(Circular) (Six-base) MAPSORT of: pFN21AE6510.seq Check: 749 from: 1 to: 4939 pFN21AE6510 4939 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2749 278 Size: 53 83 186 936 1213 2468 Fragments arranged by size: 2468 1213 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2080 2282 1937 Size: 143 202 4594 Fragments arranged by size: 4594 202 143 Acc65I G'GTAC_C Cuts at: 2260 2260 Size: 4939 AclI AA'CG_TT Cuts at: 1563 3066 3439 1563 Size: 1503 373 3063 Fragments arranged by size: 3063 1503 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3002 4386 4654 1632 Size: 1370 1384 268 1917 Fragments arranged by size: 1917 1384 1370 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4742 AflIII A'CryG_T Cuts at: 1203 3838 4815 1203 Size: 2635 977 1327 Fragments arranged by size: 2635 1327 977 AgeI A'CCGG_T Cuts at: 3770 3770 Size: 4939 AhdI GACnn_n'nnGTC Cuts at: 3668 3668 Size: 4939 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4939 AlwNI CAG_nnn'CTG Cuts at: 2682 4254 2682 Size: 1572 3367 ApaLI G'TGCA_C Cuts at: 2996 4152 2996 Size: 1156 3783 ApoI r'AATT_y Cuts at: 1493 2248 2474 2626 4735 1493 Size: 755 226 152 2109 1697 Fragments arranged by size: 2109 1697 755 226 152 AseI AT'TA_AT Cuts at: 160 1032 3493 160 Size: 872 2461 1606 Fragments arranged by size: 2461 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4939 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2264 1870 Size: 73 112 209 4545 Fragments arranged by size: 4545 209 112 73 BamHI G'GATC_C Cuts at: 2269 2636 2269 Size: 367 4572 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 3715 618 Size: 325 217 627 473 1455 1842 Fragments arranged by size: 1842 1455 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2258 1391 Size: 867 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3968 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3130 3164 1903 Size: 34 1193 34 3678 Fragments arranged by size: 3678 1193 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2831 4041 2831 Size: 1210 3729 BclI T'GATC_A Cuts at: 1548 1548 Size: 4939 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3550 136 Size: 107 122 71 631 605 337 1541 1525 Fragments arranged by size: 1541 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4934 4934 Size: 4939 BlpI GC'TnA_GC Cuts at: 2356 2356 Size: 4939 Bme1580I G_kGCm'C Cuts at: 1790 3000 4156 1790 Size: 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4939 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2670 3628 442 Size: 416 53 83 399 24 1253 958 1753 Fragments arranged by size: 1753 1253 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4939 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2116 3599 1239 Size: 856 21 1483 2579 Fragments arranged by size: 2579 1483 856 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3064 3929 4227 3064 Size: 865 298 3776 Fragments arranged by size: 3776 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2055 3602 882 Size: 617 556 1547 2219 Fragments arranged by size: 2219 1547 617 556 BsaAI yAC'GTr Cuts at: 493 1204 2730 493 Size: 711 1526 2702 Fragments arranged by size: 2702 1526 711 BsaBI GATnn'nnATC Cuts at: 4933 4933 Size: 4939 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2746 3128 3763 275 Size: 53 83 186 936 1213 382 635 1451 Fragments arranged by size: 1451 1213 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3372 3770 4044 4191 4602 3372 Size: 398 274 147 411 3709 Fragments arranged by size: 3709 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2052 2082 1178 Size: 30 165 30 649 30 4035 Fragments arranged by size: 4035 649 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4939 BseYI C'CCAG_C Cuts at: 4142 4638 4142 Size: 496 4443 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2389 3150 4178 1540 Size: 275 186 388 761 1028 2301 Fragments arranged by size: 2301 1028 761 388 275 186 BsiHKAI G_wGCw'C Cuts at: 3000 3085 4156 3000 Size: 85 1071 3783 Fragments arranged by size: 3783 1071 85 BsmI GAATG_Cn' Cuts at: 2450 2543 2450 Size: 93 4846 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2258 3000 3085 4156 1391 Size: 399 468 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 468 399 85 BspEI T'CCGG_A Cuts at: 4602 4602 Size: 4939 BspHI T'CATG_A Cuts at: 2828 4558 2828 Size: 1730 3209 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 844 Size: 1450 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2826 1394 Size: 429 1003 3507 Fragments arranged by size: 3507 1003 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3434 3608 65 Size: 1183 2186 174 1396 Fragments arranged by size: 2186 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3583 3770 1700 Size: 110 1773 187 2869 Fragments arranged by size: 2869 1773 187 110 BsrGI T'GTAC_A Cuts at: 96 2085 96 Size: 1989 2950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4939 BssSI C'ACGA_G Cuts at: 2124 2999 4011 2124 Size: 875 1012 3052 Fragments arranged by size: 3052 1012 875 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4939 BstBI TT'CG_AA Cuts at: 3743 3743 Size: 4939 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4939 BstYI r'GATC_y Cuts at: 1972 2269 2636 3021 3038 4490 4576 4588 4934 Size: 297 367 385 17 1452 86 12 346 Cuts at: 4934 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 297 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4387 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4200 Fragments arranged by size: 4200 683 56 BtsI GCAGTG_nn' Cuts at: 2455 3249 3269 2455 Size: 794 20 4125 Fragments arranged by size: 4125 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4939 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4904 DraI TTT'AAA Cuts at: 2243 2243 Size: 4939 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3946 809 Size: 507 2630 1802 Fragments arranged by size: 2630 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2386 3275 4904 Size: 54 1425 50 275 18 556 889 1629 Cuts at: 4904 8 Size: 43 Fragments arranged by size: 1629 1425 889 556 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2386 1537 Size: 275 574 4090 Fragments arranged by size: 4090 574 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2869 1353 Size: 1516 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3526 3900 4046 387 Size: 1064 138 1937 374 146 1280 Fragments arranged by size: 1937 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2116 3002 3599 4386 4654 1239 Size: 393 463 21 886 597 787 268 1524 Fragments arranged by size: 1524 886 787 597 463 393 268 21 EcoO109I rG'GnC_Cy Cuts at: 2201 2201 Size: 4939 EcoRI G'AATT_C Cuts at: 2248 2626 2248 Size: 378 4561 FspI TGC'GCA Cuts at: 3445 3445 Size: 4939 HaeII r_GCGC'y Cuts at: 1678 4086 1678 Size: 2408 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3580 3612 3654 3686 1373 Size: 32 135 32 2008 32 42 32 2626 Fragments arranged by size: 2626 2008 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2283 2529 669 Size: 988 281 345 246 3079 Fragments arranged by size: 3079 988 345 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4939 HpaI GTT'AAC Cuts at: 2529 2529 Size: 4939 KpnI G_GTAC'C Cuts at: 2264 2264 Size: 4939 MfeI C'AATT_G Cuts at: 2538 2538 Size: 4939 MluI A'CGCG_T Cuts at: 4815 4815 Size: 4939 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 4053 4237 1305 Size: 864 1884 184 2007 Fragments arranged by size: 2007 1884 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3117 Fragments arranged by size: 3117 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 3256 3415 518 Size: 648 1731 359 159 2042 Fragments arranged by size: 2042 1731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2355 3032 4180 4425 1606 Size: 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4387 NdeI CA'TA_TG Cuts at: 387 387 Size: 4939 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4939 NotI GC'GGCC_GC Cuts at: 2386 2386 Size: 4939 NspI r_CATG'y Cuts at: 1132 1229 2297 3842 1132 Size: 97 1068 1545 2229 Fragments arranged by size: 2229 1545 1068 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4939 PciI A'CATG_T Cuts at: 3838 3838 Size: 4939 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4939 PfoI T'CCnGG_A Cuts at: 2114 2218 2114 Size: 104 4835 PmeI GTTT'AAAC Cuts at: 2243 2243 Size: 4939 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3008 3040 4521 4553 3008 Size: 32 1481 32 3394 Fragments arranged by size: 3394 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2201 Size: 4939 PshAI GACnn'nnGTC Cuts at: 1613 2097 1613 Size: 484 4455 PsiI TTA'TAA Cuts at: 2509 2509 Size: 4939 PstI C_TGCA'G Cuts at: 830 2291 830 Size: 1461 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4939 SalI G'TCGA_C Cuts at: 1936 2281 1936 Size: 345 4594 SanDI GG'GwC_CC Cuts at: 2201 2201 Size: 4939 SbfI CC_TGCA'GG Cuts at: 2291 2291 Size: 4939 ScaI AGT'ACT Cuts at: 3187 4899 3187 Size: 1712 3227 SexAI A'CCwGG_T Cuts at: 2064 2064 Size: 4939 SfcI C'TryA_G Cuts at: 826 1045 2016 2287 3422 4103 4294 826 Size: 219 971 271 1135 681 191 1471 Fragments arranged by size: 1471 1135 971 681 271 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4939 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4939 SmaI CCC'GGG Cuts at: 1872 2266 1872 Size: 394 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3043 3944 4206 820 Size: 197 926 1100 901 262 1553 Fragments arranged by size: 1553 1100 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2730 493 Size: 2237 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4939 SphI G_CATG'C Cuts at: 1132 2297 1132 Size: 1165 3774 SspI AAT'ATT Cuts at: 5 52 2863 5 Size: 47 2811 2081 Fragments arranged by size: 2811 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4939 StyI C'CwwG_G Cuts at: 513 1065 2378 513 Size: 552 1313 3074 Fragments arranged by size: 3074 1313 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2169 2966 2983 3136 3321 3766 325 Size: 1255 589 797 17 153 185 445 1498 Fragments arranged by size: 1498 1255 797 589 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 3185 4897 Size: 274 80 33 51 1443 108 1100 1712 Cuts at: 4897 96 Size: 138 Fragments arranged by size: 1712 1443 1100 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2052 2084 4899 4931 2052 Size: 32 2815 32 2060 Fragments arranged by size: 2815 2060 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4939 XbaI T'CTAG_A Cuts at: 2275 2275 Size: 4939 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4939 XmaI C'CCGG_G Cuts at: 1870 2264 1870 Size: 394 4545 XmnI GAAnn'nnTTC Cuts at: 3068 3068 Size: 4939 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2747 276 Size: 53 83 186 936 1213 2468 Fragments arranged by size: 2468 1213 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI