306 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NgoMIV G'CCGG_C Cuts at: 2923 2923 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2871 3155 4700 1132 Size: 97 1642 284 1545 2229 Fragments arranged by size: 2229 1642 1545 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2178 1108 Size: 1070 4727 PfoI T'CCnGG_A Cuts at: 2385 2531 2385 Size: 146 5651 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2333 2365 3866 3898 5379 5411 2333 Size: 32 1501 32 1481 32 2719 Fragments arranged by size: 2719 1501 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2849 2849 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PstI C_TGCA'G Cuts at: 830 3097 3149 830 Size: 2267 52 3478 Fragments arranged by size: 3478 2267 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SanDI GG'GwC_CC Cuts at: 2849 2849 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SexAI A'CCwGG_T Cuts at: 2343 2343 Size: 5797 SfcI C'TryA_G Cuts at: 826 1045 3093 3145 4280 4961 5152 826 Size: 219 2048 52 1135 681 191 1471 Fragments arranged by size: 2048 1471 1135 681 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 2647 3124 1872 Size: 775 477 4545 Fragments arranged by size: 4545 775 477 SmlI C'TyrA_G Cuts at: 820 1017 1943 3901 4802 5064 820 Size: 197 926 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 2871 3155 1132 Size: 1739 284 3774 Fragments arranged by size: 3774 1739 284 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2004 3236 513 Size: 552 939 1232 3074 Fragments arranged by size: 3074 1232 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 2146 2795 3824 3841 3994 4179 Size: 1255 507 59 649 1029 17 153 185 Cuts at: 4179 4624 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1029 649 507 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2746 2746 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 2645 3122 1870 Size: 775 477 4545 Fragments arranged by size: 4545 775 477 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI