################################## # NO CUT ENZYMES ( 220 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AccI 74 GTAGAC GT^[AC][GT]AC AfaI 81 GTAC GT^AC AluI 48 AGCT AG^CT AvaI 50 CTCGGG C^[CT]CG[GA]G AvaII 196 GGACC G^G[AT]CC BsaI 56 GAGACC GGTCTC(1/5) BsaJI 39 CCTGGG C^C[ACGT][ACGT]GG BsmFI 195 GGGAC GGGAC(10/14) BsoBI 50 CTCGGG C^[CT]CG[GA]G Bsp1407I 80 TGTACA T^GTACA BsrGI 80 TGTACA T^GTACA BssSI 119 CACGAG CACGAG(-5/-1) Cfr13I 196 GGACC G^G[ACGT]CC DraII 195 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 195 GGGACCC [GA]G^G[ACGT]CC[CT] HaeIII 23 GGCC GG^CC HphI 99 GGTGA GGTGA(8/7) MboII 102 GAAGA GAAGA(8/7) MwoI 7 GCGACTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PpuMI 195 GGGACCC [GA]G^G[AT]CC[CT] PshAI 88 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 81 GTAC GT^AC Sau96I 196 GGACC G^G[ACGT]CC SexAI 59 ACCAGGT A^CC[AT]GGT SfcI 11 CTACAG C^T[GA][CT]AG SspBI 80 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BpmI 126 CTCCAG CTGGAG(16/14) 69 CTGGAG BsmAI 56 GAGAC GTCTC(1/5) 86 GAGAC BsrI 68 ACTGG ACTGG(1/-1) 91 ACTGG MnlI 125 CCTC CCTC(7/6) 165 GAGG TspRI 33 CACTG CA[GC]TG[ACGT][ACGT]^ 90 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BssKI BstNI Cac8I EcoRII MvaI NlaIV ScrFI